Human umbilical cord mesenchymal stem cell-derived small extracellular vesicles ameliorated insulin resistance in type 2 diabetes mellitus rats
Human umbilical cord mesenchymal stem cell-derived small extracellular vesicle (hUC-MSCs-sEVs) therapy has shown promising results to treat diabetes mellitus in preclinical studies. However, the dosage of MSCs-sEVs in animal studies, up to 10 mg/kg, was considered high and may be impractical for fut...
| Main Authors: | , , , , , , , , , |
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| Format: | Article |
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Multidisciplinary Digital Publishing Institute
2022
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| Online Access: | http://psasir.upm.edu.my/id/eprint/101745/ |
| _version_ | 1848863621496438784 |
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| author | Yap, Seng Kar Tan, Kian Leong Abd Rahaman, Nor Yasmin Saulol Hamid, Nur Fazila Ooi, Der Jiun Tor, Yin Sim Daniel Looi, Qi Hao Li, Kar Stella Tan How, Chee Wun Foo, Jhi Biau |
| author_facet | Yap, Seng Kar Tan, Kian Leong Abd Rahaman, Nor Yasmin Saulol Hamid, Nur Fazila Ooi, Der Jiun Tor, Yin Sim Daniel Looi, Qi Hao Li, Kar Stella Tan How, Chee Wun Foo, Jhi Biau |
| author_sort | Yap, Seng Kar |
| building | UPM Institutional Repository |
| collection | Online Access |
| description | Human umbilical cord mesenchymal stem cell-derived small extracellular vesicle (hUC-MSCs-sEVs) therapy has shown promising results to treat diabetes mellitus in preclinical studies. However, the dosage of MSCs-sEVs in animal studies, up to 10 mg/kg, was considered high and may be impractical for future clinical application. This study aims to investigate the efficacy of low-dose hUC-MSCs-sEVs treatment on human skeletal muscle cells (HSkMCs) and type 2 diabetes mellitus (T2DM) rats. Treatment with hUC-MSCs-sEVs up to 100 μg/mL for 48 h showed no significant cytotoxicity. Interestingly, 20 μg/mL of hUC-MSCs-sEVs-treated HSkMCs increased glucose uptake by 80–90% compared to untreated cells. The hUC-MSCs-sEVs treatment at 1 mg/kg improved glucose tolerance in T2DM rats and showed a protective effect on complete blood count. Moreover, an improvement in serum HbA1c was observed in diabetic rats treated with 0.5 and 1 mg/kg of hUC-MSCs-sEVs, and hUC-MSCs. The biochemical tests of hUC-MSCs-sEVs treatment groups showed no significant creatinine changes, elevated alanine aminotransferase (ALT) and alkaline phosphatase (ALP) levels compared to the normal group. Histological analysis revealed that hUC-MSCs-sEVs relieved the structural damage to the pancreas, kidney and liver. The findings suggest that hUC-MSCs-sEVs could ameliorate insulin resistance and exert protective effects on T2DM rats. Therefore, hUC-MSCs-sEVs could serve as a potential therapy for diabetes mellitus. |
| first_indexed | 2025-11-15T13:35:50Z |
| format | Article |
| id | upm-101745 |
| institution | Universiti Putra Malaysia |
| institution_category | Local University |
| last_indexed | 2025-11-15T13:35:50Z |
| publishDate | 2022 |
