Nanocomposites of iron oxide, sodium alginate, and eugenol induce apoptosis via PI3K/Akt/mTOR signaling in Hep3 cells and in vivo hepatotoxicity in the zebrafish model
Hepatic cancer is among the most recurrently detected malignancies worldwide and one of the main contributors to cancer-associated mortality. With few available therapeutic choices, there is an instant necessity to explore suitable options. In this aspect, Nanotechnology has been employed to explore...
| Main Authors: | , , , , , , , , , , , , , |
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| Format: | Article |
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Elsevier B.V.
2024
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| Online Access: | http://psasir.upm.edu.my/id/eprint/105814/ |
| _version_ | 1848864614972915712 |
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| author | Elderdery, Abozer Y. Alzerwi, Nasser A.N. Alzahrani, Badr Alsrhani, Abdullah Alsultan, Afnan Rayzah, Musaed Idrees, Bandar Rayzah, Fares Baksh, Yaser Alzahrani, Ahmed M. Alabdulsalam, Abdulrahim A. Mohamedain, A. Subbiah, Suresh Kumar Mok, Pooi Ling |
| author_facet | Elderdery, Abozer Y. Alzerwi, Nasser A.N. Alzahrani, Badr Alsrhani, Abdullah Alsultan, Afnan Rayzah, Musaed Idrees, Bandar Rayzah, Fares Baksh, Yaser Alzahrani, Ahmed M. Alabdulsalam, Abdulrahim A. Mohamedain, A. Subbiah, Suresh Kumar Mok, Pooi Ling |
| author_sort | Elderdery, Abozer Y. |
| building | UPM Institutional Repository |
| collection | Online Access |
| description | Hepatic cancer is among the most recurrently detected malignancies worldwide and one of the main contributors to cancer-associated mortality. With few available therapeutic choices, there is an instant necessity to explore suitable options. In this aspect, Nanotechnology has been employed to explore prospective chemotherapeutic approaches, especially for cancer treatment. Nanotechnology is concerned with the biological and physical properties of nanoparticles in the therapeutic use of drugs. In the current work, formulation, and characterization of α-Fe2O3–Sodium Alginate-Eugenol nanocomposites (FSE NCs) using several approaches like SEM and TEM, UV–visible, FTIR, and PL spectroscopy, XRD, EDAX, and DLS studies have been performed. With an average size of 50 nm, the rhombohedral structure of NCs was identified. Further, their anticancer activity against Hep3B liver cancer cell lines has been performed by cell viability, dual staining, DCFH-DA, Annexin-V/-FITC/PI, cell cycle analysis methods, and PI3K/Akt/mTOR signaling proteins were studied to assess the anticancer effects of the NCs in Hep3B cells. Also, anti-cancer activity on animal modeling in-vivo using zebra fishes to hematological parameters, liver enzymes, and histopathology study effectiveness was noticed. Moreover, the NCs reduced the viability, elevated the ROS accumulation, diminished the membrane integrity, reduced the antioxidants, blocked the cell cycle, and triggered the PI3K/Akt/mTOR signaling axis that eventually resulted in cell death. As a result, FSE NCs possess huge potential for use as a possible anticancer candidate. |
| first_indexed | 2025-11-15T13:51:37Z |
| format | Article |
| id | upm-105814 |
| institution | Universiti Putra Malaysia |
| institution_category | Local University |
| last_indexed | 2025-11-15T13:51:37Z |
| publishDate | 2024 |
| publisher | Elsevier B.V. |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | upm-1058142024-04-04T07:56:48Z http://psasir.upm.edu.my/id/eprint/105814/ Nanocomposites of iron oxide, sodium alginate, and eugenol induce apoptosis via PI3K/Akt/mTOR signaling in Hep3 cells and in vivo hepatotoxicity in the zebrafish model Elderdery, Abozer Y. Alzerwi, Nasser A.N. Alzahrani, Badr Alsrhani, Abdullah Alsultan, Afnan Rayzah, Musaed Idrees, Bandar Rayzah, Fares Baksh, Yaser Alzahrani, Ahmed M. Alabdulsalam, Abdulrahim A. Mohamedain, A. Subbiah, Suresh Kumar Mok, Pooi Ling Hepatic cancer is among the most recurrently