Magnetic exposure using Samarium Cobalt (SmCO5) increased proliferation and stemness of human Umbilical Cord Mesenchymal Stem Cells (hUC-MSCs)
Despite the extensive reports on the potential hazard of magnetic field (MF) exposures on humans, there are also concurrently reported on the improved proliferative property of stem cells at optimum exposure. However, the effect on mesenchymal stem cells (MSCs) remains unknown. Therefore, we aimed t...
| Main Authors: | , , , , |
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| Format: | Article |
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Nature Publishing Group
2022
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| Online Access: | http://psasir.upm.edu.my/id/eprint/102115/ |
| _version_ | 1848863718347112448 |
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| author | Abdul Hamid, Haslinda Ramasamy, Rajesh Mustafa, Mohd Kamarulzaki Sarmadi, Vahid Hosseinpour Miskon, Azizi |
| author_facet | Abdul Hamid, Haslinda Ramasamy, Rajesh Mustafa, Mohd Kamarulzaki Sarmadi, Vahid Hosseinpour Miskon, Azizi |
| author_sort | Abdul Hamid, Haslinda |
| building | UPM Institutional Repository |
| collection | Online Access |
| description | Despite the extensive reports on the potential hazard of magnetic field (MF) exposures on humans, there are also concurrently reported on the improved proliferative property of stem cells at optimum exposure. However, the effect on mesenchymal stem cells (MSCs) remains unknown. Therefore, we aimed to investigate the impact of induced static MF (SMF) on human umbilical cord-derived mesenchymal stem cells (hUC-MSCs) using Samarium Cobalt (SmCO5). At passage 3, hUC-MSCs (1 × 104) were exposed to 21.6 mT SMF by a direct exposure (DE) showed a significantly higher cell count (p < 0.05) in the growth kinetics assays with the shortest population doubling time relative to indirect exposure and negative control. The DE group was committed into the cell cycle with increased S phase (55.18 ± 1.38%) and G2/M phase (21.75 ± 1.38%) relative to the NC group [S-phase (13.54 ± 2.73%); G2/M phase (8.36 ± 0.28%)]. Although no significant changes were observed in the immunophenotype, the DE group showed an elevated expression of pluripotency-associated markers (OCT4, SOX2, NANOG, and REX1). These results suggest that the MFs could potentially induce proliferation of MSCs, a promising approach to promote stem cells propagation for clinical therapy and research without compromising the stemness of hUC-MSCs. |
| first_indexed | 2025-11-15T13:37:22Z |
| format | Article |
| id | upm-102115 |
| institution | Universiti Putra Malaysia |
| institution_category | Local University |
| last_indexed | 2025-11-15T13:37:22Z |
| publishDate | 2022 |
| publisher | Nature Publishing Group |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | upm-1021152023-06-08T02:11:11Z http://psasir.upm.edu.my/id/eprint/102115/ Magnetic exposure using Samarium Cobalt (SmCO5) increased proliferation and stemness of human Umbilical Cord Mesenchymal Stem Cells (hUC-MSCs) Abdul Hamid, Haslinda Ramasamy, Rajesh Mustafa, Mohd Kamarulzaki Sarmadi, Vahid Hosseinpour Miskon, Azizi Despite the extensive reports on the potential hazard of magnetic field (MF) exposures on humans, there are also concurrently reported on the improved proliferative property of stem cells at optimum exposure. However, the effect on mesenchymal stem cells (MSCs) remains unknown. Therefore, we aimed to investigate the impact of induced static MF (SMF) on human umbilical cord-derived mesenchymal stem cells (hUC-MSCs) using Samarium Cobalt (SmCO5). At passage 3, hUC-MSCs (1 × 104) were exposed to 21.6 mT SMF by a direct exposure (DE) showed a significantly higher cell count (p < 0.05) in the growth kinetics assays with the shortest population doubling time relative to indirect exposure and negative control. The DE group was committed into the cell cycle with increased S phase (55.18 ± 1.38%) and G2/M phase (21.75 ± 1.38%) relative to the NC group [S-phase (13.54 ± 2.73%); G2/M phase (8.36 ± 0.28%)]. Although no significant changes were observed in the immunophenotype, the DE group showed an elevated expression of pluripotency-associated markers (OCT4, SOX2, NANOG, and REX1). These results suggest that the MFs could potentially induce proliferation of MSCs, a promising approach to promote stem cells propagation for clinical therapy and research without compromising the stemness of hUC-MSCs. Nature Publishing Group 2022-05-26 Article PeerReviewed Abdul Hamid, Haslinda and Ramasamy, Rajesh and Mustafa, Mohd Kamarulzaki and Sarmadi, Vahid Hosseinpour and Miskon, Azizi (2022) Magnetic exposure using Samarium Cobalt (SmCO5) increased proliferation and stemness of human Umbilical Cord Mesenchymal Stem Cells (hUC-MSCs). Scientific Reports, 12. art. no. 8904. pp. 1-17. ISSN 2045-2322 https://www.nature.com/articles/s41598-022-12653-z 10.1038/s41598-022-12653-z |
| spellingShingle | Abdul Hamid, Haslinda Ramasamy, Rajesh Mustafa, Mohd Kamarulzaki Sarmadi, Vahid Hosseinpour Miskon, Azizi Magnetic exposure using Samarium Cobalt (SmCO5) increased proliferation and stemness of human Umbilical Cord Mesenchymal Stem Cells (hUC-MSCs) |
| title | Magnetic exposure using Samarium Cobalt (SmCO5) increased proliferation and stemness of human Umbilical Cord Mesenchymal Stem Cells (hUC-MSCs) |
| title_full | Magnetic exposure using Samarium Cobalt (SmCO5) increased proliferation and stemness of human Umbilical Cord Mesenchymal Stem Cells (hUC-MSCs) |
| title_fullStr | Magnetic exposure using Samarium Cobalt (SmCO5) increased proliferation and stemness of human Umbilical Cord Mesenchymal Stem Cells (hUC-MSCs) |
| title_full_unstemmed | Magnetic exposure using Samarium Cobalt (SmCO5) increased proliferation and stemness of human Umbilical Cord Mesenchymal Stem Cells (hUC-MSCs) |
| title_short | Magnetic exposure using Samarium Cobalt (SmCO5) increased proliferation and stemness of human Umbilical Cord Mesenchymal Stem Cells (hUC-MSCs) |
| title_sort | magnetic exposure using samarium cobalt (smco5) increased proliferation and stemness of human umbilical cord mesenchymal stem cells (huc-mscs) |
| url | http://psasir.upm.edu.my/id/eprint/102115/ http://psasir.upm.edu.my/id/eprint/102115/ http://psasir.upm.edu.my/id/eprint/102115/ |