Solid-phase extraction and characterization of Quercetrin-rich fraction from Melastoma malabathricum leaves
This study was focused on the recovery of quercetrin from the crude extract of Melastoma malabathricum leaves using the technique of solid-phase extraction. The process variables and their ranges were screened using one-factor-at-a-time and statistically optimized using the response surface methodol...
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| Format: | Article |
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Multidisciplinary Digital Publishing Institute
2022
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| Online Access: | http://psasir.upm.edu.my/id/eprint/103250/ |
| _version_ | 1848863970721529856 |
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| author | Awang, Mohd Azrie Lee, Suan Chua Abdullah, Luqman Chuah |
| author_facet | Awang, Mohd Azrie Lee, Suan Chua Abdullah, Luqman Chuah |
| author_sort | Awang, Mohd Azrie |
| building | UPM Institutional Repository |
| collection | Online Access |
| description | This study was focused on the recovery of quercetrin from the crude extract of Melastoma malabathricum leaves using the technique of solid-phase extraction. The process variables and their ranges were screened using one-factor-at-a-time and statistically optimized using the response surface methodology. The results found that 9.13 mg/mL of crude extract required 18.24 mL of 70.5% methanol as the eluent to yield an 86.6% w/w fraction containing 36.02 mg/g of quercetrin. The process increased quercetrin from 1% w/w in the crude extract to 4% w/w in the fraction. Quercetrin was likely to be the compound contributing to antiradical and antidiabetic activities. In silico simulation showed that quercetrin had low binding energy and mostly bound with charged (Glu and Arg) and aromatic (Tyr and Phe) amino acids via hydrogen bonds. Its inhibitory progress against DPP-IV was faster than crude extract at low concentration (<100 µg/mL). |
| first_indexed | 2025-11-15T13:41:23Z |
| format | Article |
| id | upm-103250 |
| institution | Universiti Putra Malaysia |
| institution_category | Local University |
| last_indexed | 2025-11-15T13:41:23Z |
| publishDate | 2022 |
| publisher | Multidisciplinary Digital Publishing Institute |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | upm-1032502023-11-16T04:43:37Z http://psasir.upm.edu.my/id/eprint/103250/ Solid-phase extraction and characterization of Quercetrin-rich fraction from Melastoma malabathricum leaves Awang, Mohd Azrie Lee, Suan Chua Abdullah, Luqman Chuah This study was focused on the recovery of quercetrin from the crude extract of Melastoma malabathricum leaves using the technique of solid-phase extraction. The process variables and their ranges were screened using one-factor-at-a-time and statistically optimized using the response surface methodology. The results found that 9.13 mg/mL of crude extract required 18.24 mL of 70.5% methanol as the eluent to yield an 86.6% w/w fraction containing 36.02 mg/g of quercetrin. The process increased quercetrin from 1% w/w in the crude extract to 4% w/w in the fraction. Quercetrin was likely to be the compound contributing to antiradical and antidiabetic activities. In silico simulation showed that quercetrin had low binding energy and mostly bound with charged (Glu and Arg) and aromatic (Tyr and Phe) amino acids via hydrogen bonds. Its inhibitory progress against DPP-IV was faster than crude extract at low concentration (<100 µg/mL). Multidisciplinary Digital Publishing Institute 2022 Article PeerReviewed Awang, Mohd Azrie and Lee, Suan Chua and Abdullah, Luqman Chuah (2022) Solid-phase extraction and characterization of Quercetrin-rich fraction from Melastoma malabathricum leaves. Separations, 9 (11). art. no. 373. pp. 1-17. ISSN 2297-8739 https://www.mdpi.com/2297-8739/9/11/373 10.3390/separations9110373 |
| spellingShingle | Awang, Mohd Azrie Lee, Suan Chua Abdullah, Luqman Chuah Solid-phase extraction and characterization of Quercetrin-rich fraction from Melastoma malabathricum leaves |
| title | Solid-phase extraction and characterization of Quercetrin-rich fraction from Melastoma malabathricum leaves |
| title_full | Solid-phase extraction and characterization of Quercetrin-rich fraction from Melastoma malabathricum leaves |
| title_fullStr | Solid-phase extraction and characterization of Quercetrin-rich fraction from Melastoma malabathricum leaves |
| title_full_unstemmed | Solid-phase extraction and characterization of Quercetrin-rich fraction from Melastoma malabathricum leaves |
| title_short | Solid-phase extraction and characterization of Quercetrin-rich fraction from Melastoma malabathricum leaves |
| title_sort | solid-phase extraction and characterization of quercetrin-rich fraction from melastoma malabathricum leaves |
| url | http://psasir.upm.edu.my/id/eprint/103250/ http://psasir.upm.edu.my/id/eprint/103250/ http://psasir.upm.edu.my/id/eprint/103250/ |