Apoptotic potential of glucomoringin isothiocyanate (GMG-ITC) isolated from Moringa oleifera Lam seeds on human prostate cancer cells (PC-3)
Inhibition of several protein pathways involved in cancer cell regulation is a necessary key in the discovery of cancer chemotherapy. Moringa oleifera Lam is often used in traditional medicine for the treatment of various illnesses. The plant contains glucomoringin isothiocyanate (GMG-ITC) with ther...
| Main Authors: | , , , , |
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| Format: | Article |
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Multidisciplinary Digital Publishing Institute
2023
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| Online Access: | http://psasir.upm.edu.my/id/eprint/106635/ |
| _version_ | 1848864795002929152 |
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| author | Abd Karim, Nurul Ashikin Adam, Aziza Hussein Bakheit Jaafaru, Mohammed Sani Rukayadi, Yaya Abdull Razis, Ahmad Faizal |
| author_facet | Abd Karim, Nurul Ashikin Adam, Aziza Hussein Bakheit Jaafaru, Mohammed Sani Rukayadi, Yaya Abdull Razis, Ahmad Faizal |
| author_sort | Abd Karim, Nurul Ashikin |
| building | UPM Institutional Repository |
| collection | Online Access |
| description | Inhibition of several protein pathways involved in cancer cell regulation is a necessary key in the discovery of cancer chemotherapy. Moringa oleifera Lam is often used in traditional medicine for the treatment of various illnesses. The plant contains glucomoringin isothiocyanate (GMG-ITC) with therapeutic potential against various cancer cells. Therefore, GMG-ITC was evaluated for its cytotoxicity against the PC-3 prostate cancer cell line and its potential to induce apoptosis. GMG-ITC inhibited cell proliferation in the PC-3 cell line with IC50 value 3.5 µg/mL. Morphological changes as a result of GMG-ITC-induced apoptosis showed chromatin condensation, nuclear fragmentation, and membrane blebbing. Additionally, Annexin V assay showed proportion of cells in early and late apoptosis upon exposure to GMG-ITC in a time-dependent manner. Moreover, GMG-ITC induced a time-dependent G2/M phase arrest, with reduction of 39.1 in the PC-3 cell line. GMG-ITC also activates apoptotic genes including caspase, tumor suppressor gene (p53), Akt/MAPK, and Bax of the proapoptotic Bcl family. Early apoptosis proteins (JNK, Bad, Bcl2, and p53) were significantly upregulated upon GMG-ITC treatment. It is concluded that apoptosis induction was observed in PC-3 cells treated with GMG-ITC. These phenomena suggest that GMG-ITC from M. oleifera seeds could be useful as a future cytotoxic agent against prostate cancer. |
| first_indexed | 2025-11-15T13:54:29Z |
| format | Article |
| id | upm-106635 |
| institution | Universiti Putra Malaysia |
| institution_category | Local University |
| last_indexed | 2025-11-15T13:54:29Z |
| publishDate | 2023 |
| publisher | Multidisciplinary Digital Publishing Institute |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | upm-1066352024-09-26T08:32:52Z http://psasir.upm.edu.my/id/eprint/106635/ Apoptotic potential of glucomoringin isothiocyanate (GMG-ITC) isolated from Moringa oleifera Lam seeds on human prostate cancer cells (PC-3) Abd Karim, Nurul Ashikin Adam, Aziza Hussein Bakheit Jaafaru, Mohammed Sani Rukayadi, Yaya Abdull Razis, Ahmad Faizal Inhibition of several protein pathways involved in cancer cell regulation is a necessary key in the discovery of cancer chemotherapy. Moringa oleifera Lam is often used in traditional medicine for the treatment of various illnesses. The plant contains glucomoringin isothiocyanate (GMG-ITC) with therapeutic potential against various cancer cells. Therefore, GMG-ITC was evaluated for its cytotoxicity against the PC-3 prostate cancer cell line and its potential to induce apoptosis. GMG-ITC inhibited cell proliferation in the PC-3 cell line with IC50 value 3.5 µg/mL. Morphological changes as a result of GMG-ITC-induced apoptosis showed chromatin condensation, nuclear fragmentation, and membrane blebbing. Additionally, Annexin V assay showed proportion of cells in early and late apoptosis upon exposure to GMG-ITC in a time-dependent manner. Moreover, GMG-ITC induced a time-dependent G2/M phase arrest, with reduction of 39.1 in the PC-3 cell line. GMG-ITC also activates apoptotic genes including caspase, tumor suppressor gene (p53), Akt/MAPK, and Bax of the proapoptotic Bcl family. Early apoptosis proteins (JNK, Bad, Bcl2, and p53) were significantly upregulated upon GMG-ITC treatment. It is concluded that apoptosis induction was observed in PC-3 cells treated with GMG-ITC. These phenomena suggest that GMG-ITC from M. oleifera seeds could be useful as a future cytotoxic agent against prostate cancer. Multidisciplinary Digital Publishing Institute 2023-04-04 Article PeerReviewed Abd Karim, Nurul Ashikin and Adam, Aziza Hussein Bakheit and Jaafaru, Mohammed Sani and Rukayadi, Yaya and Abdull Razis, Ahmad Faizal (2023) Apoptotic potential of glucomoringin isothiocyanate (GMG-ITC) isolated from Moringa oleifera Lam seeds on human prostate cancer cells (PC-3). Molecules, 28 (7). art. no. 3214. pp. 1-18. ISSN 1431-5165; ESSN: 1420-3049 https://www.mdpi.com/1420-3049/28/7/3214 10.3390/molecules28073214 |
| spellingShingle | Abd Karim, Nurul Ashikin Adam, Aziza Hussein Bakheit Jaafaru, Mohammed Sani Rukayadi, Yaya Abdull Razis, Ahmad Faizal Apoptotic potential of glucomoringin isothiocyanate (GMG-ITC) isolated from Moringa oleifera Lam seeds on human prostate cancer cells (PC-3) |
| title | Apoptotic potential of glucomoringin isothiocyanate (GMG-ITC) isolated from Moringa oleifera Lam seeds on human prostate cancer cells (PC-3) |
| title_full | Apoptotic potential of glucomoringin isothiocyanate (GMG-ITC) isolated from Moringa oleifera Lam seeds on human prostate cancer cells (PC-3) |
| title_fullStr | Apoptotic potential of glucomoringin isothiocyanate (GMG-ITC) isolated from Moringa oleifera Lam seeds on human prostate cancer cells (PC-3) |
| title_full_unstemmed | Apoptotic potential of glucomoringin isothiocyanate (GMG-ITC) isolated from Moringa oleifera Lam seeds on human prostate cancer cells (PC-3) |
| title_short | Apoptotic potential of glucomoringin isothiocyanate (GMG-ITC) isolated from Moringa oleifera Lam seeds on human prostate cancer cells (PC-3) |
| title_sort | apoptotic potential of glucomoringin isothiocyanate (gmg-itc) isolated from moringa oleifera lam seeds on human prostate cancer cells (pc-3) |
| url | http://psasir.upm.edu.my/id/eprint/106635/ http://psasir.upm.edu.my/id/eprint/106635/ http://psasir.upm.edu.my/id/eprint/106635/ |