Evaluation of three Bacillus spp. isolated from the gut of giant freshwater prawn as potential probiotics against pathogens causing Vibriosis and Aeromonosis
Pathogens from the Vibrio and Aeromonas genera often cause detrimental effects to the aquaculture sector. Previously, antibiotics was used to resolve the infections, but this caused the spread of antibiotic resistant bacteria and antibiotic resistance genes into the environment. As an approach to ad...
| Main Authors: | , , , , , , , |
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| Format: | Article |
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Elsevier
2022
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| Online Access: | http://psasir.upm.edu.my/id/eprint/101345/ |
| _version_ | 1848863542128672768 |
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| author | Sam-on, Muhamad Firdaus Syahmi Mustafa, Shuhaimi Yusof, Mohd Termizi Mohd Hashim, Amalia Abbasiliasi, Sahar Zulkifly, Shahrizim Jahari, Muhamad Amin Roslan, Muhamad Akhmal Hakim |
| author_facet | Sam-on, Muhamad Firdaus Syahmi Mustafa, Shuhaimi Yusof, Mohd Termizi Mohd Hashim, Amalia Abbasiliasi, Sahar Zulkifly, Shahrizim Jahari, Muhamad Amin Roslan, Muhamad Akhmal Hakim |
| author_sort | Sam-on, Muhamad Firdaus Syahmi |
| building | UPM Institutional Repository |
| collection | Online Access |
| description | Pathogens from the Vibrio and Aeromonas genera often cause detrimental effects to the aquaculture sector. Previously, antibiotics was used to resolve the infections, but this caused the spread of antibiotic resistant bacteria and antibiotic resistance genes into the environment. As an approach to address this issue, probiotic bacteria were introduced to improve the hosts' microbiome, disease protection, health condition, growth efficiency, feed consumption, stress response and general vigour. However, reports showed that some commercially available probiotics were restricted to a small number of microbial species and there are inconsistencies concerning its effectiveness. Hence, the aim of this study was to isolate and evaluate new Bacillus spp. from the gut of giant freshwater prawn as potential probiotics. Three Bacillus spp. isolates, Bacillus subtilis FS6 (MZ960135), Bacillus velezensis FS26 (MZ960133) and Bacillus pumilus FS97 (MZ960136) were characterised, and in vitro testing showed good probiotic properties which can help in dealing with diseases in aquaculture. Among the Bacillus spp., Bacillus velezensis FS26 showed higher antimicrobial activity towards Aeromonas hydrophila LMG 13658 and Aeromonas veronii clone DK-A. veronii-27 at 23.7 mm and 25 mm, respectively. Bacillus subtilis FS6 and Bacillus velezensis FS26 resulted in good adherence to both xylene and chloroform hydrocarbons. The Bacillus spp. isolated displayed high survivability towards 0.3% bile salt and exhibited amylase, protease, and lipase activities. Thus, the isolated Bacillus spp. are considered safe based on the sensitivity analysis towards antibiotics and γ-haemolytic activity. |
| first_indexed | 2025-11-15T13:34:34Z |
| format | Article |
| id | upm-101345 |
| institution | Universiti Putra Malaysia |
| institution_category | Local University |
| last_indexed | 2025-11-15T13:34:34Z |
| publishDate | 2022 |