detected malignancies worldwide and one of the main contributors to cancer-associated mortality. With few available therapeutic choices, there is an instant necessity to explore suitable options. In this aspect, Nanotechnology has been employed to explore prospective chemotherapeutic approaches, especially for cancer treatment. Nanotechnology is concerned with the biological and physical properties of nanoparticles in the therapeutic use of drugs. In the current work, formulation, and characterization of α-Fe2O3–Sodium Alginate-Eugenol nanocomposites (FSE NCs) using several approaches like SEM and TEM, UV–visible, FTIR, and PL spectroscopy, XRD, EDAX, and DLS studies have been performed. With an average size of 50 nm, the rhombohedral structure of NCs was identified. Further, their anticancer activity against Hep3B liver cancer cell lines has been performed by cell viability, dual staining, DCFH-DA, Annexin-V/-FITC/PI, cell cycle analysis methods, and PI3K/Akt/mTOR signaling proteins were studied to assess the anticancer effects of the NCs in Hep3B cells. Also, anti-cancer activity on animal modeling in-vivo using zebra fishes to hematological parameters, liver enzymes, and histopathology study effectiveness was noticed. Moreover, the NCs reduced the viability, elevated the ROS accumulation, diminished the membrane integrity, reduced the antioxidants, blocked the cell cycle, and triggered the PI3K/Akt/mTOR signaling axis that eventually resulted in cell death. As a result, FSE NCs possess huge potential for use as a possible anticancer candidate. Elsevier B.V. 2024 Article PeerReviewed Elderdery, Abozer Y. and Alzerwi, Nasser A.N. and Alzahrani, Badr and Alsrhani, Abdullah and Alsultan, Afnan and Rayzah, Musaed and Idrees, Bandar and Rayzah, Fares and Baksh, Yaser and Alzahrani, Ahmed M. and Alabdulsalam, Abdulrahim A. and Mohamedain, A. and Subbiah, Suresh Kumar and Mok, Pooi Ling (2024) Nanocomposites of iron oxide, sodium alginate, and eugenol induce apoptosis via PI3K/Akt/mTOR signaling in Hep3 cells and in vivo hepatotoxicity in the zebrafish model. International Journal of Biological Macromolecules, 256 (pt.2). art. no. 127490. pp. 1-15. ISSN 0141-8130; ESSN: 1879-0003 https://www.sciencedirect.com/science/article/pii/S0141813023043878 10.1016/j.ijbiomac.2023.127490 |
| spellingShingle | Elderdery, Abozer Y. Alzerwi, Nasser A.N. Alzahrani, Badr Alsrhani, Abdullah Alsultan, Afnan Rayzah, Musaed Idrees, Bandar Rayzah, Fares Baksh, Yaser Alzahrani, Ahmed M. Alabdulsalam, Abdulrahim A. Mohamedain, A. Subbiah, Suresh Kumar Mok, Pooi Ling Nanocomposites of iron oxide, sodium alginate, and eugenol induce apoptosis via PI3K/Akt/mTOR signaling in Hep3 cells and in vivo hepatotoxicity in the zebrafish model |
| title | Nanocomposites of iron oxide, sodium alginate, and eugenol induce apoptosis via PI3K/Akt/mTOR signaling in Hep3 cells and in vivo hepatotoxicity in the zebrafish model |
| title_full | Nanocomposites of iron oxide, sodium alginate, and eugenol induce apoptosis via PI3K/Akt/mTOR signaling in Hep3 cells and in vivo hepatotoxicity in the zebrafish model |
| title_fullStr | Nanocomposites of iron oxide, sodium alginate, and eugenol induce apoptosis via PI3K/Akt/mTOR signaling in Hep3 cells and in vivo hepatotoxicity in the zebrafish model |
| title_full_unstemmed | Nanocomposites of iron oxide, sodium alginate, and eugenol induce apoptosis via PI3K/Akt/mTOR signaling in Hep3 cells and in vivo hepatotoxicity in the zebrafish model |
| title_short | Nanocomposites of iron oxide, sodium alginate, and eugenol induce apoptosis via PI3K/Akt/mTOR signaling in Hep3 cells and in vivo hepatotoxicity in the zebrafish model |
| title_sort | nanocomposites of iron oxide, sodium alginate, and eugenol induce apoptosis via pi3k/akt/mtor signaling in hep3 cells and in vivo hepatotoxicity in the zebrafish model |
| url | http://psasir.upm.edu.my/id/eprint/105814/ http://psasir.upm.edu.my/id/eprint/105814/ http://psasir.upm.edu.my/id/eprint/105814/ |