The effect of Aflatoxin B1 on tumor-related genes and phenotypic characters of MCF7 and MCF10A cells
The fungal toxin aflatoxin B1 (AB1) and its reactive intermediate, aflatoxin B1-8, 9 epoxide, could cause liver cancer by inducing DNA adducts. AB1 exposure can induce changes in the expression of several cancer-related genes. In this study, the effect of AB1 exposure on breast cancer MCF7 and norma...
| Main Authors: | , , , , , , , , , , , |
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| Format: | Article |
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Multidisciplinary Digital Publishing Institute
2022
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| Online Access: | http://psasir.upm.edu.my/id/eprint/103499/ |
| _version_ | 1848864034517942272 |
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| author | Ahmed Adam, Mowaffaq Adam Muhd Kamal, Laina Zarisa Kanakal, Mahibub Babu, Dinesh Dahham, Saad Sabbar Tabana, Yasser Lok, Bronwyn Bermoy, Brittany M. Yunus, Muhammad Amir Than, Leslie Thian Lung Barakat, Khaled Sandai, Doblin |
| author_facet | Ahmed Adam, Mowaffaq Adam Muhd Kamal, Laina Zarisa Kanakal, Mahibub Babu, Dinesh Dahham, Saad Sabbar Tabana, Yasser Lok, Bronwyn Bermoy, Brittany M. Yunus, Muhammad Amir Than, Leslie Thian Lung Barakat, Khaled Sandai, Doblin |
| author_sort | Ahmed Adam, Mowaffaq Adam |
| building | UPM Institutional Repository |
| collection | Online Access |
| description | The fungal toxin aflatoxin B1 (AB1) and its reactive intermediate, aflatoxin B1-8, 9 epoxide, could cause liver cancer by inducing DNA adducts. AB1 exposure can induce changes in the expression of several cancer-related genes. In this study, the effect of AB1 exposure on breast cancer MCF7 and normal breast MCF10A cell lines at the phenotypic and epigenetic levels was investigated to evaluate its potential in increasing the risk of breast cancer development. We hypothesized that, even at low concentrations, AB1 can cause changes in the expression of important genes involved in four pathways, i.e., p53, cancer, cell cycle, and apoptosis. The transcriptomic levels of BRCA1, BRCA2, p53, HER1, HER2, cMyc, BCL2, MCL1, CCND1, WNT3A, MAPK1, MAPK3, DAPK1, Casp8, and Casp9 were determined in MCF7 and MCF10A cells. Our results illustrate that treating both cells with AB1 induced cytotoxicity and apoptosis with reduction in cell viability in a concentration-dependent manner. Additionally, AB1 reduced reactive oxygen species levels. Phenotypically, AB1 caused cell-cycle arrest at G1, hypertrophy, and increased cell migration rates. There were changes in the expression levels of several tumor-related genes, which are known to contribute to activating cancer pathways. The effects of AB1 on the phenotype and epigenetics of both MCF7 and MCF10A cells associated with cancer development observed in this study suggest that AB1 is a potential risk factor for developing breast cancer. |
| first_indexed | 2025-11-15T13:42:24Z |
| format | Article |
| id | upm-103499 |
| institution | Universiti Putra Malaysia |
| institution_category | Local University |
| last_indexed | 2025-11-15T13:42:24Z |
| publishDate | 2022 |
| publisher | Multidisciplinary Digital Publishing Institute |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | upm-1034992023-05-24T04:43:07Z http://psasir.upm.edu.my/id/eprint/103499/ The effect of Aflatoxin B1 on tumor-related genes and phenotypic characters of MCF7 and MCF10A cells Ahmed Adam, Mowaffaq Adam Muhd Kamal, Laina Zarisa Kanakal, Mahibub Babu, Dinesh Dahham, Saad Sabbar Tabana, Yasser Lok, Bronwyn Bermoy, Brittany M. Yunus, Muhammad Amir Than, Leslie Thian Lung Barakat, Khaled Sandai, Doblin The fungal toxin aflatoxin B1 (AB1) and its reactive intermediate, aflatoxin B1-8, 9 epoxide, could cause liver cancer by inducing DNA adducts. AB1 exposure can induce changes in the expression of several cancer-related genes. In this study, the effect of AB1 exposure on breast cancer MCF7 and normal breast MCF10A cell lines at the phenotypic and epigenetic levels was investigated to evaluate its potential in increasing the risk of breast cancer development. We hypothesized that, even at low concentrations, AB1 can cause changes in the expression of important genes involved in four pathways, i.e., p53, cancer, cell cycle, and apoptosis. The transcriptomic levels of BRCA1, BRCA2, p53, HER1, HER2, cMyc, BCL2, MCL1, CCND1, WNT3A, MAPK1, MAPK3, DAPK1, Casp8, and Casp9 were determined in MCF7 and MCF10A cells. Our results illustrate that treating both cells with AB1 induced cytotoxicity and apoptosis with reduction in cell viability in a concentration-dependent manner. Additionally, AB1 reduced reactive oxygen species levels. Phenotypically, AB1 caused cell-cycle arrest at G1, hypertrophy, and increased cell migration rates. There were changes in the expression levels of several tumor-related genes, which are known to contribute to activating cancer pathways. The effects of AB1 on the phenotype and epigenetics of both MCF7 and MCF10A cells associated with cancer development observed in this study suggest that AB1 is a potential risk factor for developing breast cancer. Multidisciplinary Digital Publishing Institute 2022 Article PeerReviewed Ahmed Adam, Mowaffaq Adam and Muhd Kamal, Laina Zarisa and Kanakal, Mahibub and Babu, Dinesh and Dahham, Saad Sabbar and Tabana, Yasser and Lok, Bronwyn and Bermoy, Brittany M. and Yunus, Muhammad Amir and Than, Leslie Thian Lung and Barakat, Khaled and Sandai, Doblin (2022) The effect of Aflatoxin B1 on tumor-related genes and phenotypic characters of MCF7 and MCF10A cells. International Journal of Molecular Sciences, 23 (19). art. no. 11856. pp. 1-20. ISSN 1422-0067 https://www.mdpi.com/1422-0067/23/19/11856 10.3390/ijms231911856 |
| spellingShingle | Ahmed Adam, Mowaffaq Adam Muhd Kamal, Laina Zarisa Kanakal, Mahibub Babu, Dinesh Dahham, Saad Sabbar Tabana, Yasser Lok, Bronwyn Bermoy, Brittany M. Yunus, Muhammad Amir Than, Leslie Thian Lung Barakat, Khaled Sandai, Doblin The effect of Aflatoxin B1 on tumor-related genes and phenotypic characters of MCF7 and MCF10A cells |
| title | The effect of Aflatoxin B1 on tumor-related genes and phenotypic characters of MCF7 and MCF10A cells |
| title_full | The effect of Aflatoxin B1 on tumor-related genes and phenotypic characters of MCF7 and MCF10A cells |
| title_fullStr | The effect of Aflatoxin B1 on tumor-related genes and phenotypic characters of MCF7 and MCF10A cells |
| title_full_unstemmed | The effect of Aflatoxin B1 on tumor-related genes and phenotypic characters of MCF7 and MCF10A cells |
| title_short | The effect of Aflatoxin B1 on tumor-related genes and phenotypic characters of MCF7 and MCF10A cells |
| title_sort | effect of aflatoxin b1 on tumor-related genes and phenotypic characters of mcf7 and mcf10a cells |
| url | http://psasir.upm.edu.my/id/eprint/103499/ http://psasir.upm.edu.my/id/eprint/103499/ http://psasir.upm.edu.my/id/eprint/103499/ |