| publisher | Elsevier |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | upm-1013452023-08-15T04:19:59Z http://psasir.upm.edu.my/id/eprint/101345/ Evaluation of three Bacillus spp. isolated from the gut of giant freshwater prawn as potential probiotics against pathogens causing Vibriosis and Aeromonosis Sam-on, Muhamad Firdaus Syahmi Mustafa, Shuhaimi Yusof, Mohd Termizi Mohd Hashim, Amalia Abbasiliasi, Sahar Zulkifly, Shahrizim Jahari, Muhamad Amin Roslan, Muhamad Akhmal Hakim Pathogens from the Vibrio and Aeromonas genera often cause detrimental effects to the aquaculture sector. Previously, antibiotics was used to resolve the infections, but this caused the spread of antibiotic resistant bacteria and antibiotic resistance genes into the environment. As an approach to address this issue, probiotic bacteria were introduced to improve the hosts' microbiome, disease protection, health condition, growth efficiency, feed consumption, stress response and general vigour. However, reports showed that some commercially available probiotics were restricted to a small number of microbial species and there are inconsistencies concerning its effectiveness. Hence, the aim of this study was to isolate and evaluate new Bacillus spp. from the gut of giant freshwater prawn as potential probiotics. Three Bacillus spp. isolates, Bacillus subtilis FS6 (MZ960135), Bacillus velezensis FS26 (MZ960133) and Bacillus pumilus FS97 (MZ960136) were characterised, and in vitro testing showed good probiotic properties which can help in dealing with diseases in aquaculture. Among the Bacillus spp., Bacillus velezensis FS26 showed higher antimicrobial activity towards Aeromonas hydrophila LMG 13658 and Aeromonas veronii clone DK-A. veronii-27 at 23.7 mm and 25 mm, respectively. Bacillus subtilis FS6 and Bacillus velezensis FS26 resulted in good adherence to both xylene and chloroform hydrocarbons. The Bacillus spp. isolated displayed high survivability towards 0.3% bile salt and exhibited amylase, protease, and lipase activities. Thus, the isolated Bacillus spp. are considered safe based on the sensitivity analysis towards antibiotics and γ-haemolytic activity. Elsevier 2022 Article PeerReviewed Sam-on, Muhamad Firdaus Syahmi and Mustafa, Shuhaimi and Yusof, Mohd Termizi and Mohd Hashim, Amalia and Abbasiliasi, Sahar and Zulkifly, Shahrizim and Jahari, Muhamad Amin and Roslan, Muhamad Akhmal Hakim (2022) Evaluation of three Bacillus spp. isolated from the gut of giant freshwater prawn as potential probiotics against pathogens causing Vibriosis and Aeromonosis. Microbial Pathogenesis, 164. art. no. 105417. pp. 1-12. ISSN 0882-4010; ESSN: 1096-1208 https://www.sciencedirect.com/science/article/pii/S0882401022000304 10.1016/j.micpath.2022.105417 |
| spellingShingle | Sam-on, Muhamad Firdaus Syahmi Mustafa, Shuhaimi Yusof, Mohd Termizi Mohd Hashim, Amalia Abbasiliasi, Sahar Zulkifly, Shahrizim Jahari, Muhamad Amin Roslan, Muhamad Akhmal Hakim Evaluation of three Bacillus spp. isolated from the gut of giant freshwater prawn as potential probiotics against pathogens causing Vibriosis and Aeromonosis |
| title | Evaluation of three Bacillus spp. isolated from the gut of giant freshwater prawn as potential probiotics against pathogens causing Vibriosis and Aeromonosis |
| title_full | Evaluation of three Bacillus spp. isolated from the gut of giant freshwater prawn as potential probiotics against pathogens causing Vibriosis and Aeromonosis |
| title_fullStr | Evaluation of three Bacillus spp. isolated from the gut of giant freshwater prawn as potential probiotics against pathogens causing Vibriosis and Aeromonosis |
| title_full_unstemmed | Evaluation of three Bacillus spp. isolated from the gut of giant freshwater prawn as potential probiotics against pathogens causing Vibriosis and Aeromonosis |
| title_short | Evaluation of three Bacillus spp. isolated from the gut of giant freshwater prawn as potential probiotics against pathogens causing Vibriosis and Aeromonosis |
| title_sort | evaluation of three bacillus spp. isolated from the gut of giant freshwater prawn as potential probiotics against pathogens causing vibriosis and aeromonosis |
| url | http://psasir.upm.edu.my/id/eprint/101345/ http://psasir.upm.edu.my/id/eprint/101345/ http://psasir.upm.edu.my/id/eprint/101345/ |