Biodegradation of expanded and extruded polystyrene with different diets by using Zophobas atratus larvae (Coleoptera: tenebrionidae)
Polystyrene waste polluted the environment and poses a significant health risk to humans, animals and the marine ecology. This study aims to evaluate the effectiveness of degradation on expanded and extruded polystyrene with different diets using superworms (Zophobas atratus larvae) obtained in Mala...
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| Format: | Article |
| Language: | English |
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Universiti Putra Malaysia
2023
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| Online Access: | http://psasir.upm.edu.my/id/eprint/106881/ http://psasir.upm.edu.my/id/eprint/106881/1/Biodegradation%20of%20Expanded%20and%20Extruded%20Polystyrene.pdf.crdownload |
| _version_ | 1848864842819043328 |
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| author | Hoe Tay, Jun Asib, Norhayu Abd Aziz, Nor Azwady Hun Tan, Geok |
| author_facet | Hoe Tay, Jun Asib, Norhayu Abd Aziz, Nor Azwady Hun Tan, Geok |
| author_sort | Hoe Tay, Jun |
| building | UPM Institutional Repository |
| collection | Online Access |
| description | Polystyrene waste polluted the environment and poses a significant health risk to humans, animals and the marine ecology. This study aims to evaluate the effectiveness of degradation on expanded and extruded polystyrene with different diets using superworms (Zophobas atratus larvae) obtained in Malaysia. The growth and development of the larvae after consumption of expanded (EPS) and extruded (XPS) polystyrene, and the gut's microbial community changes in response to high polystyrene consumption diets were also identified. The oatmeal, wheat bran, and cornmeal were used as supplements diets showed significantly enhanced EPS and XPS consumption and degradation compared to sole diet treatment. Gel permeation chromatography (GPC) was carried out using egested frass of Z. atratus larvae to characterize depolymerization of EPS and XPS which indicated significant reduction in both Mn and Mw of EPS and XPS. The highest reduction occurred in the presence of co-diet oatmeal. 1H NMR and FTIR analyses results indicated functional group changes and chemical modification occurred with depolymerization and partial oxidation of EPS and XPS. The larvae length increased, while the number of instars and duration of larvae became shorter with the addition of supplements diets. Oatmeal is predominantly effective among other supplements in assisting Z. atratus larvae on EPS and XPS degradation. The results of this study support ubiquity of PS biodegradation in Z. atratus, and the next generation sequencing studies. Kluyvera sp., Klebsiella sp. and Enterobacter sp. were found strongly associated in degrading EPS and XPS polystyrene with oatmeal as a supplemental diet. |
| first_indexed | 2025-11-15T13:55:15Z |
| format | Article |
| id | upm-106881 |
| institution | Universiti Putra Malaysia |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-15T13:55:15Z |
| publishDate | 2023 |
| publisher | Universiti Putra Malaysia |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | upm-1068812024-08-06T02:23:53Z http://psasir.upm.edu.my/id/eprint/106881/ Biodegradation of expanded and extruded polystyrene with different diets by using Zophobas atratus larvae (Coleoptera: tenebrionidae) Hoe Tay, Jun Asib, Norhayu Abd Aziz, Nor Azwady Hun Tan, Geok Polystyrene waste polluted the environment and poses a significant health risk to humans, animals and the marine ecology. This study aims to evaluate the effectiveness of degradation on expanded and extruded polystyrene with different diets using superworms (Zophobas atratus larvae) obtained in Malaysia. The growth and development of the larvae after consumption of expanded (EPS) and extruded (XPS) polystyrene, and the gut's microbial community changes in response to high polystyrene consumption diets were also identified. The oatmeal, wheat bran, and cornmeal were used as supplements diets showed significantly enhanced EPS and XPS consumption and degradation compared to sole diet treatment. Gel permeation chromatography (GPC) was carried out using egested frass of Z. atratus larvae to characterize depolymerization of EPS and XPS which indicated significant reduction in both Mn and Mw of EPS and XPS. The highest reduction occurred in the presence of co-diet oatmeal. 1H NMR and FTIR analyses results indicated functional group changes and chemical modification occurred with depolymerization and partial oxidation of EPS and XPS. The larvae length increased, while the number of instars and duration of larvae became shorter with the addition of supplements diets. Oatmeal is predominantly effective among other supplements in assisting Z. atratus larvae on EPS and XPS degradation. The results of this study support ubiquity of PS biodegradation in Z. atratus, and the next generation sequencing studies. Kluyvera sp., Klebsiella sp. and Enterobacter sp. were found strongly associated in degrading EPS and XPS polystyrene with oatmeal as a supplemental diet. Universiti Putra Malaysia 2023 Article PeerReviewed text en http://psasir.upm.edu.my/id/eprint/106881/1/Biodegradation%20of%20Expanded%20and%20Extruded%20Polystyrene.pdf.crdownload Hoe Tay, Jun and Asib, Norhayu and Abd Aziz, Nor Azwady and Hun Tan, Geok (2023) Biodegradation of expanded and extruded polystyrene with different diets by using Zophobas atratus larvae (Coleoptera: tenebrionidae). Pertanika Journal of Tropical Agricultural Science, 46 (2). pp. 459-483. ISSN 1511-3701; ESSN: 2231-8542 http://www.pertanika.upm.edu.my/pjtas/browse/regular-issue?article=JTAS-2578-2022 10.47836/pjtas.46.2.06 |
| spellingShingle | Hoe Tay, Jun Asib, Norhayu Abd Aziz, Nor Azwady Hun Tan, Geok Biodegradation of expanded and extruded polystyrene with different diets by using Zophobas atratus larvae (Coleoptera: tenebrionidae) |
| title | Biodegradation of expanded and extruded polystyrene with different diets by using Zophobas atratus larvae (Coleoptera: tenebrionidae) |
| title_full | Biodegradation of expanded and extruded polystyrene with different diets by using Zophobas atratus larvae (Coleoptera: tenebrionidae) |
| title_fullStr | Biodegradation of expanded and extruded polystyrene with different diets by using Zophobas atratus larvae (Coleoptera: tenebrionidae) |
| title_full_unstemmed | Biodegradation of expanded and extruded polystyrene with different diets by using Zophobas atratus larvae (Coleoptera: tenebrionidae) |
| title_short | Biodegradation of expanded and extruded polystyrene with different diets by using Zophobas atratus larvae (Coleoptera: tenebrionidae) |
| title_sort | biodegradation of expanded and extruded polystyrene with different diets by using zophobas atratus larvae (coleoptera: tenebrionidae) |
| url | http://psasir.upm.edu.my/id/eprint/106881/ http://psasir.upm.edu.my/id/eprint/106881/ http://psasir.upm.edu.my/id/eprint/106881/ http://psasir.upm.edu.my/id/eprint/106881/1/Biodegradation%20of%20Expanded%20and%20Extruded%20Polystyrene.pdf.crdownload |