Preparation, characterization and in vitro cytotoxicity evaluation of free and liposome-encapsulated tylosin
Liposomes are the most attractive and well-known nanoparticles due to their high biocompatibility and biodegradability features which make them display less toxic effects for employing in vivo. Encapsulation of antibiotics within liposomes can provide a promising strategy for the effective treatment...
| Main Authors: | , , , , |
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| Format: | Article |
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Elsevier BV
2022
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| Online Access: | http://psasir.upm.edu.my/id/eprint/102859/ |
| _version_ | 1848863891104202752 |
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| author | Saddiqi, Mohammad Ehsan Abdul Kadir, Arifah Abdullah, Faez Firdaus Jesse Abu Bakar Zakaria, Md Zuki Banke, Idris Sherifat |
| author_facet | Saddiqi, Mohammad Ehsan Abdul Kadir, Arifah Abdullah, Faez Firdaus Jesse Abu Bakar Zakaria, Md Zuki Banke, Idris Sherifat |
| author_sort | Saddiqi, Mohammad Ehsan |
| building | UPM Institutional Repository |
| collection | Online Access |
| description | Liposomes are the most attractive and well-known nanoparticles due to their high biocompatibility and biodegradability features which make them display less toxic effects for employing in vivo. Encapsulation of antibiotics within liposomes can provide a promising strategy for the effective treatment of resistant bacterial pathogens. This research was performed to prepare, characterize and evaluate the in vitro cytotoxicity effect of free and liposomal tylosin. Liposomal tylosin was produced by the thin-film hydration method. The characterization was performed by dynamic light scattering and high resolution transmission electron microscopy. Methylthiazol tetrazolium (MTT) assay was applied to evaluate the cytotoxicity of free and liposome-encapsulated tylosin against the NIH3T3 and NHDF. The obtained findings indicated well-formed liposome with ideal characteristics that efficiently encapsulated tylosin. The cytotoxicity data revealed cell viability of above 70% for liposomal tylosin and below 70% for free tylosin towards both NIH3T3 and NHDF at concentration of 1024 µg/mL. Therefore, liposome is a promising vehicle for an antibiotic delivery system with appropriate physicochemical, drug loading, release characteristics, and ideal biocompatibility properties. |
| first_indexed | 2025-11-15T13:40:07Z |
| format | Article |
| id | upm-102859 |
| institution | Universiti Putra Malaysia |
| institution_category | Local University |
| last_indexed | 2025-11-15T13:40:07Z |
| publishDate | 2022 |
| publisher | Elsevier BV |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | upm-1028592024-06-30T15:01:19Z http://psasir.upm.edu.my/id/eprint/102859/ Preparation, characterization and in vitro cytotoxicity evaluation of free and liposome-encapsulated tylosin Saddiqi, Mohammad Ehsan Abdul Kadir, Arifah Abdullah, Faez Firdaus Jesse Abu Bakar Zakaria, Md Zuki Banke, Idris Sherifat Liposomes are the most attractive and well-known nanoparticles due to their high biocompatibility and biodegradability features which make them display less toxic effects for employing in vivo. Encapsulation of antibiotics within liposomes can provide a promising strategy for the effective treatment of resistant bacterial pathogens. This research was performed to prepare, characterize and evaluate the in vitro cytotoxicity effect of free and liposomal tylosin. Liposomal tylosin was produced by the thin-film hydration method. The characterization was performed by dynamic light scattering and high resolution transmission electron microscopy. Methylthiazol tetrazolium (MTT) assay was applied to evaluate the cytotoxicity of free and liposome-encapsulated tylosin against the NIH3T3 and NHDF. The obtained findings indicated well-formed liposome with ideal characteristics that efficiently encapsulated tylosin. The cytotoxicity data revealed cell viability of above 70% for liposomal tylosin and below 70% for free tylosin towards both NIH3T3 and NHDF at concentration of 1024 µg/mL. Therefore, liposome is a promising vehicle for an antibiotic delivery system with appropriate physicochemical, drug loading, release characteristics, and ideal biocompatibility properties. Elsevier BV 2022 Article PeerReviewed Saddiqi, Mohammad Ehsan and Abdul Kadir, Arifah and Abdullah, Faez Firdaus Jesse and Abu Bakar Zakaria, Md Zuki and Banke, Idris Sherifat (2022) Preparation, characterization and in vitro cytotoxicity evaluation of free and liposome-encapsulated tylosin. Opennano, 8. art. no. 100108. pp. 1-10. ISSN 2352-9520 https://linkinghub.elsevier.com/retrieve/pii/S2352952022000706 10.1016/j.onano.2022.100108 |
| spellingShingle | Saddiqi, Mohammad Ehsan Abdul Kadir, Arifah Abdullah, Faez Firdaus Jesse Abu Bakar Zakaria, Md Zuki Banke, Idris Sherifat Preparation, characterization and in vitro cytotoxicity evaluation of free and liposome-encapsulated tylosin |
| title | Preparation, characterization and in vitro cytotoxicity evaluation of free and liposome-encapsulated tylosin |
| title_full | Preparation, characterization and in vitro cytotoxicity evaluation of free and liposome-encapsulated tylosin |
| title_fullStr | Preparation, characterization and in vitro cytotoxicity evaluation of free and liposome-encapsulated tylosin |
| title_full_unstemmed | Preparation, characterization and in vitro cytotoxicity evaluation of free and liposome-encapsulated tylosin |
| title_short | Preparation, characterization and in vitro cytotoxicity evaluation of free and liposome-encapsulated tylosin |
| title_sort | preparation, characterization and in vitro cytotoxicity evaluation of free and liposome-encapsulated tylosin |
| url | http://psasir.upm.edu.my/id/eprint/102859/ http://psasir.upm.edu.my/id/eprint/102859/ http://psasir.upm.edu.my/id/eprint/102859/ |