Biosynthesis of silver nanoparticles using nitrate reductase produced by Lactobacillus plantarum CAM 4: characterization and in vitro evaluation of its antimicrobial efficiency
Among emerging nanotechnology, silver nanoparticles (AgNPs) get much attention due to their unique physicochemical properties. The present study aimed to optimize the nitrate reductase production in lactic acid bacteria (LAB) for enhanced production of AgNPs and characterize the biosynthesized AgNPs...
| Main Authors: | , , , , , , , |
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| Format: | Article |
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Elsevier
2023
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| Online Access: | http://psasir.upm.edu.my/id/eprint/106901/ |
| _version_ | 1848864848074506240 |
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| author | Ren Loi, Hsean Abbasiliasi, Sahar Bothi Raja, Pandian Shamzi Mohamed, Mohd Tan, Wen-Nee Suan Ng, Hui Chi-Wei Lan, John Shun Tan, Joo |
| author_facet | Ren Loi, Hsean Abbasiliasi, Sahar Bothi Raja, Pandian Shamzi Mohamed, Mohd Tan, Wen-Nee Suan Ng, Hui Chi-Wei Lan, John Shun Tan, Joo |
| author_sort | Ren Loi, Hsean |
| building | UPM Institutional Repository |
| collection | Online Access |
| description | Among emerging nanotechnology, silver nanoparticles (AgNPs) get much attention due to their unique physicochemical properties. The present study aimed to optimize the nitrate reductase production in lactic acid bacteria (LAB) for enhanced production of AgNPs and characterize the biosynthesized AgNPs. Lactobacillus plantarum CAM 4 produced higher nitrate reductase activity (121.77 U/mL) under an optimized medium containing 0.75 of glucose, 2 of KNO3, and pH 5.0 and successfully enhanced the AgNPs biosynthesis. Spectrophotometric analysis revealed that the biosynthesized AgNPs exhibited a peak at 430 nm. DLS analysis indicated the monodispersed characteristic of AgNPs. TEM and FE-SEM analysis shows that the AgNPs were spherical-like shape, while the EDX analysis spectrum revealed the existence of an Ag element in AgNPs. Moreover, biosynthesized AgNPs exhibited antibacterial activity against Gram-positive and Gram-negative pathogens. Results from this study provide notable insights into sustainable and inexpensive methods for the enhanced production of AgNPs using LAB. |
| first_indexed | 2025-11-15T13:55:20Z |
| format | Article |
| id | upm-106901 |
| institution | Universiti Putra Malaysia |
| institution_category | Local University |
| last_indexed | 2025-11-15T13:55:20Z |
| publishDate | 2023 |
| publisher | Elsevier |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | upm-1069012024-08-05T08:42:56Z http://psasir.upm.edu.my/id/eprint/106901/ Biosynthesis of silver nanoparticles using nitrate reductase produced by Lactobacillus plantarum CAM 4: characterization and in vitro evaluation of its antimicrobial efficiency Ren Loi, Hsean Abbasiliasi, Sahar Bothi Raja, Pandian Shamzi Mohamed, Mohd Tan, Wen-Nee Suan Ng, Hui Chi-Wei Lan, John Shun Tan, Joo Among emerging nanotechnology, silver nanoparticles (AgNPs) get much attention due to their unique physicochemical properties. The present study aimed to optimize the nitrate reductase production in lactic acid bacteria (LAB) for enhanced production of AgNPs and characterize the biosynthesized AgNPs. Lactobacillus plantarum CAM 4 produced higher nitrate reductase activity (121.77 U/mL) under an optimized medium containing 0.75 of glucose, 2 of KNO3, and pH 5.0 and successfully enhanced the AgNPs biosynthesis. Spectrophotometric analysis revealed that the biosynthesized AgNPs exhibited a peak at 430 nm. DLS analysis indicated the monodispersed characteristic of AgNPs. TEM and FE-SEM analysis shows that the AgNPs were spherical-like shape, while the EDX analysis spectrum revealed the existence of an Ag element in AgNPs. Moreover, biosynthesized AgNPs exhibited antibacterial activity against Gram-positive and Gram-negative pathogens. Results from this study provide notable insights into sustainable and inexpensive methods for the enhanced production of AgNPs using LAB. Elsevier 2023 Article PeerReviewed Ren Loi, Hsean and Abbasiliasi, Sahar and Bothi Raja, Pandian and Shamzi Mohamed, Mohd and Tan, Wen-Nee and Suan Ng, Hui and Chi-Wei Lan, John and Shun Tan, Joo (2023) Biosynthesis of silver nanoparticles using nitrate reductase produced by Lactobacillus plantarum CAM 4: characterization and in vitro evaluation of its antimicrobial efficiency. Journal of Molecular Liquids, 376. pp. 1-14. ISSN 0167-7322; ESSN: 1873-3166 https://linkinghub.elsevier.com/retrieve/pii/S0167732223002799 10.1016/j.molliq.2023.121476 |
| spellingShingle | Ren Loi, Hsean Abbasiliasi, Sahar Bothi Raja, Pandian Shamzi Mohamed, Mohd Tan, Wen-Nee Suan Ng, Hui Chi-Wei Lan, John Shun Tan, Joo Biosynthesis of silver nanoparticles using nitrate reductase produced by Lactobacillus plantarum CAM 4: characterization and in vitro evaluation of its antimicrobial efficiency |
| title | Biosynthesis of silver nanoparticles using nitrate reductase produced by Lactobacillus plantarum CAM 4: characterization and in vitro evaluation of its antimicrobial efficiency |
| title_full | Biosynthesis of silver nanoparticles using nitrate reductase produced by Lactobacillus plantarum CAM 4: characterization and in vitro evaluation of its antimicrobial efficiency |
| title_fullStr | Biosynthesis of silver nanoparticles using nitrate reductase produced by Lactobacillus plantarum CAM 4: characterization and in vitro evaluation of its antimicrobial efficiency |
| title_full_unstemmed | Biosynthesis of silver nanoparticles using nitrate reductase produced by Lactobacillus plantarum CAM 4: characterization and in vitro evaluation of its antimicrobial efficiency |
| title_short | Biosynthesis of silver nanoparticles using nitrate reductase produced by Lactobacillus plantarum CAM 4: characterization and in vitro evaluation of its antimicrobial efficiency |
| title_sort | biosynthesis of silver nanoparticles using nitrate reductase produced by lactobacillus plantarum cam 4: characterization and in vitro evaluation of its antimicrobial efficiency |
| url | http://psasir.upm.edu.my/id/eprint/106901/ http://psasir.upm.edu.my/id/eprint/106901/ http://psasir.upm.edu.my/id/eprint/106901/ |