Proteomic analysis revealed the biofilm-degradation abilities of the bacteriophage UPMK_1 and UPMK_2 against Methicillin-resistant Staphylococcus aureus
Objective: The degradation activity of two bacteriophages UPMK_1 and UPMK_2 against methicillin-resistant Staphylococcus aureus phages were examined using gel zymography. Methods: The analysis was done using BLASTP to detect peptides catalytic domains. Many peptides that are related to several phage...
| Main Authors: | , , , , , , , |
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| Format: | Article |
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Springer
2022
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| Online Access: | http://psasir.upm.edu.my/id/eprint/102935/ |
| _version_ | 1848863902293557248 |
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| author | Dakheel, Khulood Hamid Abdul Rahim, Raha Al-Obaidi, Jameel R Kumari Neela, Vasantha Tan, Geok Hun Mat Isa, Mohd Noor Razali, Nurhanani Yusoff, Khatijah |
| author_facet | Dakheel, Khulood Hamid Abdul Rahim, Raha Al-Obaidi, Jameel R Kumari Neela, Vasantha Tan, Geok Hun Mat Isa, Mohd Noor Razali, Nurhanani Yusoff, Khatijah |
| author_sort | Dakheel, Khulood Hamid |
| building | UPM Institutional Repository |
| collection | Online Access |
| description | Objective: The degradation activity of two bacteriophages UPMK_1 and UPMK_2 against methicillin-resistant Staphylococcus aureus phages were examined using gel zymography. Methods: The analysis was done using BLASTP to detect peptides catalytic domains. Many peptides that are related to several phage proteins were revealed. Results: UPMK_1 and UPMK_2 custom sequence database were used for peptide identification. The biofilm-degrading proteins in the bacteriophage UPMK_2 revealed the same lytic activity towards polysaccharide intercellular adhesin-dependent and independent of Methicillin-resistant Staphylococcus aureus (MRSA) biofilm producers in comparison to UPMK_1, which had lytic activity restricted solely to its host. Conclusion: Both bacteriophage enzymes were involved in MRSA biofilm degradation during phage infection and they have promising enzybiotics properties against MRSA biofilm formation. |
| first_indexed | 2025-11-15T13:40:18Z |
| format | Article |
| id | upm-102935 |
| institution | Universiti Putra Malaysia |
| institution_category | Local University |
| last_indexed | 2025-11-15T13:40:18Z |
| publishDate | 2022 |
| publisher | Springer |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | upm-1029352024-11-21T04:51:43Z http://psasir.upm.edu.my/id/eprint/102935/ Proteomic analysis revealed the biofilm-degradation abilities of the bacteriophage UPMK_1 and UPMK_2 against Methicillin-resistant Staphylococcus aureus Dakheel, Khulood Hamid Abdul Rahim, Raha Al-Obaidi, Jameel R Kumari Neela, Vasantha Tan, Geok Hun Mat Isa, Mohd Noor Razali, Nurhanani Yusoff, Khatijah Objective: The degradation activity of two bacteriophages UPMK_1 and UPMK_2 against methicillin-resistant Staphylococcus aureus phages were examined using gel zymography. Methods: The analysis was done using BLASTP to detect peptides catalytic domains. Many peptides that are related to several phage proteins were revealed. Results: UPMK_1 and UPMK_2 custom sequence database were used for peptide identification. The biofilm-degrading proteins in the bacteriophage UPMK_2 revealed the same lytic activity towards polysaccharide intercellular adhesin-dependent and independent of Methicillin-resistant Staphylococcus aureus (MRSA) biofilm producers in comparison to UPMK_1, which had lytic activity restricted solely to its host. Conclusion: Both bacteriophage enzymes were involved in MRSA biofilm degradation during phage infection and they have promising enzybiotics properties against MRSA biofilm formation. Springer 2022 Article PeerReviewed Dakheel, Khulood Hamid and Abdul Rahim, Raha and Al-Obaidi, Jameel R and Kumari Neela, Vasantha and Tan, Geok Hun and Mat Isa, Mohd Noor and Razali, Nurhanani and Yusoff, Khatijah (2022) Proteomic analysis revealed the biofilm-degradation abilities of the bacteriophage UPMK_1 and UPMK_2 against Methicillin-resistant Staphylococcus aureus. Biotechnology Letters, 44 (3). pp. 513-522. ISSN 0141-5492; eISSN: 1573-6776 https://link.springer.com/article/10.1007/s10529-022-03229-y 10.1007/s10529-022-03229-y |
| spellingShingle | Dakheel, Khulood Hamid Abdul Rahim, Raha Al-Obaidi, Jameel R Kumari Neela, Vasantha Tan, Geok Hun Mat Isa, Mohd Noor Razali, Nurhanani Yusoff, Khatijah Proteomic analysis revealed the biofilm-degradation abilities of the bacteriophage UPMK_1 and UPMK_2 against Methicillin-resistant Staphylococcus aureus |
| title | Proteomic analysis revealed the biofilm-degradation abilities of the bacteriophage UPMK_1 and UPMK_2 against Methicillin-resistant Staphylococcus aureus |
| title_full | Proteomic analysis revealed the biofilm-degradation abilities of the bacteriophage UPMK_1 and UPMK_2 against Methicillin-resistant Staphylococcus aureus |
| title_fullStr | Proteomic analysis revealed the biofilm-degradation abilities of the bacteriophage UPMK_1 and UPMK_2 against Methicillin-resistant Staphylococcus aureus |
| title_full_unstemmed | Proteomic analysis revealed the biofilm-degradation abilities of the bacteriophage UPMK_1 and UPMK_2 against Methicillin-resistant Staphylococcus aureus |
| title_short | Proteomic analysis revealed the biofilm-degradation abilities of the bacteriophage UPMK_1 and UPMK_2 against Methicillin-resistant Staphylococcus aureus |
| title_sort | proteomic analysis revealed the biofilm-degradation abilities of the bacteriophage upmk_1 and upmk_2 against methicillin-resistant staphylococcus aureus |
| url | http://psasir.upm.edu.my/id/eprint/102935/ http://psasir.upm.edu.my/id/eprint/102935/ http://psasir.upm.edu.my/id/eprint/102935/ |