In Vitro Culture and Symbiotic Relationships of Paphiopedilum Niveum, Taeniophyllum Obtusum and Vanda Hookeriana with Their Associated Fungi

In vitro cultures of three orchid species, namely, Paphiopedilum niveum, Taeniophyllum obtusum and Yanda hookeriana, were initiated from different parts of the plants. Proliferous protocorm-like bodies were obtained from shoot tips and axillary buds of V. hookeriana and shoot tips and in flores...

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Main Author: Irawati
Format: Thesis
Language:English
English
Published: 1987
Subjects:
Online Access:http://psasir.upm.edu.my/id/eprint/10230/
http://psasir.upm.edu.my/id/eprint/10230/1/FP_1987_8.pdf
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author Irawati
author_facet Irawati
author_sort Irawati
building UPM Institutional Repository
collection Online Access
description In vitro cultures of three orchid species, namely, Paphiopedilum niveum, Taeniophyllum obtusum and Yanda hookeriana, were initiated from different parts of the plants. Proliferous protocorm-like bodies were obtained from shoot tips and axillary buds of V. hookeriana and shoot tips and in florescence of T. obtusum. Shoot tips and axillary buds of P. niveum grew into single or multiple plantlets. In florescence culture of T. obtusum developed into multiple branches of in florescences, calli or produced roots. Young leaf tip or leaf base of P. niveum and root of T. obtusum were unable to differentiate into callus. However, culture of young flower bud of P. niveum produced leaf after seven months. High pH of the medium was suitable for T. obtusum cultures. Low concentration of modified Knudson's C medium was preferable to the standard concentration for the culture of P. niveum.
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institution Universiti Putra Malaysia
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language English
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publishDate 1987
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spelling upm-102302024-03-22T02:17:40Z http://psasir.upm.edu.my/id/eprint/10230/ In Vitro Culture and Symbiotic Relationships of Paphiopedilum Niveum, Taeniophyllum Obtusum and Vanda Hookeriana with Their Associated Fungi Irawati In vitro cultures of three orchid species, namely, Paphiopedilum niveum, Taeniophyllum obtusum and Yanda hookeriana, were initiated from different parts of the plants. Proliferous protocorm-like bodies were obtained from shoot tips and axillary buds of V. hookeriana and shoot tips and in florescence of T. obtusum. Shoot tips and axillary buds of P. niveum grew into single or multiple plantlets. In florescence culture of T. obtusum developed into multiple branches of in florescences, calli or produced roots. Young leaf tip or leaf base of P. niveum and root of T. obtusum were unable to differentiate into callus. However, culture of young flower bud of P. niveum produced leaf after seven months. High pH of the medium was suitable for T. obtusum cultures. Low concentration of modified Knudson's C medium was preferable to the standard concentration for the culture of P. niveum. 1987-09 Thesis NonPeerReviewed text en http://psasir.upm.edu.my/id/eprint/10230/1/FP_1987_8.pdf Irawati (1987) In Vitro Culture and Symbiotic Relationships of Paphiopedilum Niveum, Taeniophyllum Obtusum and Vanda Hookeriana with Their Associated Fungi. Doctoral thesis, Universiti Pertanian Malaysia. Orchids - Fertilization in vitro - Case studies English
spellingShingle Orchids - Fertilization in vitro - Case studies
Irawati
In Vitro Culture and Symbiotic Relationships of Paphiopedilum Niveum, Taeniophyllum Obtusum and Vanda Hookeriana with Their Associated Fungi
title In Vitro Culture and Symbiotic Relationships of Paphiopedilum Niveum, Taeniophyllum Obtusum and Vanda Hookeriana with Their Associated Fungi
title_full In Vitro Culture and Symbiotic Relationships of Paphiopedilum Niveum, Taeniophyllum Obtusum and Vanda Hookeriana with Their Associated Fungi
title_fullStr In Vitro Culture and Symbiotic Relationships of Paphiopedilum Niveum, Taeniophyllum Obtusum and Vanda Hookeriana with Their Associated Fungi
title_full_unstemmed In Vitro Culture and Symbiotic Relationships of Paphiopedilum Niveum, Taeniophyllum Obtusum and Vanda Hookeriana with Their Associated Fungi
title_short In Vitro Culture and Symbiotic Relationships of Paphiopedilum Niveum, Taeniophyllum Obtusum and Vanda Hookeriana with Their Associated Fungi
title_sort in vitro culture and symbiotic relationships of paphiopedilum niveum, taeniophyllum obtusum and vanda hookeriana with their associated fungi
topic Orchids - Fertilization in vitro - Case studies
url http://psasir.upm.edu.my/id/eprint/10230/
http://psasir.upm.edu.my/id/eprint/10230/1/FP_1987_8.pdf