Development of novel biofilm using Musa acuminata (waste banana leaves) mediated biogenic zinc oxide nanoparticles reinforced with chitosan blend
Objectives: The present study reports a cost-effective, eco-friendly, and straightforward approach to synthesize Zinc Oxide Nanoparticles (ZnO NPs) using agro-waste banana (Musa acuminata) leaves and compares it with chemogenic ZnO NP. Methods: The study investigated biogenic and chemogenic ZnO NPs,...
| Main Authors: | , , , , , , |
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| Format: | Article |
| Language: | English |
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Elsevier
2024
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| Online Access: | http://psasir.upm.edu.my/id/eprint/105682/ http://psasir.upm.edu.my/id/eprint/105682/1/1-s2.0-S1018364723005426-main.pdf |
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| author | Sasidharan, Shivitraloshini Tey, Lai-Hock Djearamane, Sinouvassane Mahmud Ab Rashid, Nor Khaizura PA, Raajeswari Shing Wong, Ling Tanislaus Antony Dhanapal, Anto Cordelia |
| author_facet | Sasidharan, Shivitraloshini Tey, Lai-Hock Djearamane, Sinouvassane Mahmud Ab Rashid, Nor Khaizura PA, Raajeswari Shing Wong, Ling Tanislaus Antony Dhanapal, Anto Cordelia |
| author_sort | Sasidharan, Shivitraloshini |
| building | UPM Institutional Repository |
| collection | Online Access |
| description | Objectives: The present study reports a cost-effective, eco-friendly, and straightforward approach to synthesize Zinc Oxide Nanoparticles (ZnO NPs) using agro-waste banana (Musa acuminata) leaves and compares it with chemogenic ZnO NP. Methods: The study investigated biogenic and chemogenic ZnO NPs, examining their size, shape, morphological structure, and stability using UV–Vis Spectroscopy, X-Ray Diffraction (XRD), Field Transmission Scanning Electron Microscopy (FESEM), Energy Dispersive X-Ray (EDX), and Fourier Transform Infrared Spectroscopy (FTIR). Antibacterial potency against Staphylococcus aureus and Salmonella typhimurium was tested through the Disk Diffusion method. Bio-nanocomposites were created by incorporating different ZnO NP concentrations into chitosan via the solvent casting method. Film characteristics were analyzed using SEM, tensile strength, film moisture (FM), film solubility (FS), and water-holding capacity (WHC). Antibacterial activity of the bio-nanocomposites was assessed against Staphylococcus aureus and Salmonella typhimurium using the Viable Cell Colony Count method. Results: The results of color change confirmed the formation of biogenic and chemogenic ZnO NP having sharp peaks at 370 nm and 381 nm respectively having spherical structure with an average crystalline size of 36.73 nm and 75.31 nm. The biogenic ZnO NP had greater inhibitory action against both Staphylococcus aureus and Salmonella typhimurium than chemogenic ZnO NP. Besides, the biogenic biofilms had enhanced characteristics followed by chemogenic and chitosan biofilms. Conclusion: The study suggests a green approach, using smaller nanosized biogenic ZnO NPs with unique advantages over chemogenic counterparts. The resulting biofilms exhibit the potential to enhance food quality, safety, and longevity in the food industry. This aligns with circular green economy principles, utilizing waste banana leaves for raw material synthesis and promoting manufacturing innovation. |
| first_indexed | 2025-11-15T13:51:03Z |
| format | Article |
| id | upm-105682 |
| institution | Universiti Putra Malaysia |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-15T13:51:03Z |
| publishDate | 2024 |
