Antibacterial and wound healing activity of 2% formulation of 2-Medpy-3-CN on infected burn wounded animal model
Introduction: Humans have learned to recognize and process plants into medicinal forms through centuries. Burns can spread to other tissues, especially when infected with bacteria such as Methicillin-Resistant Staphylococcus aureus (MRSA). The study aimed to assess the in vivo antibacterial and woun...
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| Format: | Article |
| Language: | English |
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Universiti Putra Malaysia
2023
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| Online Access: | http://psasir.upm.edu.my/id/eprint/106605/ http://psasir.upm.edu.my/id/eprint/106605/1/Antibacterial%20and%20Wound%20Healing.pdf |
| _version_ | 1848864789785214976 |
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| author | Neela, Vasantha Kumari Ahmad, Nor Maziah Wada, Surajo Adamu Karunanidhi, Arun Kumar |
| author_facet | Neela, Vasantha Kumari Ahmad, Nor Maziah Wada, Surajo Adamu Karunanidhi, Arun Kumar |
| author_sort | Neela, Vasantha Kumari |
| building | UPM Institutional Repository |
| collection | Online Access |
| description | Introduction: Humans have learned to recognize and process plants into medicinal forms through centuries. Burns can spread to other tissues, especially when infected with bacteria such as Methicillin-Resistant Staphylococcus aureus (MRSA). The study aimed to assess the in vivo antibacterial and wound healing activity of 2% formulation of 2-Medpy-3-CN on infected burn wounded animal model. Methods: In vitro antibacterial activity of the Alsti was done by broth dilution and disc diffusion methods. Alsti 2% ointment was prepared for the infected burn wound treatment. A total of 18 rats are grouped into A, B, C, and D, the first three groups (A-C) were injured thermally, and Group D was used as healthy controls. The three test Groups were exposed to MRSA ATCC 43300 at 105 CFU/mL. Group A was treated with 2% Alsti, Group B with Silver sulfadiazine 1% (SSD), and Group C was untreated. Wounds healing was assessed by the healed area and microscopic identification of hematoxylin and eosin (H&E)-stained skin tissue. Results: Wound healing progresses with application of Alsti 2% ointment as observed through wound diameter and histopathological changes of the skin. Wound diameter decreases with treatments, while the contrary was observed in the non-treated group. Microscopic observation of the stained skin showed that epidermal development, and collagen formation progress with treatment days. Untreated wounds showed marked inflammation, progressive ulceration, and necrosis. Conclusion: Alsti 2% formulation showed antibacterial and wound healing activities, hence, can be used as alternative in burn wound infections. |
| first_indexed | 2025-11-15T13:54:24Z |
| format | Article |
| id | upm-106605 |
| institution | Universiti Putra Malaysia |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-15T13:54:24Z |
| publishDate | 2023 |
| publisher | Universiti Putra Malaysia |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | upm-1066052024-10-11T07:47:43Z http://psasir.upm.edu.my/id/eprint/106605/ Antibacterial and wound healing activity of 2% formulation of 2-Medpy-3-CN on infected burn wounded animal model Neela, Vasantha Kumari Ahmad, Nor Maziah Wada, Surajo Adamu Karunanidhi, Arun Kumar Introduction: Humans have learned to recognize and process plants into medicinal forms through centuries. Burns can spread to other tissues, especially when infected with bacteria such as Methicillin-Resistant Staphylococcus aureus (MRSA). The study aimed to assess the in vivo antibacterial and wound healing activity of 2% formulation of 2-Medpy-3-CN on infected burn wounded animal model. Methods: In vitro antibacterial activity of the Alsti was done by broth dilution and disc diffusion methods. Alsti 2% ointment was prepared for the infected burn wound treatment. A total of 18 rats are grouped into A, B, C, and D, the first three groups (A-C) were injured thermally, and Group D was used as healthy controls. The three test Groups were exposed to MRSA ATCC 43300 at 105 CFU/mL. Group A was treated with 2% Alsti, Group B with Silver sulfadiazine 1% (SSD), and Group C was untreated. Wounds healing was assessed by the healed area and microscopic identification of hematoxylin and eosin (H&E)-stained skin tissue. Results: Wound healing progresses with application of Alsti 2% ointment as observed through wound diameter and histopathological changes of the skin. Wound diameter decreases with treatments, while the contrary was observed in the non-treated group. Microscopic observation of the stained skin showed that epidermal development, and collagen formation progress with treatment days. Untreated wounds showed marked inflammation, progressive ulceration, and necrosis. Conclusion: Alsti 2% formulation showed antibacterial and wound healing activities, hence, can be used as alternative in burn wound infections. Universiti Putra Malaysia 2023 Article PeerReviewed text en http://psasir.upm.edu.my/id/eprint/106605/1/Antibacterial%20and%20Wound%20Healing.pdf Neela, Vasantha Kumari and Ahmad, Nor Maziah and Wada, Surajo Adamu and Karunanidhi, Arun Kumar (2023) Antibacterial and wound healing activity of 2% formulation of 2-Medpy-3-CN on infected burn wounded animal model. Malaysian Journal of Medicine and Health Sciences, 19 (3). pp. 5-12. ISSN 1675-8544; ESSN: 2636-9346 https://medic.upm.edu.my/upload/dokumen/2023051610430101_MJMHS_1063.pdf 10.47836/mjmhs.19.3.2 |
| spellingShingle | Neela, Vasantha Kumari Ahmad, Nor Maziah Wada, Surajo Adamu Karunanidhi, Arun Kumar Antibacterial and wound healing activity of 2% formulation of 2-Medpy-3-CN on infected burn wounded animal model |
| title | Antibacterial and wound healing activity of 2% formulation of 2-Medpy-3-CN on infected burn wounded animal model |
| title_full | Antibacterial and wound healing activity of 2% formulation of 2-Medpy-3-CN on infected burn wounded animal model |
| title_fullStr | Antibacterial and wound healing activity of 2% formulation of 2-Medpy-3-CN on infected burn wounded animal model |
| title_full_unstemmed | Antibacterial and wound healing activity of 2% formulation of 2-Medpy-3-CN on infected burn wounded animal model |
| title_short | Antibacterial and wound healing activity of 2% formulation of 2-Medpy-3-CN on infected burn wounded animal model |
| title_sort | antibacterial and wound healing activity of 2% formulation of 2-medpy-3-cn on infected burn wounded animal model |
| url | http://psasir.upm.edu.my/id/eprint/106605/ http://psasir.upm.edu.my/id/eprint/106605/ http://psasir.upm.edu.my/id/eprint/106605/ http://psasir.upm.edu.my/id/eprint/106605/1/Antibacterial%20and%20Wound%20Healing.pdf |