Minimally invasive blood collection techniques as a source of gDNA for genetic studies on turtles and tortoises
This technical note aims to describe the venipuncture procedure used to collect blood from Southern River terrapins via the subcarapacial venous plexus (SVP) and jugular vein. On uncooperative terrapins, SVP was applied while the jugular vein was reversed. 1.5 ml blood was preserved in 0.5 ml EDTA a...
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| Format: | Article |
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Iranian society of Ichthyology
2022
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| Online Access: | http://psasir.upm.edu.my/id/eprint/102182/ |
| _version_ | 1848863736683560960 |
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| author | Mohd Salleh, Mohd Hairul Esa, Yuzine |
| author_facet | Mohd Salleh, Mohd Hairul Esa, Yuzine |
| author_sort | Mohd Salleh, Mohd Hairul |
| building | UPM Institutional Repository |
| collection | Online Access |
| description | This technical note aims to describe the venipuncture procedure used to collect blood from Southern River terrapins via the subcarapacial venous plexus (SVP) and jugular vein. On uncooperative terrapins, SVP was applied while the jugular vein was reversed. 1.5 ml blood was preserved in 0.5 ml EDTA and stored at -20°C. ReliaPrepTM Blood genomic DNA Miniprep was used to extract DNA. Thermo ScientificTM NanoDrop 2000c was used to determine the concentrations of extracted DNAs. The greatest concentration of DNA is 136.3 g/L, and the highest purity is 1.90. The treatment is safe, minimally invasive, and effective. |
| first_indexed | 2025-11-15T13:37:40Z |
| format | Article |
| id | upm-102182 |
| institution | Universiti Putra Malaysia |
| institution_category | Local University |
| last_indexed | 2025-11-15T13:37:40Z |
| publishDate | 2022 |
| publisher | Iranian society of Ichthyology |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | upm-1021822023-07-10T01:27:08Z http://psasir.upm.edu.my/id/eprint/102182/ Minimally invasive blood collection techniques as a source of gDNA for genetic studies on turtles and tortoises Mohd Salleh, Mohd Hairul Esa, Yuzine This technical note aims to describe the venipuncture procedure used to collect blood from Southern River terrapins via the subcarapacial venous plexus (SVP) and jugular vein. On uncooperative terrapins, SVP was applied while the jugular vein was reversed. 1.5 ml blood was preserved in 0.5 ml EDTA and stored at -20°C. ReliaPrepTM Blood genomic DNA Miniprep was used to extract DNA. Thermo ScientificTM NanoDrop 2000c was used to determine the concentrations of extracted DNAs. The greatest concentration of DNA is 136.3 g/L, and the highest purity is 1.90. The treatment is safe, minimally invasive, and effective. Iranian society of Ichthyology 2022-06-14 Article PeerReviewed Mohd Salleh, Mohd Hairul and Esa, Yuzine (2022) Minimally invasive blood collection techniques as a source of gDNA for genetic studies on turtles and tortoises. International Journal of Aquatic Biology, 10 (2). 145 - 150. ISSN 2383-0956; ESSN: 2322-5270 https://ij-aquaticbiology.com/index.php/ijab/article/view/1552 |
| spellingShingle | Mohd Salleh, Mohd Hairul Esa, Yuzine Minimally invasive blood collection techniques as a source of gDNA for genetic studies on turtles and tortoises |
| title | Minimally invasive blood collection techniques as a source of gDNA for genetic studies on turtles and tortoises |
| title_full | Minimally invasive blood collection techniques as a source of gDNA for genetic studies on turtles and tortoises |
| title_fullStr | Minimally invasive blood collection techniques as a source of gDNA for genetic studies on turtles and tortoises |
| title_full_unstemmed | Minimally invasive blood collection techniques as a source of gDNA for genetic studies on turtles and tortoises |
| title_short | Minimally invasive blood collection techniques as a source of gDNA for genetic studies on turtles and tortoises |
| title_sort | minimally invasive blood collection techniques as a source of gdna for genetic studies on turtles and tortoises |
| url | http://psasir.upm.edu.my/id/eprint/102182/ http://psasir.upm.edu.my/id/eprint/102182/ |