| publisher | Multidisciplinary Digital Publishing Institute |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | upm-1017452023-06-16T20:18:49Z http://psasir.upm.edu.my/id/eprint/101745/ Human umbilical cord mesenchymal stem cell-derived small extracellular vesicles ameliorated insulin resistance in type 2 diabetes mellitus rats Yap, Seng Kar Tan, Kian Leong Abd Rahaman, Nor Yasmin Saulol Hamid, Nur Fazila Ooi, Der Jiun Tor, Yin Sim Daniel Looi, Qi Hao Li, Kar Stella Tan How, Chee Wun Foo, Jhi Biau Human umbilical cord mesenchymal stem cell-derived small extracellular vesicle (hUC-MSCs-sEVs) therapy has shown promising results to treat diabetes mellitus in preclinical studies. However, the dosage of MSCs-sEVs in animal studies, up to 10 mg/kg, was considered high and may be impractical for future clinical application. This study aims to investigate the efficacy of low-dose hUC-MSCs-sEVs treatment on human skeletal muscle cells (HSkMCs) and type 2 diabetes mellitus (T2DM) rats. Treatment with hUC-MSCs-sEVs up to 100 μg/mL for 48 h showed no significant cytotoxicity. Interestingly, 20 μg/mL of hUC-MSCs-sEVs-treated HSkMCs increased glucose uptake by 80–90% compared to untreated cells. The hUC-MSCs-sEVs treatment at 1 mg/kg improved glucose tolerance in T2DM rats and showed a protective effect on complete blood count. Moreover, an improvement in serum HbA1c was observed in diabetic rats treated with 0.5 and 1 mg/kg of hUC-MSCs-sEVs, and hUC-MSCs. The biochemical tests of hUC-MSCs-sEVs treatment groups showed no significant creatinine changes, elevated alanine aminotransferase (ALT) and alkaline phosphatase (ALP) levels compared to the normal group. Histological analysis revealed that hUC-MSCs-sEVs relieved the structural damage to the pancreas, kidney and liver. The findings suggest that hUC-MSCs-sEVs could ameliorate insulin resistance and exert protective effects on T2DM rats. Therefore, hUC-MSCs-sEVs could serve as a potential therapy for diabetes mellitus. Multidisciplinary Digital Publishing Institute 2022-03-16 Article PeerReviewed Yap, Seng Kar and Tan, Kian Leong and Abd Rahaman, Nor Yasmin and Saulol Hamid, Nur Fazila and Ooi, Der Jiun and Tor, Yin Sim and Daniel Looi, Qi Hao and Li, Kar Stella Tan and How, Chee Wun and Foo, Jhi Biau (2022) Human umbilical cord mesenchymal stem cell-derived small extracellular vesicles ameliorated insulin resistance in type 2 diabetes mellitus rats. Pharmaceutics, 14 (3). art. no. 649. pp. 1-21. ISSN 1999-4923 https://www.mdpi.com/1999-4923/14/3/649 10.3390/pharmaceutics14030649 |
| spellingShingle | Yap, Seng Kar Tan, Kian Leong Abd Rahaman, Nor Yasmin Saulol Hamid, Nur Fazila Ooi, Der Jiun Tor, Yin Sim Daniel Looi, Qi Hao Li, Kar Stella Tan How, Chee Wun Foo, Jhi Biau Human umbilical cord mesenchymal stem cell-derived small extracellular vesicles ameliorated insulin resistance in type 2 diabetes mellitus rats |
| title | Human umbilical cord mesenchymal stem cell-derived small extracellular vesicles ameliorated insulin resistance in type 2 diabetes mellitus rats |
| title_full | Human umbilical cord mesenchymal stem cell-derived small extracellular vesicles ameliorated insulin resistance in type 2 diabetes mellitus rats |
| title_fullStr | Human umbilical cord mesenchymal stem cell-derived small extracellular vesicles ameliorated insulin resistance in type 2 diabetes mellitus rats |
| title_full_unstemmed | Human umbilical cord mesenchymal stem cell-derived small extracellular vesicles ameliorated insulin resistance in type 2 diabetes mellitus rats |
| title_short | Human umbilical cord mesenchymal stem cell-derived small extracellular vesicles ameliorated insulin resistance in type 2 diabetes mellitus rats |
| title_sort | human umbilical cord mesenchymal stem cell-derived small extracellular vesicles ameliorated insulin resistance in type 2 diabetes mellitus rats |
| url | http://psasir.upm.edu.my/id/eprint/101745/ http://psasir.upm.edu.my/id/eprint/101745/ http://psasir.upm.edu.my/id/eprint/101745/ |