Role of cell surface proteins and toll-like receptors in the pathogenesis of Streptococcus pneumoniae
Objective(s): Pneumococcal cell wall (PCW) is an inflammatory component in Streptococcus pneumoniae. The cell surface proteins and the toll-like receptors (TLR) signaling response were investigated in the human lung epithelial (A549) cells inoculated with PCW of different serotypes. Materials and Me...
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| Format: | Article |
| Language: | English |
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Mashhad University of Medical Sciences
2024
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| Online Access: | http://psasir.upm.edu.my/id/eprint/105679/ http://psasir.upm.edu.my/id/eprint/105679/1/IJBMS_Volume%2027_Issue%202_Pages%20214-222.pdf |
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| author | Anuar, Nurul Fathiyah Zaipul Desa, Mohd Nasir Mohd Hussaini, Jamal Wong, Eng Hwa Mariappan, Vanitha Vellasamy, Kumutha Malar Palanisamy, Navindra Kumari |
| author_facet | Anuar, Nurul Fathiyah Zaipul Desa, Mohd Nasir Mohd Hussaini, Jamal Wong, Eng Hwa Mariappan, Vanitha Vellasamy, Kumutha Malar Palanisamy, Navindra Kumari |
| author_sort | Anuar, Nurul Fathiyah Zaipul |
| building | UPM Institutional Repository |
| collection | Online Access |
| description | Objective(s): Pneumococcal cell wall (PCW) is an inflammatory component in Streptococcus pneumoniae. The cell surface proteins and the toll-like receptors (TLR) signaling response were investigated in the human lung epithelial (A549) cells inoculated with PCW of different serotypes. Materials and Methods: The presence of genes encoding these proteins was determined using polymerase chain reaction (PCR). The structure of the cell walls was analyzed by proton nuclear magnetic resonance (1H NMR). The A549 cell line was challenged with PCW extracts of different serotypes. RNA from the infected host cells was extracted and tested against a total of 84 genes associated with TLR signaling pathways (TLR 1-6 and 10) using RT2 Profiler PCR Array. Results: Cell surface proteins; ply, lytA, nanA, nanB, and cbpD genes were present in all serotypes. The distribution and structure of surface protein genes suggest behavioral changes in the molecules, contributing to the resilience of the strains to antibiotic treatment. Conclusion: TLR2 showed the highest expression, while serotypes 1, 3, and 5 induced higher TNFα and IL-1α, suggesting to be more immunogenic than the other strains tested. © 2024 Mashhad University of Medical Sciences. All rights reserved. |
| first_indexed | 2025-11-15T13:51:02Z |
| format | Article |
| id | upm-105679 |
| institution | Universiti Putra Malaysia |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-15T13:51:02Z |
| publishDate | 2024 |
| publisher | Mashhad University of Medical Sciences |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | upm-1056792024-07-10T05:16:58Z http://psasir.upm.edu.my/id/eprint/105679/ Role of cell surface proteins and toll-like receptors in the pathogenesis of Streptococcus pneumoniae Anuar, Nurul Fathiyah Zaipul Desa, Mohd Nasir Mohd Hussaini, Jamal Wong, Eng Hwa Mariappan, Vanitha Vellasamy, Kumutha Malar Palanisamy, Navindra Kumari Objective(s): Pneumococcal cell wall (PCW) is an inflammatory component in Streptococcus pneumoniae. The cell surface proteins and the toll-like receptors (TLR) signaling response were investigated in the human lung epithelial (A549) cells inoculated with PCW of different serotypes. Materials and Methods: The presence of genes encoding these proteins was determined using polymerase chain reaction (PCR). The structure of the cell walls was analyzed by proton nuclear magnetic resonance (1H NMR). The A549 cell line was challenged with PCW extracts of different serotypes. RNA from the infected host cells was extracted and tested against a total of 84 genes associated with TLR signaling pathways (TLR 1-6 and 10) using RT2 Profiler PCR Array. Results: Cell surface proteins; ply, lytA, nanA, nanB, and cbpD genes were present in all serotypes. The distribution and structure of surface protein genes suggest behavioral changes in the molecules, contributing to the resilience of the strains to antibiotic treatment. Conclusion: TLR2 showed the highest expression, while serotypes 1, 3, and 5 induced higher TNFα and IL-1α, suggesting to be more immunogenic than the other strains tested. © 2024 Mashhad University of Medical Sciences. All rights reserved. Mashhad University of Medical Sciences 2024 Article PeerReviewed text en http://psasir.upm.edu.my/id/eprint/105679/1/IJBMS_Volume%2027_Issue%202_Pages%20214-222.pdf Anuar, Nurul Fathiyah Zaipul and Desa, Mohd Nasir Mohd and Hussaini, Jamal and Wong, Eng Hwa and Mariappan, Vanitha and Vellasamy, Kumutha Malar and Palanisamy, Navindra Kumari (2024) Role of cell surface proteins and toll-like receptors in the pathogenesis of Streptococcus pneumoniae. Iranian Journal of Basic Medical Sciences, 27 (2). pp. 214-222. ISSN 2008-3866; ESSN: 2008-3874 https://ijbms.mums.ac.ir/article_23229.html 10.22038/IJBMS.2023.72584.15791 |
| spellingShingle | Anuar, Nurul Fathiyah Zaipul Desa, Mohd Nasir Mohd Hussaini, Jamal Wong, Eng Hwa Mariappan, Vanitha Vellasamy, Kumutha Malar Palanisamy, Navindra Kumari Role of cell surface proteins and toll-like receptors in the pathogenesis of Streptococcus pneumoniae |
| title | Role of cell surface proteins and toll-like receptors in the pathogenesis of Streptococcus pneumoniae |
| title_full | Role of cell surface proteins and toll-like receptors in the pathogenesis of Streptococcus pneumoniae |
| title_fullStr | Role of cell surface proteins and toll-like receptors in the pathogenesis of Streptococcus pneumoniae |
| title_full_unstemmed | Role of cell surface proteins and toll-like receptors in the pathogenesis of Streptococcus pneumoniae |
| title_short | Role of cell surface proteins and toll-like receptors in the pathogenesis of Streptococcus pneumoniae |
| title_sort | role of cell surface proteins and toll-like receptors in the pathogenesis of streptococcus pneumoniae |
| url | http://psasir.upm.edu.my/id/eprint/105679/ http://psasir.upm.edu.my/id/eprint/105679/ http://psasir.upm.edu.my/id/eprint/105679/ http://psasir.upm.edu.my/id/eprint/105679/1/IJBMS_Volume%2027_Issue%202_Pages%20214-222.pdf |