Inhibition of Corynebacterium pseudotuberculosis biofilm by DNA synthesis and protein synthesis inhibitors
Corynebacterium pseudotuberculosis represents the causative factor of caseous lymphadenitis, a ruminant disease that contributes to major economic loss in most sheep farming countries. The present study was performed to determine the efficacy of DNA synthesis (nalidixic acid) and protein synthesis i...
| Main Authors: | , , , , |
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| Format: | Article |
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Universiti Malaysia Terengganu
2022
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| Online Access: | http://psasir.upm.edu.my/id/eprint/101924/ |
| _version_ | 1848863668645658624 |
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| author | Rashid, Syaida Anati Abd Yaacob, Mohamad Fakhri Aazmi, Mohd Shafiq Jesse, Faez Firdaus Abdullah Raja Yahya, Mohd Fakharul Zaman |
| author_facet | Rashid, Syaida Anati Abd Yaacob, Mohamad Fakhri Aazmi, Mohd Shafiq Jesse, Faez Firdaus Abdullah Raja Yahya, Mohd Fakharul Zaman |
| author_sort | Rashid, Syaida Anati Abd |
| building | UPM Institutional Repository |
| collection | Online Access |
| description | Corynebacterium pseudotuberculosis represents the causative factor of caseous lymphadenitis, a ruminant disease that contributes to major economic loss in most sheep farming countries. The present study was performed to determine the efficacy of DNA synthesis (nalidixic acid) and protein synthesis inhibitors (streptomycin and tetracycline) on C. pseudotuberculosis biofilm. Biofilm of C. pseudotuberculosis clinical isolate was developed in the flat-bottom microplate. Field-emission scanning electron microscope and resazurin assay were used to investigate the structure and antimicrobial susceptibility of C. pseudotuberculosis biofilm, respectively. All test inhibitors were evaluated in the range between 3.125 μg/ml and 100 μg/ml. Results demonstrated that C. pseudotuberculosis biofilm formed a three dimensional and heterogeneous structure on the surface. The highest biofilm percentage inhibition shown by nalidixic acid, streptomycin and tetracycline were 71.57%, 87.44% and 74.73%, respectively. |
| first_indexed | 2025-11-15T13:36:35Z |
| format | Article |
| id | upm-101924 |
| institution | Universiti Putra Malaysia |
| institution_category | Local University |
| last_indexed | 2025-11-15T13:36:35Z |
| publishDate | 2022 |
| publisher | Universiti Malaysia Terengganu |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | upm-1019242024-05-09T03:19:13Z http://psasir.upm.edu.my/id/eprint/101924/ Inhibition of Corynebacterium pseudotuberculosis biofilm by DNA synthesis and protein synthesis inhibitors Rashid, Syaida Anati Abd Yaacob, Mohamad Fakhri Aazmi, Mohd Shafiq Jesse, Faez Firdaus Abdullah Raja Yahya, Mohd Fakharul Zaman Corynebacterium pseudotuberculosis represents the causative factor of caseous lymphadenitis, a ruminant disease that contributes to major economic loss in most sheep farming countries. The present study was performed to determine the efficacy of DNA synthesis (nalidixic acid) and protein synthesis inhibitors (streptomycin and tetracycline) on C. pseudotuberculosis biofilm. Biofilm of C. pseudotuberculosis clinical isolate was developed in the flat-bottom microplate. Field-emission scanning electron microscope and resazurin assay were used to investigate the structure and antimicrobial susceptibility of C. pseudotuberculosis biofilm, respectively. All test inhibitors were evaluated in the range between 3.125 μg/ml and 100 μg/ml. Results demonstrated that C. pseudotuberculosis biofilm formed a three dimensional and heterogeneous structure on the surface. The highest biofilm percentage inhibition shown by nalidixic acid, streptomycin and tetracycline were 71.57%, 87.44% and 74.73%, respectively. Universiti Malaysia Terengganu 2022-04 Article PeerReviewed Rashid, Syaida Anati Abd and Yaacob, Mohamad Fakhri and Aazmi, Mohd Shafiq and Jesse, Faez Firdaus Abdullah and Raja Yahya, Mohd Fakharul Zaman (2022) Inhibition of Corynebacterium pseudotuberculosis biofilm by DNA synthesis and protein synthesis inhibitors. Journal of Sustainability Science and Management, 17 (4). 49 - 56. ISSN 1823-8556; ESSN: 2672-7226 https://jssm.umt.edu.my/archive/volume-17-number-4-april-2022-2/ 10.46754/jssm.2022.4.004 |
| spellingShingle | Rashid, Syaida Anati Abd Yaacob, Mohamad Fakhri Aazmi, Mohd Shafiq Jesse, Faez Firdaus Abdullah Raja Yahya, Mohd Fakharul Zaman Inhibition of Corynebacterium pseudotuberculosis biofilm by DNA synthesis and protein synthesis inhibitors |
| title | Inhibition of Corynebacterium pseudotuberculosis biofilm by DNA synthesis and protein synthesis inhibitors |
| title_full | Inhibition of Corynebacterium pseudotuberculosis biofilm by DNA synthesis and protein synthesis inhibitors |
| title_fullStr | Inhibition of Corynebacterium pseudotuberculosis biofilm by DNA synthesis and protein synthesis inhibitors |
| title_full_unstemmed | Inhibition of Corynebacterium pseudotuberculosis biofilm by DNA synthesis and protein synthesis inhibitors |
| title_short | Inhibition of Corynebacterium pseudotuberculosis biofilm by DNA synthesis and protein synthesis inhibitors |
| title_sort | inhibition of corynebacterium pseudotuberculosis biofilm by dna synthesis and protein synthesis inhibitors |
| url | http://psasir.upm.edu.my/id/eprint/101924/ http://psasir.upm.edu.my/id/eprint/101924/ http://psasir.upm.edu.my/id/eprint/101924/ |