Optimization of signal peptide via site-directed mutagenesis for enhanced secretion of heterologous proteins in lactococcus lactis
Secretion efficiency of heterologous proteins in the Generally Regarded As Safe (GRAS) Lactococcus lactis is often reported to be insufficiently low due to limitations such as poor targeting and translocation by the signal peptide or degradation by the host proteases. In this study, the secretion ef...
| Main Authors: | Alias, Nur Aqlili Riana, Song, Adelene Ai Lian, Alitheen, Noorjahan Banu, Rahim, Raha Abdul, Othman, Siti Sarah, In, Lionel Lian Aun |
|---|---|
| Format: | Article |
| Published: |
MDPI AG
2022
|
| Online Access: | http://psasir.upm.edu.my/id/eprint/102407/ |
Similar Items
Effect of secretion efficiency of mutant KRAS neoantigen by Lactococcus lactis on the immune response of a mucosal vaccine delivery vehicle targeting colorectal cancer
by: Alias, Nur Aqlili Riana, et al.
Published: (2023)
by: Alias, Nur Aqlili Riana, et al.
Published: (2023)
Secretion of recombinant xylanase in Lactococcus lactis using signal peptides Usp45 and Spk1
by: Roslan, Abdullah Munir, et al.
Published: (2020)
by: Roslan, Abdullah Munir, et al.
Published: (2020)
Engineering Lactococcus lactis as a cell factory for the production of limonene
by: Nurul ‘Aishah Shaili,, et al.
Published: (2022)
by: Nurul ‘Aishah Shaili,, et al.
Published: (2022)
Engineering Lactococcus lactis as a cell factory for the production of limonene
by: Shaili, Nurul ‘Aishah, et al.
Published: (2022)
by: Shaili, Nurul ‘Aishah, et al.
Published: (2022)
Cloning and in silico characterization of two signal peptides from Pediococcus pentosaceus and their function for the secretion of heterologous protein in Lactococcus lactis.
by: Baradaran, Ali, et al.
Published: (2013)
by: Baradaran, Ali, et al.
Published: (2013)
A review on Lactococcus lactis: from food to factory
by: Song, Adelene Ai‑Lian, et al.
Published: (2017)
by: Song, Adelene Ai‑Lian, et al.
Published: (2017)
Modification of signal peptide for enhanced secretion in Lactoccoccus lactis for oral vaccine delivery
by: Alias, Nur Aqlili Riana
Published: (2021)
by: Alias, Nur Aqlili Riana
Published: (2021)
Functional expression of plant sesquitepene synthases for isoprenoid production in lactococcus lactis
by: Song, Adelene Ai Lian
Published: (2012)
by: Song, Adelene Ai Lian
Published: (2012)
Heterologous expression and secretion of Bacillus coagulans ST-6 xylanase in Lactococcus lactis NZ9000
by: Roslan, Abdullah Munir
Published: (2017)
by: Roslan, Abdullah Munir
Published: (2017)
Lactococcus lactis secreting phage lysins as a potential antimicrobial against multi-drug resistant Staphylococcus aureus
by: Chandran, Carumathy, et al.
Published: (2022)
by: Chandran, Carumathy, et al.
Published: (2022)
Development of Secretory Lactococcus Lactis Vectors with Characterized Heterologous Signal Peptide from Pediococcus Pentosaceus
by: Baradaran, Ali
Published: (2010)
by: Baradaran, Ali
Published: (2010)
Lactococcus lactis harbouring Ara h 2.02 alleviates allergen-specific Th2-associated responses in sensitized mice
by: Chan, Chong Joo, et al.
Published: (2020)
by: Chan, Chong Joo, et al.
Published: (2020)
Lactococcus lactis M4, a potential host for the expression of heterologous proteins
by: Nanyan, Noreen, et al.
Published: (2011)
by: Nanyan, Noreen, et al.
Published: (2011)
Engineering integrative vectors based on bacteriophage site specific recombination mechanism for heterologous expression of secreted and surface-anchored protein in Lactococcus lactis
by: Koko, Innanurdiani
Published: (2019)
by: Koko, Innanurdiani
Published: (2019)
Effects of signal peptides on secretion of the bacillus g1 β-cyclodextrin glucanotransferase in lactococcus lactis NZ9000
by: Subramaniam, Menaga
Published: (2013)
by: Subramaniam, Menaga
Published: (2013)
Effects of signal peptides on secretion of the Bacillus G1 β-cyclodextrin glucanotransferase in Lactococcus lactis NZ9000
by: Subramaniam, Menaga
Published: (2013)
by: Subramaniam, Menaga
Published: (2013)
Surface display of glycosylated Tyrosinase related protein-2 (TRP-2) tumour antigen on Lactococcus lactis
by: Kalyanasundram, Jeevanathan, et al.
Published: (2015)
by: Kalyanasundram, Jeevanathan, et al.
Published: (2015)
Construction of a novel inducible expression vector for Lactococcus lactis M4 and Lactobacillus plantarum Pa21
by: Maidin, Mohd Shawal Thakib, et al.
Published: (2014)
by: Maidin, Mohd Shawal Thakib, et al.
Published: (2014)
Effects of Individual and combined fermentation factors on antimicrobial activity of Nisin by Lactococcus lactis ATCC 11454
by: Mohd Rasid, Nur Hidayah, et al.
Published: (2022)
by: Mohd Rasid, Nur Hidayah, et al.
Published: (2022)
Rerouting the metabolic pathway in Lactococcus lactis ATCC 11454 to improve nisin productivity by disrupting lactate dehydrogenase (ldh)
by: Mohd Rasid, Nur Hidayah, et al.
