Assessing indigenous soil ureolytic bacteria as potential agents for soil stabilization
Microbially induced carbonate precipitation by ureolysis is a biomineralization process that has been adapted by various microorganisms in different natural environments. This widespread natural phenomenon can be employed in numerous civil engineering and soil stabilization applications. In the pres...
| Main Authors: | , , , , |
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| Format: | Article |
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Universitas Gadjah Mada
2023
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| Online Access: | http://psasir.upm.edu.my/id/eprint/106723/ |
| _version_ | 1848864811836768256 |
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| author | Aliyu, Abdulaziz Dardau Mustafa, Muskhazli Aziz, Nor Azwady Abd Kong, Yap Chee Hadi, Najaatu Shehu |
| author_facet | Aliyu, Abdulaziz Dardau Mustafa, Muskhazli Aziz, Nor Azwady Abd Kong, Yap Chee Hadi, Najaatu Shehu |
| author_sort | Aliyu, Abdulaziz Dardau |
| building | UPM Institutional Repository |
| collection | Online Access |
| description | Microbially induced carbonate precipitation by ureolysis is a biomineralization process that has been adapted by various microorganisms in different natural environments. This widespread natural phenomenon can be employed in numerous civil engineering and soil stabilization applications. In the present study, the potential of indigenous soil urease-producing bacteria as potential agents for soil stabilization method was investigated. Assessment of the eight active urease-producing bacterial species isolated from the farm soil samples has demonstrated that all the isolates were Gram-positive rod-shaped bacteria with promising characteristics such as the formation of endospore which is essential for bacterial survival in harsh conditions within the soil environment. The pH profile and growth profile of the isolates were studied and urease activity was measured by phenol hypochlorite assay method. Two isolates designated isolate O6w and isolate O3a were selected based on the highest urease activity recorded at 665 U/mL and 620 U/mL, respectively, and they were able to increase and sustain alkaline culture condition (pH 8.71 ± 0.01 and 8.55 ± 0.01) which was suitable for CaCO3 precipitation. The isolates were identified based on 16S ribosomal RNA sequencing to be Bacillus cereus (O6w) and Bacillus paramycoides (O3a). This current study suggested that indigenous soil ureolytic bacteria are potential raw material for the biotreatment of soils stability. |
| first_indexed | 2025-11-15T13:54:45Z |
| format | Article |
| id | upm-106723 |
| institution | Universiti Putra Malaysia |
| institution_category | Local University |
| last_indexed | 2025-11-15T13:54:45Z |
| publishDate | 2023 |
| publisher | Universitas Gadjah Mada |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | upm-1067232024-10-03T04:40:08Z http://psasir.upm.edu.my/id/eprint/106723/ Assessing indigenous soil ureolytic bacteria as potential agents for soil stabilization Aliyu, Abdulaziz Dardau Mustafa, Muskhazli Aziz, Nor Azwady Abd Kong, Yap Chee Hadi, Najaatu Shehu Microbially induced carbonate precipitation by ureolysis is a biomineralization process that has been adapted by various microorganisms in different natural environments. This widespread natural phenomenon can be employed in numerous civil engineering and soil stabilization applications. In the present study, the potential of indigenous soil urease-producing bacteria as potential agents for soil stabilization method was investigated. Assessment of the eight active urease-producing bacterial species isolated from the farm soil samples has demonstrated that all the isolates were Gram-positive rod-shaped bacteria with promising characteristics such as the formation of endospore which is essential for bacterial survival in harsh conditions within the soil environment. The pH profile and growth profile of the isolates were studied and urease activity was measured by phenol hypochlorite assay method. Two isolates designated isolate O6w and isolate O3a were selected based on the highest urease activity recorded at 665 U/mL and 620 U/mL, respectively, and they were able to increase and sustain alkaline culture condition (pH 8.71 ± 0.01 and 8.55 ± 0.01) which was suitable for CaCO3 precipitation. The isolates were identified based on 16S ribosomal RNA sequencing to be Bacillus cereus (O6w) and Bacillus paramycoides (O3a). This current study suggested that indigenous soil ureolytic bacteria are potential raw material for the biotreatment of soils stability. Universitas Gadjah Mada 2023 Article PeerReviewed Aliyu, Abdulaziz Dardau and Mustafa, Muskhazli and Aziz, Nor Azwady Abd and Kong, Yap Chee and Hadi, Najaatu Shehu (2023) Assessing indigenous soil ureolytic bacteria as potential agents for soil stabilization. Journal of Tropical Biodiversity and Biotechnology, 8 (1). pp. 1-20. ISSN 2540-9573; eISSN: 2540-9581 https://jurnal.ugm.ac.id/jtbb/article/view/75128 10.22146/jtbb.75128 |
| spellingShingle | Aliyu, Abdulaziz Dardau Mustafa, Muskhazli Aziz, Nor Azwady Abd Kong, Yap Chee Hadi, Najaatu Shehu Assessing indigenous soil ureolytic bacteria as potential agents for soil stabilization |
| title | Assessing indigenous soil ureolytic bacteria as potential agents for soil stabilization |
| title_full | Assessing indigenous soil ureolytic bacteria as potential agents for soil stabilization |
| title_fullStr | Assessing indigenous soil ureolytic bacteria as potential agents for soil stabilization |
| title_full_unstemmed | Assessing indigenous soil ureolytic bacteria as potential agents for soil stabilization |
| title_short | Assessing indigenous soil ureolytic bacteria as potential agents for soil stabilization |
| title_sort | assessing indigenous soil ureolytic bacteria as potential agents for soil stabilization |
| url | http://psasir.upm.edu.my/id/eprint/106723/ http://psasir.upm.edu.my/id/eprint/106723/ http://psasir.upm.edu.my/id/eprint/106723/ |