Pineapple peel as alternative substrate for bacterial nanocellulose production
Due to its flexible properties, bacterial nanocellulose (BNC) has been attracting tremendous attention. In this study, BNC was produced by Acetobacter xylinum ATCC2376 and a local isolate, namely, Bacillus cereus MMS1. The production of BNC was done by utilising pineapple peel extract (PPE) (wastes...
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| Format: | Article |
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Springer
2022
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| Online Access: | http://psasir.upm.edu.my/id/eprint/102784/ |
| _version_ | 1848863871836618752 |
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| author | Lee, Adriana Connie Md Salleh, Madihah Ibrahim, Mohamad Faizal Bahrin, Ezyana Kamal Jenol, Mohd Azwan Abd-Aziz, Suraini |
| author_facet | Lee, Adriana Connie Md Salleh, Madihah Ibrahim, Mohamad Faizal Bahrin, Ezyana Kamal Jenol, Mohd Azwan Abd-Aziz, Suraini |
| author_sort | Lee, Adriana Connie |
| building | UPM Institutional Repository |
| collection | Online Access |
| description | Due to its flexible properties, bacterial nanocellulose (BNC) has been attracting tremendous attention. In this study, BNC was produced by Acetobacter xylinum ATCC2376 and a local isolate, namely, Bacillus cereus MMS1. The production of BNC was done by utilising pineapple peel extract (PPE) (wastes discarded after cutting the fruit) as the alternative carbon source substituting the commercial D-glucose (control) in Hestrin–Schramm (HS) medium under agitated conditions. This research is aimed to investigate the synthesis of BNC by an isolated bacterial strain from termite’s gut using an agro-industrial waste which is the pineapple peel extract. Six bacterial strains, namely, F8, F5, M1, M6, H7 and H11, were screened and identified for potential BNC producer. The selected bacterial strain was identified as Bacillus cereus MMS1 using 16S rRNA nucleotide sequences. Then, the production of BNC was done by B. cereus MMS1 using pineapple peel extract, while A. xylinum ATCC2376 acted as a control. The BNC production in this study was attained at 2% (w/v) glucose concentration, 12 days of incubation period and 150 rpm agitation speed which was 5.83 g/L by A. xylinum ATCC2376 in HS medium using commercial glucose as carbon source. Meanwhile, 4.42 g/L and 2 g/L of BNC were produced by B. cereus MMS1 after 12 days of incubation with an initial concentration of 2% (w/v) using commercial glucose and pineapple peel extract, respectively. |
| first_indexed | 2025-11-15T13:39:49Z |
| format | Article |
| id | upm-102784 |
| institution | Universiti Putra Malaysia |
| institution_category | Local University |
| last_indexed | 2025-11-15T13:39:49Z |
| publishDate | 2022 |
| publisher | Springer |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | upm-1027842024-06-30T01:39:07Z http://psasir.upm.edu.my/id/eprint/102784/ Pineapple peel as alternative substrate for bacterial nanocellulose production Lee, Adriana Connie Md Salleh, Madihah Ibrahim, Mohamad Faizal Bahrin, Ezyana Kamal Jenol, Mohd Azwan Abd-Aziz, Suraini Due to its flexible properties, bacterial nanocellulose (BNC) has been attracting tremendous attention. In this study, BNC was produced by Acetobacter xylinum ATCC2376 and a local isolate, namely, Bacillus cereus MMS1. The production of BNC was done by utilising pineapple peel extract (PPE) (wastes discarded after cutting the fruit) as the alternative carbon source substituting the commercial D-glucose (control) in Hestrin–Schramm (HS) medium under agitated conditions. This research is aimed to investigate the synthesis of BNC by an isolated bacterial strain from termite’s gut using an agro-industrial waste which is the pineapple peel extract. Six bacterial strains, namely, F8, F5, M1, M6, H7 and H11, were screened and identified for potential BNC producer. The selected bacterial strain was identified as Bacillus cereus MMS1 using 16S rRNA nucleotide sequences. Then, the production of BNC was done by B. cereus MMS1 using pineapple peel extract, while A. xylinum ATCC2376 acted as a control. The BNC production in this study was attained at 2% (w/v) glucose concentration, 12 days of incubation period and 150 rpm agitation speed which was 5.83 g/L by A. xylinum ATCC2376 in HS medium using commercial glucose as carbon source. Meanwhile, 4.42 g/L and 2 g/L of BNC were produced by B. cereus MMS1 after 12 days of incubation with an initial concentration of 2% (w/v) using commercial glucose and pineapple peel extract, respectively. Springer 2022 Article PeerReviewed Lee, Adriana Connie and Md Salleh, Madihah and Ibrahim, Mohamad Faizal and Bahrin, Ezyana Kamal and Jenol, Mohd Azwan and Abd-Aziz, Suraini (2022) Pineapple peel as alternative substrate for bacterial nanocellulose production. Biomass Conversion and Biorefinery, 14. pp. 5541-5549. ISSN 2190-6815; ESSN: 2190-6823 https://link.springer.com/article/10.1007/s13399-022-03169-7 10.1007/s13399-022-03169-7 |
| spellingShingle | Lee, Adriana Connie Md Salleh, Madihah Ibrahim, Mohamad Faizal Bahrin, Ezyana Kamal Jenol, Mohd Azwan Abd-Aziz, Suraini Pineapple peel as alternative substrate for bacterial nanocellulose production |
| title | Pineapple peel as alternative substrate for bacterial nanocellulose production |
| title_full | Pineapple peel as alternative substrate for bacterial nanocellulose production |
| title_fullStr | Pineapple peel as alternative substrate for bacterial nanocellulose production |
| title_full_unstemmed | Pineapple peel as alternative substrate for bacterial nanocellulose production |
| title_short | Pineapple peel as alternative substrate for bacterial nanocellulose production |
| title_sort | pineapple peel as alternative substrate for bacterial nanocellulose production |
| url | http://psasir.upm.edu.my/id/eprint/102784/ http://psasir.upm.edu.my/id/eprint/102784/ http://psasir.upm.edu.my/id/eprint/102784/ |