In vitro assessment of multistrain probiotic on its safety, biofilm formation capability, and antimicrobial properties against Aeromonas hydrophila
Probiotics have been increasingly considered an alternative to antibiotics in combating disease outbreaks. Combined probiotics have been studied to possibly harbor synergistic effects that could provide better protection for aquatic species. Three potential probiotics, which had shown in vitro antag...
| Main Authors: | , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Universiti Putra Malaysia
2022
|
| Online Access: | http://psasir.upm.edu.my/id/eprint/102001/ http://psasir.upm.edu.my/id/eprint/102001/1/06%20JTAS-2482-2022.pdf |
| _version_ | 1848863688549728256 |
|---|---|
| author | Wye, Olivia Sze Lee Puvanasundram, Puvaneswari Lim, Keng Chin Karim, Murni |
| author_facet | Wye, Olivia Sze Lee Puvanasundram, Puvaneswari Lim, Keng Chin Karim, Murni |
| author_sort | Wye, Olivia Sze Lee |
| building | UPM Institutional Repository |
| collection | Online Access |
| description | Probiotics have been increasingly considered an alternative to antibiotics in combating disease outbreaks. Combined probiotics have been studied to possibly harbor synergistic effects that could provide better protection for aquatic species. Three potential probiotics, which had shown in vitro antagonism towards Aeromonas hydrophila in this study, were Bacillus amyloliquefaciens (L9, isolated from the blue swimming crab), Lysinibacillus fusiformis (A2, isolated from a microalga), and Enterococcus hirae (LAB3, isolated from the Asian seabass) were combined into a probiotic mixture. The probiotic mixture produced significantly higher biofilm (P < 0.05) (2.441 ± 0.346) than A. hydrophila (0.578 ± 0.124) during 24-h and showed a continuous increase in production at 48-h and 72-h time intervals, respectively. Furthermore, no hemolytic action was observed when the probiotic mixture was streaked on sheep blood agar (5%), whereas A. hydrophila presented α-hemolysis. The lowest concentration of the probiotic mixture (107 CFU mL-1) significantly inhibited (P < 0.05) the growth of A. hydrophila at 106 CFU mL-1 after 24 h of incubation, where bacterial count in the treatment was 6.595 ± 0.218 CFU mL-1, which was significantly lower (P < 0.05) than the control (7.247 ± 0.061 CFU) mL-1. Significant reduction (P < 0.05) in Aeromonas count from 7.532 ± 0.026 CFU mL-1 to 6.883 ± 0.015 CFU mL-1 was observed at 12 hours of co-incubation. Hence, this research suggests that the probiotic mixture of L9, A2, and LAB3 potentially confers protection against A. hydrophila infection due to their characteristics meeting the criteria of probiotics. |
| first_indexed | 2025-11-15T13:36:54Z |
| format | Article |
| id | upm-102001 |
| institution | Universiti Putra Malaysia |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-15T13:36:54Z |
| publishDate | 2022 |
| publisher | Universiti Putra Malaysia |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | upm-1020012023-09-23T00:00:57Z http://psasir.upm.edu.my/id/eprint/102001/ In vitro assessment of multistrain probiotic on its safety, biofilm formation capability, and antimicrobial properties against Aeromonas hydrophila Wye, Olivia Sze Lee Puvanasundram, Puvaneswari Lim, Keng Chin Karim, Murni Probiotics have been increasingly considered an alternative to antibiotics in combating disease outbreaks. Combined probiotics have been studied to possibly harbor synergistic effects that could provide better protection for aquatic species. Three potential probiotics, which had shown in vitro antagonism towards Aeromonas hydrophila in this study, were Bacillus amyloliquefaciens (L9, isolated from the blue swimming crab), Lysinibacillus fusiformis (A2, isolated from a microalga), and Enterococcus hirae (LAB3, isolated from the Asian seabass) were combined into a probiotic mixture. The probiotic mixture produced significantly higher biofilm (P < 0.05) (2.441 ± 0.346) than A. hydrophila (0.578 ± 0.124) during 24-h and showed a continuous increase in production at 48-h and 72-h time intervals, respectively. Furthermore, no hemolytic action was observed when the probiotic mixture was streaked on sheep blood agar (5%), whereas A. hydrophila presented α-hemolysis. The lowest concentration of the probiotic mixture (107 CFU mL-1) significantly inhibited (P < 0.05) the growth of A. hydrophila at 106 CFU mL-1 after 24 h of incubation, where bacterial count in the treatment was 6.595 ± 0.218 CFU mL-1, which was significantly lower (P < 0.05) than the control (7.247 ± 0.061 CFU) mL-1. Significant reduction (P < 0.05) in Aeromonas count from 7.532 ± 0.026 CFU mL-1 to 6.883 ± 0.015 CFU mL-1 was observed at 12 hours of co-incubation. Hence, this research suggests that the probiotic mixture of L9, A2, and LAB3 potentially confers protection against A. hydrophila infection due to their characteristics meeting the criteria of probiotics. Universiti Putra Malaysia 2022-11-04 Article PeerReviewed text en http://psasir.upm.edu.my/id/eprint/102001/1/06%20JTAS-2482-2022.pdf Wye, Olivia Sze Lee and Puvanasundram, Puvaneswari and Lim, Keng Chin and Karim, Murni (2022) In vitro assessment of multistrain probiotic on its safety, biofilm formation capability, and antimicrobial properties against Aeromonas hydrophila. Pertanika Journal of Tropical Agricultural Science, 45 (4). 943 - 959. ISSN 1511-3701; ESSN: 2231-8542 http://www.pertanika.upm.edu.my/pjtas/browse/regular-issue?article=JTAS-2482-2022 10.47836/pjtas.45.4.06 |
| spellingShingle | Wye, Olivia Sze Lee Puvanasundram, Puvaneswari Lim, Keng Chin Karim, Murni In vitro assessment of multistrain probiotic on its safety, biofilm formation capability, and antimicrobial properties against Aeromonas hydrophila |
| title | In vitro assessment of multistrain probiotic on its safety, biofilm formation capability, and antimicrobial properties against Aeromonas hydrophila |
| title_full | In vitro assessment of multistrain probiotic on its safety, biofilm formation capability, and antimicrobial properties against Aeromonas hydrophila |
| title_fullStr | In vitro assessment of multistrain probiotic on its safety, biofilm formation capability, and antimicrobial properties against Aeromonas hydrophila |
| title_full_unstemmed | In vitro assessment of multistrain probiotic on its safety, biofilm formation capability, and antimicrobial properties against Aeromonas hydrophila |
| title_short | In vitro assessment of multistrain probiotic on its safety, biofilm formation capability, and antimicrobial properties against Aeromonas hydrophila |
| title_sort | in vitro assessment of multistrain probiotic on its safety, biofilm formation capability, and antimicrobial properties against aeromonas hydrophila |
| url | http://psasir.upm.edu.my/id/eprint/102001/ http://psasir.upm.edu.my/id/eprint/102001/ http://psasir.upm.edu.my/id/eprint/102001/ http://psasir.upm.edu.my/id/eprint/102001/1/06%20JTAS-2482-2022.pdf |