Autophagy inhibition suppresses Newcastle disease virus-induced cell death by inhibiting viral replication in human breast cancer cells
Newcastle disease virus (NDV) is an oncolytic virus which selectively replicates in cancer cells without harming normal cells. Autophagy is a cellular mechanism that breaks down unused cytoplasmic constituents into nutrients. In previous studies, autophagy enhanced NDV-induced oncolysis in lung canc...
| Main Authors: | , , , |
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| Format: | Article |
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Academic Press Inc.
2024
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| Online Access: | http://psasir.upm.edu.my/id/eprint/105859/ |
| _version_ | 1848864627354501120 |
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| author | Rozilah, Megat Irfan Yusoff, Khatijah Chia, Suet Lin Ismail, Saila |
| author_facet | Rozilah, Megat Irfan Yusoff, Khatijah Chia, Suet Lin Ismail, Saila |
| author_sort | Rozilah, Megat Irfan |
| building | UPM Institutional Repository |
| collection | Online Access |
| description | Newcastle disease virus (NDV) is an oncolytic virus which selectively replicates in cancer cells without harming normal cells. Autophagy is a cellular mechanism that breaks down unused cytoplasmic constituents into nutrients. In previous studies, autophagy enhanced NDV-induced oncolysis in lung cancer and glioma cells. However, the effect of autophagy inhibition on NDV-induced oncolysis in breast cancer cells remains unknown. This study aimed to examine the effect of autophagy inhibition on NDV-induced oncolysis in human breast cancer cells, MCF7. To inhibit autophagy, we knocked down the expression of the autophagy protein beclin-1 (BECN1) by short interfering RNA (siRNA). The cells were infected with the recombinant NDV strain AF2240 expressing green fluorescent protein. We found that NDV induced autophagy and knockdown of BECN1 significantly reduced the NDV-induced autophagy in MCF7 cells. Importantly, BECN1 knockdown significantly suppressed cell death by inhibiting viral replication, as observed at 24 h post infection. Overall, our data suggest that autophagy inhibition may not be a suitable strategy to enhance NDV oncolytic efficacy against breast cancer. |
| first_indexed | 2025-11-15T13:51:49Z |
| format | Article |
| id | upm-105859 |
| institution | Universiti Putra Malaysia |
| institution_category | Local University |
| last_indexed | 2025-11-15T13:51:49Z |
| publishDate | 2024 |
| publisher | Academic Press Inc. |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | upm-1058592024-03-27T01:10:05Z http://psasir.upm.edu.my/id/eprint/105859/ Autophagy inhibition suppresses Newcastle disease virus-induced cell death by inhibiting viral replication in human breast cancer cells Rozilah, Megat Irfan Yusoff, Khatijah Chia, Suet Lin Ismail, Saila Newcastle disease virus (NDV) is an oncolytic virus which selectively replicates in cancer cells without harming normal cells. Autophagy is a cellular mechanism that breaks down unused cytoplasmic constituents into nutrients. In previous studies, autophagy enhanced NDV-induced oncolysis in lung cancer and glioma cells. However, the effect of autophagy inhibition on NDV-induced oncolysis in breast cancer cells remains unknown. This study aimed to examine the effect of autophagy inhibition on NDV-induced oncolysis in human breast cancer cells, MCF7. To inhibit autophagy, we knocked down the expression of the autophagy protein beclin-1 (BECN1) by short interfering RNA (siRNA). The cells were infected with the recombinant NDV strain AF2240 expressing green fluorescent protein. We found that NDV induced autophagy and knockdown of BECN1 significantly reduced the NDV-induced autophagy in MCF7 cells. Importantly, BECN1 knockdown significantly suppressed cell death by inhibiting viral replication, as observed at 24 h post infection. Overall, our data suggest that autophagy inhibition may not be a suitable strategy to enhance NDV oncolytic efficacy against breast cancer. Academic Press Inc. 2024 Article PeerReviewed Rozilah, Megat Irfan and Yusoff, Khatijah and Chia, Suet Lin and Ismail, Saila (2024) Autophagy inhibition suppresses Newcastle disease virus-induced cell death by inhibiting viral replication in human breast cancer cells. Virology, 590. art. no. 109957. pp. 1-7. ISSN 0042-6822; ESSN: 1096-0341 https://www.sciencedirect.com/science/article/pii/S0042682223002763 10.1016/j.virol.2023.109957 |
| spellingShingle | Rozilah, Megat Irfan Yusoff, Khatijah Chia, Suet Lin Ismail, Saila Autophagy inhibition suppresses Newcastle disease virus-induced cell death by inhibiting viral replication in human breast cancer cells |
| title | Autophagy inhibition suppresses Newcastle disease virus-induced cell death by inhibiting viral replication in human breast cancer cells |
| title_full | Autophagy inhibition suppresses Newcastle disease virus-induced cell death by inhibiting viral replication in human breast cancer cells |
| title_fullStr | Autophagy inhibition suppresses Newcastle disease virus-induced cell death by inhibiting viral replication in human breast cancer cells |
| title_full_unstemmed | Autophagy inhibition suppresses Newcastle disease virus-induced cell death by inhibiting viral replication in human breast cancer cells |
| title_short | Autophagy inhibition suppresses Newcastle disease virus-induced cell death by inhibiting viral replication in human breast cancer cells |
| title_sort | autophagy inhibition suppresses newcastle disease virus-induced cell death by inhibiting viral replication in human breast cancer cells |
| url | http://psasir.upm.edu.my/id/eprint/105859/ http://psasir.upm.edu.my/id/eprint/105859/ http://psasir.upm.edu.my/id/eprint/105859/ |