| publisher | Elsevier |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | upm-1056822024-07-10T05:21:43Z http://psasir.upm.edu.my/id/eprint/105682/ Development of novel biofilm using Musa acuminata (waste banana leaves) mediated biogenic zinc oxide nanoparticles reinforced with chitosan blend Sasidharan, Shivitraloshini Tey, Lai-Hock Djearamane, Sinouvassane Mahmud Ab Rashid, Nor Khaizura PA, Raajeswari Shing Wong, Ling Tanislaus Antony Dhanapal, Anto Cordelia Objectives: The present study reports a cost-effective, eco-friendly, and straightforward approach to synthesize Zinc Oxide Nanoparticles (ZnO NPs) using agro-waste banana (Musa acuminata) leaves and compares it with chemogenic ZnO NP. Methods: The study investigated biogenic and chemogenic ZnO NPs, examining their size, shape, morphological structure, and stability using UV–Vis Spectroscopy, X-Ray Diffraction (XRD), Field Transmission Scanning Electron Microscopy (FESEM), Energy Dispersive X-Ray (EDX), and Fourier Transform Infrared Spectroscopy (FTIR). Antibacterial potency against Staphylococcus aureus and Salmonella typhimurium was tested through the Disk Diffusion method. Bio-nanocomposites were created by incorporating different ZnO NP concentrations into chitosan via the solvent casting method. Film characteristics were analyzed using SEM, tensile strength, film moisture (FM), film solubility (FS), and water-holding capacity (WHC). Antibacterial activity of the bio-nanocomposites was assessed against Staphylococcus aureus and Salmonella typhimurium using the Viable Cell Colony Count method. Results: The results of color change confirmed the formation of biogenic and chemogenic ZnO NP having sharp peaks at 370 nm and 381 nm respectively having spherical structure with an average crystalline size of 36.73 nm and 75.31 nm. The biogenic ZnO NP had greater inhibitory action against both Staphylococcus aureus and Salmonella typhimurium than chemogenic ZnO NP. Besides, the biogenic biofilms had enhanced characteristics followed by chemogenic and chitosan biofilms. Conclusion: The study suggests a green approach, using smaller nanosized biogenic ZnO NPs with unique advantages over chemogenic counterparts. The resulting biofilms exhibit the potential to enhance food quality, safety, and longevity in the food industry. This aligns with circular green economy principles, utilizing waste banana leaves for raw material synthesis and promoting manufacturing innovation. Elsevier 2024 Article PeerReviewed text en http://psasir.upm.edu.my/id/eprint/105682/1/1-s2.0-S1018364723005426-main.pdf Sasidharan, Shivitraloshini and Tey, Lai-Hock and Djearamane, Sinouvassane and Mahmud Ab Rashid, Nor Khaizura and PA, Raajeswari and Shing Wong, Ling and Tanislaus Antony Dhanapal, Anto Cordelia (2024) Development of novel biofilm using Musa acuminata (waste banana leaves) mediated biogenic zinc oxide nanoparticles reinforced with chitosan blend. Journal of King Saud University - Science, 36 (3). art. no. 103080. pp. 1-11. ISSN 1018-3647 https://www.sciencedirect.com/science/article/pii/S1018364723005426?via%3Dihub 10.1016/j.jksus.2023.103080 |
| spellingShingle | Sasidharan, Shivitraloshini Tey, Lai-Hock Djearamane, Sinouvassane Mahmud Ab Rashid, Nor Khaizura PA, Raajeswari Shing Wong, Ling Tanislaus Antony Dhanapal, Anto Cordelia Development of novel biofilm using Musa acuminata (waste banana leaves) mediated biogenic zinc oxide nanoparticles reinforced with chitosan blend |
| title | Development of novel biofilm using Musa acuminata (waste banana leaves) mediated biogenic zinc oxide nanoparticles reinforced with chitosan blend |
| title_full | Development of novel biofilm using Musa acuminata (waste banana leaves) mediated biogenic zinc oxide nanoparticles reinforced with chitosan blend |
| title_fullStr | Development of novel biofilm using Musa acuminata (waste banana leaves) mediated biogenic zinc oxide nanoparticles reinforced with chitosan blend |
| title_full_unstemmed | Development of novel biofilm using Musa acuminata (waste banana leaves) mediated biogenic zinc oxide nanoparticles reinforced with chitosan blend |
| title_short | Development of novel biofilm using Musa acuminata (waste banana leaves) mediated biogenic zinc oxide nanoparticles reinforced with chitosan blend |
| title_sort | development of novel biofilm using musa acuminata (waste banana leaves) mediated biogenic zinc oxide nanoparticles reinforced with chitosan blend |
| url | http://psasir.upm.edu.my/id/eprint/105682/ http://psasir.upm.edu.my/id/eprint/105682/ http://psasir.upm.edu.my/id/eprint/105682/ http://psasir.upm.edu.my/id/eprint/105682/1/1-s2.0-S1018364723005426-main.pdf |