Association of LDLR, TP53 and MMP9 gene polymorphisms with atherosclerosis in a Malaysian study population

Preliminary research has shown that low density lipoprotein receptor (LDLR), tumor protein (TP53) and matrix metalloproteinase 9 (MMP9) genes expression levels were significantly increased in atherosclerosis coronary artery tissue (ACAT) compared to non-atherosclerotic coronary artery tissue (NCAT)...

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Main Authors: Ahmad, Zalinah, Blin, Joan, Rampal, Lekhraj, Adnan, Rohayu Shahar, Mohtarrudin, Norhafizah, Abang Yusuf, Dayang Sarah Yasmine
Format: Article
Published: Elsevier 2023
Online Access:http://psasir.upm.edu.my/id/eprint/106763/
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author Ahmad, Zalinah
Blin, Joan
Rampal, Lekhraj
Adnan, Rohayu Shahar
Mohtarrudin, Norhafizah
Abang Yusuf, Dayang Sarah Yasmine
author_facet Ahmad, Zalinah
Blin, Joan
Rampal, Lekhraj
Adnan, Rohayu Shahar
Mohtarrudin, Norhafizah
Abang Yusuf, Dayang Sarah Yasmine
author_sort Ahmad, Zalinah
building UPM Institutional Repository
collection Online Access
description Preliminary research has shown that low density lipoprotein receptor (LDLR), tumor protein (TP53) and matrix metalloproteinase 9 (MMP9) genes expression levels were significantly increased in atherosclerosis coronary artery tissue (ACAT) compared to non-atherosclerotic coronary artery tissue (NCAT) samples. Thus, further investigation was carried out to study the association of LDLR, TP53 and MMP9 gene polymorphisms and the risk of developing atherosclerosis (ATH) in a Malaysian population. Single nucleotide polymorphisms of C88S, TP53 codon 72 and MMP9C>T were analyzed in 76 ACAT samples and 149 NCAT samples, representing cases and controls, respectively. In results, heterozygous CT genotype of MMP9C>T polymorphism was significantly higher in ACAT compared to NCAT samples (57.9 vs 27.5, ‡2 = 19.758, df= 1, P < 0.05). The CT genotype was found to be significantly associated with the risk of developing ATH (OR = 3.622, 95 CI = 2.028-6.470). However, the distribution of the CT genotype in a healthy Malaysian study population was incomparable regardless of gender and ethnicity. The DNA sequencing results validated the C88S, TP53 codon 72, and MMP9C>T polymorphisms. In conclusion, the CT genotype of the MMP9-1562C>T polymorphism was found to have a strong association with the risk of developing ATH.
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spelling upm-1067632024-09-26T07:47:13Z http://psasir.upm.edu.my/id/eprint/106763/ Association of LDLR, TP53 and MMP9 gene polymorphisms with atherosclerosis in a Malaysian study population Ahmad, Zalinah Blin, Joan Rampal, Lekhraj Adnan, Rohayu Shahar Mohtarrudin, Norhafizah Abang Yusuf, Dayang Sarah Yasmine Preliminary research has shown that low density lipoprotein receptor (LDLR), tumor protein (TP53) and matrix metalloproteinase 9 (MMP9) genes expression levels were significantly increased in atherosclerosis coronary artery tissue (ACAT) compared to non-atherosclerotic coronary artery tissue (NCAT) samples. Thus, further investigation was carried out to study the association of LDLR, TP53 and MMP9 gene polymorphisms and the risk of developing atherosclerosis (ATH) in a Malaysian population. Single nucleotide polymorphisms of C88S, TP53 codon 72 and MMP9C>T were analyzed in 76 ACAT samples and 149 NCAT samples, representing cases and controls, respectively. In results, heterozygous CT genotype of MMP9C>T polymorphism was significantly higher in ACAT compared to NCAT samples (57.9 vs 27.5, ‡2 = 19.758, df= 1, P < 0.05). The CT genotype was found to be significantly associated with the risk of developing ATH (OR = 3.622, 95 CI = 2.028-6.470). However, the distribution of the CT genotype in a healthy Malaysian study population was incomparable regardless of gender and ethnicity. The DNA sequencing results validated the C88S, TP53 codon 72, and MMP9C>T polymorphisms. In conclusion, the CT genotype of the MMP9-1562C>T polymorphism was found to have a strong association with the risk of developing ATH. Elsevier 2023 Article PeerReviewed Ahmad, Zalinah and Blin, Joan and Rampal, Lekhraj and Adnan, Rohayu Shahar and Mohtarrudin, Norhafizah and Abang Yusuf, Dayang Sarah Yasmine (2023) Association of LDLR, TP53 and MMP9 gene polymorphisms with atherosclerosis in a Malaysian study population. Current Problems in Cardiology, 48 (6). art. no. 101659. pp. 1-21. ISSN 0146-2806; ESSN: 1535-6280 https://www.sciencedirect.com/science/article/pii/S0146280623000762 10.1016/j.cpcardiol.2023.101659
spellingShingle Ahmad, Zalinah
Blin, Joan
Rampal, Lekhraj
Adnan, Rohayu Shahar
Mohtarrudin, Norhafizah
Abang Yusuf, Dayang Sarah Yasmine
Association of LDLR, TP53 and MMP9 gene polymorphisms with atherosclerosis in a Malaysian study population
title Association of LDLR, TP53 and MMP9 gene polymorphisms with atherosclerosis in a Malaysian study population
title_full Association of LDLR, TP53 and MMP9 gene polymorphisms with atherosclerosis in a Malaysian study population
title_fullStr Association of LDLR, TP53 and MMP9 gene polymorphisms with atherosclerosis in a Malaysian study population
title_full_unstemmed Association of LDLR, TP53 and MMP9 gene polymorphisms with atherosclerosis in a Malaysian study population
title_short Association of LDLR, TP53 and MMP9 gene polymorphisms with atherosclerosis in a Malaysian study population
title_sort association of ldlr, tp53 and mmp9 gene polymorphisms with atherosclerosis in a malaysian study population
url http://psasir.upm.edu.my/id/eprint/106763/
http://psasir.upm.edu.my/id/eprint/106763/
http://psasir.upm.edu.my/id/eprint/106763/