In vitro anti-diabetic activities and UHPLC-ESI-MS/MS profile of Muntingia calabura leaves extract
Anti-diabetic compounds from natural sources are now being preferred to prevent or treat diabetes due to adverse effects of synthetic drugs. The decoction of Muntingia calabura leaves was traditionally consumed for diabetes treatment. However, there has not been any published data currently availabl...
| Main Authors: | , , , |
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| Format: | Article |
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Multidisciplinary Digital Publishing Institute
2022
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| Online Access: | http://psasir.upm.edu.my/id/eprint/102000/ |
| _version_ | 1848863688295972864 |
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| author | Zolkeflee, Nur Khaleeda Zulaikha Ramli, Nurul Shazini Azlan, Azrina Abas, Faridah |
| author_facet | Zolkeflee, Nur Khaleeda Zulaikha Ramli, Nurul Shazini Azlan, Azrina Abas, Faridah |
| author_sort | Zolkeflee, Nur Khaleeda Zulaikha |
| building | UPM Institutional Repository |
| collection | Online Access |
| description | Anti-diabetic compounds from natural sources are now being preferred to prevent or treat diabetes due to adverse effects of synthetic drugs. The decoction of Muntingia calabura leaves was traditionally consumed for diabetes treatment. However, there has not been any published data currently available on the processing effects on this plant’s biological activity and phytochemical profile. Therefore, this study aims to evaluate the effect of three drying methods (freeze-drying (FD), air-drying (AD), and oven-drying (OD)) and ethanol:water ratios (0, 50, and 100%) on in vitro anti-diabetic activities of M. calabura leaves. In addition, an ultrahigh-performance-liquid chromatography–electrospray ionization tandem mass spectrometry (UHPLC-ESI-MS/MS) method was used to characterize the metabolites in the active extract. The FD M. calabura leaves, extracted with 50% ethanol, is the most active extract that exhibits a high α-glucosidase and α-amylase inhibitory activities with IC50 values of 0.46 ± 0.05 and 26.39 ± 3.93 µg/mL, respectively. Sixty-one compounds were tentatively identified by using UHPLC-ESI-MS/MS from the most active extract. Quantitative analysis, by using UHPLC, revealed that geniposide, daidzein, quercitrin, 6-hydroxyflavanone, kaempferol, and formononetin were predominant compounds identified from the active extract. The results have laid down preliminary steps toward developing M. calabura leaves extract as a potential source of bioactive compounds for diabetic treatment. |
| first_indexed | 2025-11-15T13:36:54Z |
| format | Article |
| id | upm-102000 |
| institution | Universiti Putra Malaysia |
| institution_category | Local University |
| last_indexed | 2025-11-15T13:36:54Z |
| publishDate | 2022 |
| publisher | Multidisciplinary Digital Publishing Institute |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | upm-1020002023-06-16T04:04:53Z http://psasir.upm.edu.my/id/eprint/102000/ In vitro anti-diabetic activities and UHPLC-ESI-MS/MS profile of Muntingia calabura leaves extract Zolkeflee, Nur Khaleeda Zulaikha Ramli, Nurul Shazini Azlan, Azrina Abas, Faridah Anti-diabetic compounds from natural sources are now being preferred to prevent or treat diabetes due to adverse effects of synthetic drugs. The decoction of Muntingia calabura leaves was traditionally consumed for diabetes treatment. However, there has not been any published data currently available on the processing effects on this plant’s biological activity and phytochemical profile. Therefore, this study aims to evaluate the effect of three drying methods (freeze-drying (FD), air-drying (AD), and oven-drying (OD)) and ethanol:water ratios (0, 50, and 100%) on in vitro anti-diabetic activities of M. calabura leaves. In addition, an ultrahigh-performance-liquid chromatography–electrospray ionization tandem mass spectrometry (UHPLC-ESI-MS/MS) method was used to characterize the metabolites in the active extract. The FD M. calabura leaves, extracted with 50% ethanol, is the most active extract that exhibits a high α-glucosidase and α-amylase inhibitory activities with IC50 values of 0.46 ± 0.05 and 26.39 ± 3.93 µg/mL, respectively. Sixty-one compounds were tentatively identified by using UHPLC-ESI-MS/MS from the most active extract. Quantitative analysis, by using UHPLC, revealed that geniposide, daidzein, quercitrin, 6-hydroxyflavanone, kaempferol, and formononetin were predominant compounds identified from the active extract. The results have laid down preliminary steps toward developing M. calabura leaves extract as a potential source of bioactive compounds for diabetic treatment. Multidisciplinary Digital Publishing Institute 2022-01-04 Article PeerReviewed Zolkeflee, Nur Khaleeda Zulaikha and Ramli, Nurul Shazini and Azlan, Azrina and Abas, Faridah (2022) In vitro anti-diabetic activities and UHPLC-ESI-MS/MS profile of Muntingia calabura leaves extract. Molecules, 27 (1). art. no. 287. pp. 1-21. ISSN 1431-5165; ESSN: 1420-3049 https://www.mdpi.com/1420-3049/27/1/287 10.3390/molecules27010287 |
| spellingShingle | Zolkeflee, Nur Khaleeda Zulaikha Ramli, Nurul Shazini Azlan, Azrina Abas, Faridah In vitro anti-diabetic activities and UHPLC-ESI-MS/MS profile of Muntingia calabura leaves extract |
| title | In vitro anti-diabetic activities and UHPLC-ESI-MS/MS profile of Muntingia calabura leaves extract |
| title_full | In vitro anti-diabetic activities and UHPLC-ESI-MS/MS profile of Muntingia calabura leaves extract |
| title_fullStr | In vitro anti-diabetic activities and UHPLC-ESI-MS/MS profile of Muntingia calabura leaves extract |
| title_full_unstemmed | In vitro anti-diabetic activities and UHPLC-ESI-MS/MS profile of Muntingia calabura leaves extract |
| title_short | In vitro anti-diabetic activities and UHPLC-ESI-MS/MS profile of Muntingia calabura leaves extract |
| title_sort | in vitro anti-diabetic activities and uhplc-esi-ms/ms profile of muntingia calabura leaves extract |
| url | http://psasir.upm.edu.my/id/eprint/102000/ http://psasir.upm.edu.my/id/eprint/102000/ http://psasir.upm.edu.my/id/eprint/102000/ |