Analysis of trehalulose in kelulut honey samples via HPLC-MS

Over recent years, there had been many studies by Malaysian researchers focusing on kelulut (stingless bee) honey chemical profile. One of the ultimate purposes of carrying out the chemical profiling activity was to search for suitable unique biomarkers that can be used as quality as well as authent...

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Main Authors: J., Arif Zaidi, Zawawi, N., Ramlan, N. A. F. M., S., Nor Fadhilah
Format: Article
Published: Rynnye Lyan Resources 2023
Online Access:http://psasir.upm.edu.my/id/eprint/106520/
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author J., Arif Zaidi
Zawawi, N.
Ramlan, N. A. F. M.
S., Nor Fadhilah
author_facet J., Arif Zaidi
Zawawi, N.
Ramlan, N. A. F. M.
S., Nor Fadhilah
author_sort J., Arif Zaidi
building UPM Institutional Repository
collection Online Access
description Over recent years, there had been many studies by Malaysian researchers focusing on kelulut (stingless bee) honey chemical profile. One of the ultimate purposes of carrying out the chemical profiling activity was to search for suitable unique biomarkers that can be used as quality as well as authenticity indicators. Both aspects are crucial in order to maintain the premium status of kelulut honey as it has always been regarded as one of the best multifunctional local health supplements. An interesting finding on a chemical component of kelulut honey was recently published. Trehalulose was identified as a major component in kelulut honey sourced from both Geniotrigona thoracica and Heterotrigona itama which are common species in Malaysian stingless bee farms. Since trehalulose is not commonly available from other natural sources, it can be a good quality marker candidate. To qualify as a good quality marker, a biochemical component must fulfil some criteria. This article reports on the potential of trehalulose as a quality indicator or marker in H. itama honey. Our preliminary studies focused on the analytical aspect of trehalulose based on analysis using high-performance liquid chromatography (HPLC) with evaporative light scattering detection (ELSD). Our findings showed that all kelulut honey samples collected contained trehalulose and the HPLC analysis can be carried out using a simple method provided that a pure commercial standard is available.
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institution Universiti Putra Malaysia
institution_category Local University
last_indexed 2025-11-15T13:54:11Z
publishDate 2023
publisher Rynnye Lyan Resources
recordtype eprints
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spelling upm-1065202024-08-06T03:05:31Z http://psasir.upm.edu.my/id/eprint/106520/ Analysis of trehalulose in kelulut honey samples via HPLC-MS J., Arif Zaidi Zawawi, N. Ramlan, N. A. F. M. S., Nor Fadhilah Over recent years, there had been many studies by Malaysian researchers focusing on kelulut (stingless bee) honey chemical profile. One of the ultimate purposes of carrying out the chemical profiling activity was to search for suitable unique biomarkers that can be used as quality as well as authenticity indicators. Both aspects are crucial in order to maintain the premium status of kelulut honey as it has always been regarded as one of the best multifunctional local health supplements. An interesting finding on a chemical component of kelulut honey was recently published. Trehalulose was identified as a major component in kelulut honey sourced from both Geniotrigona thoracica and Heterotrigona itama which are common species in Malaysian stingless bee farms. Since trehalulose is not commonly available from other natural sources, it can be a good quality marker candidate. To qualify as a good quality marker, a biochemical component must fulfil some criteria. This article reports on the potential of trehalulose as a quality indicator or marker in H. itama honey. Our preliminary studies focused on the analytical aspect of trehalulose based on analysis using high-performance liquid chromatography (HPLC) with evaporative light scattering detection (ELSD). Our findings showed that all kelulut honey samples collected contained trehalulose and the HPLC analysis can be carried out using a simple method provided that a pure commercial standard is available. Rynnye Lyan Resources 2023 Article PeerReviewed J., Arif Zaidi and Zawawi, N. and Ramlan, N. A. F. M. and S., Nor Fadhilah (2023) Analysis of trehalulose in kelulut honey samples via HPLC-MS. Food Research, 6 (Suppl.2). pp. 278-283. ISSN 2550-2166 https://www.myfoodresearch.com/vol-69474supplementary-2.html 10.26656/fr.2017.6(s2).36
spellingShingle J., Arif Zaidi
Zawawi, N.
Ramlan, N. A. F. M.
S., Nor Fadhilah
Analysis of trehalulose in kelulut honey samples via HPLC-MS
title Analysis of trehalulose in kelulut honey samples via HPLC-MS
title_full Analysis of trehalulose in kelulut honey samples via HPLC-MS
title_fullStr Analysis of trehalulose in kelulut honey samples via HPLC-MS
title_full_unstemmed Analysis of trehalulose in kelulut honey samples via HPLC-MS
title_short Analysis of trehalulose in kelulut honey samples via HPLC-MS
title_sort analysis of trehalulose in kelulut honey samples via hplc-ms
url http://psasir.upm.edu.my/id/eprint/106520/
http://psasir.upm.edu.my/id/eprint/106520/
http://psasir.upm.edu.my/id/eprint/106520/