Published: (2024)
by: Mohd Rasid, Nur Hidayah, et al.
Published: (2024)
Production of alpha-interferon in Lactococcus lactis
by: Bayat, Omid
Published: (2013)
by: Bayat, Omid
Published: (2013)
A process for producing bacteriocin from lactococcus lactis.
by: Foo, Hooi Ling, et al.
Published: (2004)
by: Foo, Hooi Ling, et al.
Published: (2004)
Intracellular production of IFN-alpha 2b in Lactococcus lactis
by: Bayat, Omid, et al.
Published: (2014)
by: Bayat, Omid, et al.
Published: (2014)
K-ras peptide mimotope induces antigen specific Th1 and B-cell immune responses against G12A-mutated K-Ras antigen in balb/c mice
by: Siak, Pui Yan, et al.
Published: (2021)
by: Siak, Pui Yan, et al.
Published: (2021)
Purification and Characterisation of Endopeptidase Produced by Lactococcus Lactis Subsp. Lactis RI 11
by: Woo, Kwan Kit
Published: (2001)
by: Woo, Kwan Kit
Published: (2001)
Construction of an expression vector for Lactococcus lactis based on an indigenous cryptic plasmid
by: Hooi, Wei Yeng, et al.
Published: (2009)
by: Hooi, Wei Yeng, et al.
Published: (2009)
Lactococcus lactis: LAB model organism for bacteria-mediated therapeutic strategies
by: Faudzi, Hanis, et al.
Published: (2018)
by: Faudzi, Hanis, et al.
Published: (2018)
DNA sequence analysis of a small cryptic plasmid from Lactococcus lactis subsp. lactis M14
by: Abdul Rahim, Raha, et al.
Published: (2006)
by: Abdul Rahim, Raha, et al.
Published: (2006)
Stability and expression of K-ras mimotopes in freeze-dried recombinant Lactococcus lactis NZ3900-fermented milk powder during storage in vacuum packaging
by: How, Yu Hsuan, et al.
Published: (2024)
by: How, Yu Hsuan, et al.
Published: (2024)
Cyclodextrin glycosyltransferase biosynthesis by genetically engineered fermentative Lactococcus lactis in aerobic system
by: Amiri, Azin, et al.
Published: (2015)
by: Amiri, Azin, et al.
Published: (2015)
Effects of feeding spraydried metabolite of Lactococcus lactis subsp. lactis – RW18 in postweaning rats
by: Loh, Teck Chwen, et al.
Published: (2008)
by: Loh, Teck Chwen, et al.
Published: (2008)
Oral vaccine of Lactococcus lactis harbouring pandemic H1N1 2009 haemagglutinin1 and nisP anchor fusion protein elevates anti-HA1 sIgA levels in mice
by: Siaw, Stella Xiu Joan, et al.
Published: (2016)
by: Siaw, Stella Xiu Joan, et al.
Published: (2016)
Development of lactococcus lactis vaccine vectors against breast carcinoma
by: Baradaran, Ali
Published: (2015)
by: Baradaran, Ali
Published: (2015)
Engineering Lactococcus lactis as cell factory for the production of plant limonene
by: Shaili, Nurul `Aishah
Published: (2019)
by: Shaili, Nurul `Aishah
Published: (2019)
Cell Surface Display System for Lactococcus Lactis: A Novel Development for Oral Vaccine
by: Abdul Rahim, Raha, et al.
Published: (2005)
by: Abdul Rahim, Raha, et al.
Published: (2005)
Characterisation and molecular cloning of an erythromycin resistance plasmid of Lactococcus lactis isolated from chicken cecum
by: A.R., Raha, et al.
Published: (2002)
by: A.R., Raha, et al.
Published: (2002)
The utilization of Soybean for the production of lactic acid by lactococcus lactis IO-1
by: Angela, Anak Usit.
Published: (2003)
by: Angela, Anak Usit.
Published: (2003)
Construction of vaccine from Lactococcus lactis bacteria using Aeromonas hydrophila virulent Aerolysin gene.
by: H., Sasan, et al.
Published: (2011)
by: H., Sasan, et al.
Published: (2011)
Expression of a thermostable xylanase gene from Bacillus coagulans ST-6 in Lactococcus lactis
by: Abdul Rahim, Raha, et al.
Published: (2006)
by: Abdul Rahim, Raha, et al.
Published: (2006)
Molecular characterization of a recombinant managanese superoxide dismutase from Lactococcus lactis M4
by: Tan, Boon Hooi, et al.
Published: (2014)
by: Tan, Boon Hooi, et al.
Published: (2014)
Similar Items
-
Effect of secretion efficiency of mutant KRAS neoantigen by Lactococcus lactis on the immune response of a mucosal vaccine delivery vehicle targeting colorectal cancer
by: Alias, Nur Aqlili Riana, et al.
Published: (2023) -
Secretion of recombinant xylanase in Lactococcus lactis using signal peptides Usp45 and Spk1
by: Roslan, Abdullah Munir, et al.
Published: (2020) -
Engineering Lactococcus lactis as a cell factory for the production of limonene
by: Nurul ‘Aishah Shaili,, et al.
Published: (2022) -
Engineering Lactococcus lactis as a cell factory for the production of limonene
by: Shaili, Nurul ‘Aishah, et al.
Published: (2022) -
Cloning and in silico characterization of two signal peptides from Pediococcus pentosaceus and their function for the secretion of heterologous protein in Lactococcus lactis.
by: Baradaran, Ali, et al.
Published: (2013)