Polarity affects the antioxidant and antimicrobial activities of jellyfish Acromitus hardenbergi extracts
Jellyfish is an emerging aquaculture species, farmed for Oriental cuisines and nutraceutical ingredients. This study aimed to examine antioxidative and antimicrobial potentials of various fractions of the jellyfish, Acromitus hardenbergi. The bell and oral arms of the jellyfish were sequentially ext...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
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Korean Society of Fisheries and Aquatic Sciences
2022
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| Online Access: | http://psasir.upm.edu.my/id/eprint/102793/ http://psasir.upm.edu.my/id/eprint/102793/1/102793.pdf |
| _version_ | 1848863874042822656 |
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| author | Khong, Nicholas M. H. Foo, Su Chern Yau, Sook Kun Chan, Kim Wei Md. Yusoff, Fatimah |
| author_facet | Khong, Nicholas M. H. Foo, Su Chern Yau, Sook Kun Chan, Kim Wei Md. Yusoff, Fatimah |
| author_sort | Khong, Nicholas M. H. |
| building | UPM Institutional Repository |
| collection | Online Access |
| description | Jellyfish is an emerging aquaculture species, farmed for Oriental cuisines and nutraceutical ingredients. This study aimed to examine antioxidative and antimicrobial potentials of various fractions of the jellyfish, Acromitus hardenbergi. The bell and oral arms of the jellyfish were sequentially extracted with petroleum ether (PE), dichloromethane (DCM), chloroform (CHCl3), methanol (MeOH), and water (H2O) to extract its bioactive in an increasing polarity gradient. Test fractions were assayed for antiradical activities using electron spin resonance spectrometry, β-carotene-linoleate model and Folin-Ciocalteu assay; and antimicrobial activity against 2 Gram-negative bacteria, 4 Gram-positive bacteria and 2 fungal species using the disc diffusion assay. All fractions were also subjected to Fourier Transform Infrared (FTIR) analysis to identify types of functional groups present. It was found that the hydrophilic extracts (H2O fractions) possessed the most effective radical scavenging activity (p < 0.05) while the lipophilic extracts (PE fractions) the most active antimicrobial activity, especially against Gram-positive bacteria (p < 0.05). Total oxidation substrates content was found to be highest in the PE fractions of jellyfish bell and oral arms (p < 0.05). FTIR data showed that the H2O and MeOH fractions contains similar functional groups including -OH, -C=O, -N-H and -S=O groups, while the PE, DCM, and CHCl3 fractions, the -CH3, -COOH groups. This study showed that A. hardenbergi contains antioxidants and antimicrobials, thereby supporting the traditional claim of the jellyfish as an anti-aging and health-promoting functional food. Bioassay-guided fractionation approach serves as a critical milestone for the strategic screening, purification, and elucidation of therapeutically significant actives from jellyfish. |
| first_indexed | 2025-11-15T13:39:51Z |
| format | Article |
| id | upm-102793 |
| institution | Universiti Putra Malaysia |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-15T13:39:51Z |
| publishDate | 2022 |
| publisher | Korean Society of Fisheries and Aquatic Sciences |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | upm-1027932025-07-09T07:35:58Z http://psasir.upm.edu.my/id/eprint/102793/ Polarity affects the antioxidant and antimicrobial activities of jellyfish Acromitus hardenbergi extracts Khong, Nicholas M. H. Foo, Su Chern Yau, Sook Kun Chan, Kim Wei Md. Yusoff, Fatimah Jellyfish is an emerging aquaculture species, farmed for Oriental cuisines and nutraceutical ingredients. This study aimed to examine antioxidative and antimicrobial potentials of various fractions of the jellyfish, Acromitus hardenbergi. The bell and oral arms of the jellyfish were sequentially extracted with petroleum ether (PE), dichloromethane (DCM), chloroform (CHCl3), methanol (MeOH), and water (H2O) to extract its bioactive in an increasing polarity gradient. Test fractions were assayed for antiradical activities using electron spin resonance spectrometry, β-carotene-linoleate model and Folin-Ciocalteu assay; and antimicrobial activity against 2 Gram-negative bacteria, 4 Gram-positive bacteria and 2 fungal species using the disc diffusion assay. All fractions were also subjected to Fourier Transform Infrared (FTIR) analysis to identify types of functional groups present. It was found that the hydrophilic extracts (H2O fractions) possessed the most effective radical scavenging activity (p < 0.05) while the lipophilic extracts (PE fractions) the most active antimicrobial activity, especially against Gram-positive bacteria (p < 0.05). Total oxidation substrates content was found to be highest in the PE fractions of jellyfish bell and oral arms (p < 0.05). FTIR data showed that the H2O and MeOH fractions contains similar functional groups including -OH, -C=O, -N-H and -S=O groups, while the PE, DCM, and CHCl3 fractions, the -CH3, -COOH groups. This study showed that A. hardenbergi contains antioxidants and antimicrobials, thereby supporting the traditional claim of the jellyfish as an anti-aging and health-promoting functional food. Bioassay-guided fractionation approach serves as a critical milestone for the strategic screening, purification, and elucidation of therapeutically significant actives from jellyfish. Korean Society of Fisheries and Aquatic Sciences 2022 Article PeerReviewed text en cc_by_nc_4 http://psasir.upm.edu.my/id/eprint/102793/1/102793.pdf Khong, Nicholas M. H. and Foo, Su Chern and Yau, Sook Kun and Chan, Kim Wei and Md. Yusoff, Fatimah (2022) Polarity affects the antioxidant and antimicrobial activities of jellyfish Acromitus hardenbergi extracts. Fisheries and Aquatic Sciences, 25 (4). pp. 189-201. ISSN 2234-1749; eISSN: 2234-1757 https://www.e-fas.org/archive/view_article?pid=fas-25-4-189 10.47853/FAS.2022.e18 |
| spellingShingle | Khong, Nicholas M. H. Foo, Su Chern Yau, Sook Kun Chan, Kim Wei Md. Yusoff, Fatimah Polarity affects the antioxidant and antimicrobial activities of jellyfish Acromitus hardenbergi extracts |
| title | Polarity affects the antioxidant and antimicrobial activities of jellyfish Acromitus hardenbergi extracts |
| title_full | Polarity affects the antioxidant and antimicrobial activities of jellyfish Acromitus hardenbergi extracts |
| title_fullStr | Polarity affects the antioxidant and antimicrobial activities of jellyfish Acromitus hardenbergi extracts |
| title_full_unstemmed | Polarity affects the antioxidant and antimicrobial activities of jellyfish Acromitus hardenbergi extracts |
| title_short | Polarity affects the antioxidant and antimicrobial activities of jellyfish Acromitus hardenbergi extracts |
| title_sort | polarity affects the antioxidant and antimicrobial activities of jellyfish acromitus hardenbergi extracts |
| url | http://psasir.upm.edu.my/id/eprint/102793/ http://psasir.upm.edu.my/id/eprint/102793/ http://psasir.upm.edu.my/id/eprint/102793/ http://psasir.upm.edu.my/id/eprint/102793/1/102793.pdf |