Metabolic pathway of phenol degradation of a cold-adapted antarctic bacteria, Arthrobacter sp.
Phenol is an important pollutant widely discharged as a component of hydrocarbon fuels, but its degradation in cold regions is challenging due to the harsh environmental conditions. To date, there is little information available concerning the capability for phenol biodegradation by indigenous Antar...
| Main Authors: | , , , , , , , , |
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| Format: | Article |
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Multidisciplinary Digital Publishing Institute
2022
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| Online Access: | http://psasir.upm.edu.my/id/eprint/101850/ |
| _version_ | 1848863647293505536 |
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| author | Lee, Gillian Li Yin Zakaria, Nur Nadhirah Futamata, Hiroyuki Suzuki, Kenshi Zulkharnain, Azham Shaharuddin, Noor Azmi Convey, Peter Mohd Zahri, Khadijah Nabilah Ahmad, Siti Aqlima |
| author_facet | Lee, Gillian Li Yin Zakaria, Nur Nadhirah Futamata, Hiroyuki Suzuki, Kenshi Zulkharnain, Azham Shaharuddin, Noor Azmi Convey, Peter Mohd Zahri, Khadijah Nabilah Ahmad, Siti Aqlima |
| author_sort | Lee, Gillian Li Yin |
| building | UPM Institutional Repository |
| collection | Online Access |
| description | Phenol is an important pollutant widely discharged as a component of hydrocarbon fuels, but its degradation in cold regions is challenging due to the harsh environmental conditions. To date, there is little information available concerning the capability for phenol biodegradation by indigenous Antarctic bacteria. In this study, enzyme activities and genes encoding phenol degradative enzymes identified using whole genome sequencing (WGS) were investigated to determine the pathway(s) of phenol degradation of Arthrobacter sp. strains AQ5-05 and AQ5-06, originally isolated from Antarctica. Complete phenol degradative genes involved only in the ortho-cleavage were detected in both strains. This was validated using assays of the enzymes catechol 1,2-dioxygenase and catechol 2,3-dioxygenase, which indicated the activity of only catechol 1,2-dioxygenase in both strains, in agreement with the results from the WGS. Both strains were psychrotolerant with the optimum temperature for phenol degradation, being between 10 and 15 °C. This study suggests the potential use of cold-adapted bacteria in the bioremediation of phenol pollution in cold environments. |
| first_indexed | 2025-11-15T13:36:14Z |
| format | Article |
| id | upm-101850 |
| institution | Universiti Putra Malaysia |
| institution_category | Local University |
| last_indexed | 2025-11-15T13:36:14Z |
| publishDate | 2022 |
| publisher | Multidisciplinary Digital Publishing Institute |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | upm-1018502023-06-17T22:48:41Z http://psasir.upm.edu.my/id/eprint/101850/ Metabolic pathway of phenol degradation of a cold-adapted antarctic bacteria, Arthrobacter sp. Lee, Gillian Li Yin Zakaria, Nur Nadhirah Futamata, Hiroyuki Suzuki, Kenshi Zulkharnain, Azham Shaharuddin, Noor Azmi Convey, Peter Mohd Zahri, Khadijah Nabilah Ahmad, Siti Aqlima Phenol is an important pollutant widely discharged as a component of hydrocarbon fuels, but its degradation in cold regions is challenging due to the harsh environmental conditions. To date, there is little information available concerning the capability for phenol biodegradation by indigenous Antarctic bacteria. In this study, enzyme activities and genes encoding phenol degradative enzymes identified using whole genome sequencing (WGS) were investigated to determine the pathway(s) of phenol degradation of Arthrobacter sp. strains AQ5-05 and AQ5-06, originally isolated from Antarctica. Complete phenol degradative genes involved only in the ortho-cleavage were detected in both strains. This was validated using assays of the enzymes catechol 1,2-dioxygenase and catechol 2,3-dioxygenase, which indicated the activity of only catechol 1,2-dioxygenase in both strains, in agreement with the results from the WGS. Both strains were psychrotolerant with the optimum temperature for phenol degradation, being between 10 and 15 °C. This study suggests the potential use of cold-adapted bacteria in the bioremediation of phenol pollution in cold environments. Multidisciplinary Digital Publishing Institute 2022-11-12 Article PeerReviewed Lee, Gillian Li Yin and Zakaria, Nur Nadhirah and Futamata, Hiroyuki and Suzuki, Kenshi and Zulkharnain, Azham and Shaharuddin, Noor Azmi and Convey, Peter and Mohd Zahri, Khadijah Nabilah and Ahmad, Siti Aqlima (2022) Metabolic pathway of phenol degradation of a cold-adapted antarctic bacteria, Arthrobacter sp. Catalysts, 12 (11). art. no. 1422. pp. 1-16. ISSN 2073-4344 https://www.mdpi.com/2073-4344/12/11/1422 10.3390/catal12111422 |
| spellingShingle | Lee, Gillian Li Yin Zakaria, Nur Nadhirah Futamata, Hiroyuki Suzuki, Kenshi Zulkharnain, Azham Shaharuddin, Noor Azmi Convey, Peter Mohd Zahri, Khadijah Nabilah Ahmad, Siti Aqlima Metabolic pathway of phenol degradation of a cold-adapted antarctic bacteria, Arthrobacter sp. |
| title | Metabolic pathway of phenol degradation of a cold-adapted antarctic bacteria, Arthrobacter sp. |
| title_full | Metabolic pathway of phenol degradation of a cold-adapted antarctic bacteria, Arthrobacter sp. |
| title_fullStr | Metabolic pathway of phenol degradation of a cold-adapted antarctic bacteria, Arthrobacter sp. |
| title_full_unstemmed | Metabolic pathway of phenol degradation of a cold-adapted antarctic bacteria, Arthrobacter sp. |
| title_short | Metabolic pathway of phenol degradation of a cold-adapted antarctic bacteria, Arthrobacter sp. |
| title_sort | metabolic pathway of phenol degradation of a cold-adapted antarctic bacteria, arthrobacter sp. |
| url | http://psasir.upm.edu.my/id/eprint/101850/ http://psasir.upm.edu.my/id/eprint/101850/ http://psasir.upm.edu.my/id/eprint/101850/ |