Thermostability engineering of industrial enzymes through structure modification
Thermostability is an essential requirement of enzymes in the industrial processes to catalyze the reactions at high temperatures; thus, enzyme engineering through directed evolution, semi-rational design and rational design are commonly employed to construct desired thermostable mutants. Several st...
| Main Authors: | , , , , , |
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| Format: | Article |
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Springer
2022
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| Online Access: | http://psasir.upm.edu.my/id/eprint/103719/ |
| _version_ | 1848864089184403456 |
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| author | Nezhad, Nima Ghahremani Raja Abd Rahman, Raja Noor Zaliha M. Normi, Yahaya Oslan, Siti Nurbaya Mohd Shariff, Fairolniza Leow, Adam Thean Chor |
| author_facet | Nezhad, Nima Ghahremani Raja Abd Rahman, Raja Noor Zaliha M. Normi, Yahaya Oslan, Siti Nurbaya Mohd Shariff, Fairolniza Leow, Adam Thean Chor |
| author_sort | Nezhad, Nima Ghahremani |
| building | UPM Institutional Repository |
| collection | Online Access |
| description | Thermostability is an essential requirement of enzymes in the industrial processes to catalyze the reactions at high temperatures; thus, enzyme engineering through directed evolution, semi-rational design and rational design are commonly employed to construct desired thermostable mutants. Several strategies are implemented to fulfill enzymes’ thermostability demand including decreasing the entropy of the unfolded state through substitutions Gly → Xxx or Xxx → Pro, hydrogen bond, salt bridge, introducing two different simultaneous interactions through single mutant, hydrophobic interaction, filling the hydrophobic cavity core, decreasing surface hydrophobicity, truncating loop, aromatic-aromatic interaction and introducing positively charged residues to enzyme surface. In the current review, horizons about compatibility between secondary structures and substitutions at preferable structural positions to generate the most desirable thermostability in industrial enzymes are broadened. |
| first_indexed | 2025-11-15T13:43:16Z |
| format | Article |
| id | upm-103719 |
| institution | Universiti Putra Malaysia |
| institution_category | Local University |
| last_indexed | 2025-11-15T13:43:16Z |
| publishDate | 2022 |
| publisher | Springer |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | upm-1037192023-04-14T07:47:27Z http://psasir.upm.edu.my/id/eprint/103719/ Thermostability engineering of industrial enzymes through structure modification Nezhad, Nima Ghahremani Raja Abd Rahman, Raja Noor Zaliha M. Normi, Yahaya Oslan, Siti Nurbaya Mohd Shariff, Fairolniza Leow, Adam Thean Chor Thermostability is an essential requirement of enzymes in the industrial processes to catalyze the reactions at high temperatures; thus, enzyme engineering through directed evolution, semi-rational design and rational design are commonly employed to construct desired thermostable mutants. Several strategies are implemented to fulfill enzymes’ thermostability demand including decreasing the entropy of the unfolded state through substitutions Gly → Xxx or Xxx → Pro, hydrogen bond, salt bridge, introducing two different simultaneous interactions through single mutant, hydrophobic interaction, filling the hydrophobic cavity core, decreasing surface hydrophobicity, truncating loop, aromatic-aromatic interaction and introducing positively charged residues to enzyme surface. In the current review, horizons about compatibility between secondary structures and substitutions at preferable structural positions to generate the most desirable thermostability in industrial enzymes are broadened. Springer 2022 Article PeerReviewed Nezhad, Nima Ghahremani and Raja Abd Rahman, Raja Noor Zaliha and M. Normi, Yahaya and Oslan, Siti Nurbaya and Mohd Shariff, Fairolniza and Leow, Adam Thean Chor (2022) Thermostability engineering of industrial enzymes through structure modification. Applied Microbiology and Biotechnology, 106. 4845 - 4866. ISSN 0175-7598; ESSN:1432-0614 https://link.springer.com/article/10.1007/s00253-022-12067-x |
| spellingShingle | Nezhad, Nima Ghahremani Raja Abd Rahman, Raja Noor Zaliha M. Normi, Yahaya Oslan, Siti Nurbaya Mohd Shariff, Fairolniza Leow, Adam Thean Chor Thermostability engineering of industrial enzymes through structure modification |
| title | Thermostability engineering of industrial enzymes through structure modification |
| title_full | Thermostability engineering of industrial enzymes through structure modification |
| title_fullStr | Thermostability engineering of industrial enzymes through structure modification |
| title_full_unstemmed | Thermostability engineering of industrial enzymes through structure modification |
| title_short | Thermostability engineering of industrial enzymes through structure modification |
| title_sort | thermostability engineering of industrial enzymes through structure modification |
| url | http://psasir.upm.edu.my/id/eprint/103719/ http://psasir.upm.edu.my/id/eprint/103719/ |