Synthesis of zinc oxide nanoparticles with bioflavonoid rutin: characterisation, antioxidant and antimicrobial activities and in vivo cytotoxic effects on Artemia Nauplii
This study aims to synthesise zinc oxide nanoparticles with rutin (ZnO-R NPs) using a green synthesis approach and characterise the nanostructures for diverse biomedical applications. In this study, the optical and chemical properties of synthesised ZnO-R NPs were verified through Fourier transform...
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| Format: | Article |
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Multidisciplinary Digital Publishing Institute
2022
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| Online Access: | http://psasir.upm.edu.my/id/eprint/103411/ |
| _version_ | 1848864013254918144 |
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| author | Saleemi, Mansab Ali Alallam, Batoul Yong, Yoke Keong Lim, Vuanghao |
| author_facet | Saleemi, Mansab Ali Alallam, Batoul Yong, Yoke Keong Lim, Vuanghao |
| author_sort | Saleemi, Mansab Ali |
| building | UPM Institutional Repository |
| collection | Online Access |
| description | This study aims to synthesise zinc oxide nanoparticles with rutin (ZnO-R NPs) using a green synthesis approach and characterise the nanostructures for diverse biomedical applications. In this study, the optical and chemical properties of synthesised ZnO-R NPs were verified through Fourier transform infrared (FTIR) spectroscopy and ultraviolet-visible (UV-Vis) spectroscopy. The FTIR spectroscopy revealed a symmetric bending vibration peak of 460 cm−1 for ZnO-R NPs, whereas UV-Vis spectroscopy showed a distinct absorption band at 395 nm. Moreover, the oval-shaped morphology of ZnO-R NPs was verified through scanning electron microscopy and transmission electron microscopy. The synthesised nanoformulation revealed a wurtzite structure with a crystallite size of 13.22 nm; however, the zeta potential value was recorded as −8.50 ± 0.46 mV for ZnO-R NPs. According to an antioxidant study, ZnO-R NPs demonstrated lower free-radical scavenging activity than pure rutin. The cytotoxicity study was conducted using a human breast cancer cell line (MCF-7). In vitro analysis verified that ZnO-R NPs exhibited significantly higher anticancer and microbial growth inhibition activities than standard ZnO NPs (ZnO Std NPs) and pure rutin. In addition, ZnO-R NPs revealed a significantly lower IC50 value than the commercial ZnO Std NPs and pure rutin in MCF-7 cells (16.39 ± 6.03 μg/mL, 27 ± 0.91 μg/mL and 350 ± 30.1 μg/mL, respectively) after 48 h. However, synthesised ZnO-R NPs demonstrated no significant toxicity towards Artemia nauplii. These results highlight the synthesis of rutin-mediated ZnO NPs and their possible chemotherapeutic potential. |
| first_indexed | 2025-11-15T13:42:03Z |
| format | Article |
| id | upm-103411 |
| institution | Universiti Putra Malaysia |
| institution_category | Local University |
| last_indexed | 2025-11-15T13:42:03Z |
| publishDate | 2022 |
| publisher | Multidisciplinary Digital Publishing Institute |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | upm-1034112023-06-09T03:20:17Z http://psasir.upm.edu.my/id/eprint/103411/ Synthesis of zinc oxide nanoparticles with bioflavonoid rutin: characterisation, antioxidant and antimicrobial activities and in vivo cytotoxic effects on Artemia Nauplii Saleemi, Mansab Ali Alallam, Batoul Yong, Yoke Keong Lim, Vuanghao This study aims to synthesise zinc oxide nanoparticles with rutin (ZnO-R NPs) using a green synthesis approach and characterise the nanostructures for diverse biomedical applications. In this study, the optical and chemical properties of synthesised ZnO-R NPs were verified through Fourier transform infrared (FTIR) spectroscopy and ultraviolet-visible (UV-Vis) spectroscopy. The FTIR spectroscopy revealed a symmetric bending vibration peak of 460 cm−1 for ZnO-R NPs, whereas UV-Vis spectroscopy showed a distinct absorption band at 395 nm. Moreover, the oval-shaped morphology of ZnO-R NPs was verified through scanning electron microscopy and transmission electron microscopy. The synthesised nanoformulation revealed a wurtzite structure with a crystallite size of 13.22 nm; however, the zeta potential value was recorded as −8.50 ± 0.46 mV for ZnO-R NPs. According to an antioxidant study, ZnO-R NPs demonstrated lower free-radical scavenging activity than pure rutin. The cytotoxicity study was conducted using a human breast cancer cell line (MCF-7). In vitro analysis verified that ZnO-R NPs exhibited significantly higher anticancer and microbial growth inhibition activities than standard ZnO NPs (ZnO Std NPs) and pure rutin. In addition, ZnO-R NPs revealed a significantly lower IC50 value than the commercial ZnO Std NPs and pure rutin in MCF-7 cells (16.39 ± 6.03 μg/mL, 27 ± 0.91 μg/mL and 350 ± 30.1 μg/mL, respectively) after 48 h. However, synthesised ZnO-R NPs demonstrated no significant toxicity towards Artemia nauplii. These results highlight the synthesis of rutin-mediated ZnO NPs and their possible chemotherapeutic potential. Multidisciplinary Digital Publishing Institute 2022 Article PeerReviewed Saleemi, Mansab Ali and Alallam, Batoul and Yong, Yoke Keong and Lim, Vuanghao (2022) Synthesis of zinc oxide nanoparticles with bioflavonoid rutin: characterisation, antioxidant and antimicrobial activities and in vivo cytotoxic effects on Artemia Nauplii. Antioxidants, 11 (10). art. no. 1853. pp. 1-19. ISSN 2076-3921 https://www.mdpi.com/2076-3921/11/10/1853 10.3390/antiox11101853 |
| spellingShingle | Saleemi, Mansab Ali Alallam, Batoul Yong, Yoke Keong Lim, Vuanghao Synthesis of zinc oxide nanoparticles with bioflavonoid rutin: characterisation, antioxidant and antimicrobial activities and in vivo cytotoxic effects on Artemia Nauplii |
| title | Synthesis of zinc oxide nanoparticles with bioflavonoid rutin: characterisation, antioxidant and antimicrobial activities and in vivo cytotoxic effects on Artemia Nauplii |
| title_full | Synthesis of zinc oxide nanoparticles with bioflavonoid rutin: characterisation, antioxidant and antimicrobial activities and in vivo cytotoxic effects on Artemia Nauplii |
| title_fullStr | Synthesis of zinc oxide nanoparticles with bioflavonoid rutin: characterisation, antioxidant and antimicrobial activities and in vivo cytotoxic effects on Artemia Nauplii |
| title_full_unstemmed | Synthesis of zinc oxide nanoparticles with bioflavonoid rutin: characterisation, antioxidant and antimicrobial activities and in vivo cytotoxic effects on Artemia Nauplii |
| title_short | Synthesis of zinc oxide nanoparticles with bioflavonoid rutin: characterisation, antioxidant and antimicrobial activities and in vivo cytotoxic effects on Artemia Nauplii |
| title_sort | synthesis of zinc oxide nanoparticles with bioflavonoid rutin: characterisation, antioxidant and antimicrobial activities and in vivo cytotoxic effects on artemia nauplii |
| url | http://psasir.upm.edu.my/id/eprint/103411/ http://psasir.upm.edu.my/id/eprint/103411/ http://psasir.upm.edu.my/id/eprint/103411/ |