Establishment of effective plantlets regeneration protocol via isolated microspore culture in Malaysian indica rice MR219
The current study recognised the issues encountered in regenerating Malaysia MR219 rice plantlet via microspore culture and attempted to develop an efficient protocol in overcoming the restraints. In the present study, a high proportion of uninucleate microspores (49.17%) was isolated from Stage 2-S...
| Main Authors: | , , , , , , , , , , , |
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| Format: | Article |
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Springer
2022
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| Online Access: | http://psasir.upm.edu.my/id/eprint/101301/ |
| _version_ | 1848863533037518848 |
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| author | Abd Rahman, Zuraida Ahmad Seman, Zulkifli Othman, Ayu Nazreena Ab Ghaffar, Mohamad Bahagia Ab Razak, Shahril Mohd Yusof, Muhammad Fairuz Nasir, Khairun Hisam Ahmad, Khairulmazmi Yeow, Lit Chow Teo, Chee How Md Saad, Norsharina Subramaniam, Sreeramanan |
| author_facet | Abd Rahman, Zuraida Ahmad Seman, Zulkifli Othman, Ayu Nazreena Ab Ghaffar, Mohamad Bahagia Ab Razak, Shahril Mohd Yusof, Muhammad Fairuz Nasir, Khairun Hisam Ahmad, Khairulmazmi Yeow, Lit Chow Teo, Chee How Md Saad, Norsharina Subramaniam, Sreeramanan |
| author_sort | Abd Rahman, Zuraida |
| building | UPM Institutional Repository |
| collection | Online Access |
| description | The current study recognised the issues encountered in regenerating Malaysia MR219 rice plantlet via microspore culture and attempted to develop an efficient protocol in overcoming the restraints. In the present study, a high proportion of uninucleate microspores (49.17%) was isolated from Stage 2-Segment II panicle (59–61 days), which also exhibited the highest callus initiation rate of 8.50%. Maintenance of the panicles under a cool temperature of 4 °C for 7 days before isolating the microspores, resulted in the highest microspore viability of 58.33% and callus initiation rate of 9.33%. The microspore isolation protocol was also optimised in the present study. The filtration sieve engagement with a pore size of 80 µm and further suspension centrifugation at 800 rpm for 5 min produced the highest microspore viability percentage and callus initiation rate. The incorporation of 3.0 mg/L kinetin in conjunction with 0.5 mg/L 2,4-D greatly enhanced the callus initiation rate, with 11.33%. The callus proliferation capacity, with the formation of 481.67 mg callus, was significantly promoted by the addition of 1.0 mg/L kinetin and 0.5 mg/L 2,4-D into the growth medium. Moreover, a higher green plantlet regeneration frequency of 2.83% was induced by the supplementation of 8% sucrose, which produced an average of 3.50 green plantlets. |
| first_indexed | 2025-11-15T13:34:25Z |
| format | Article |
| id | upm-101301 |
| institution | Universiti Putra Malaysia |
| institution_category | Local University |
| last_indexed | 2025-11-15T13:34:25Z |
| publishDate | 2022 |
| publisher | Springer |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | upm-1013012023-12-15T23:27:58Z http://psasir.upm.edu.my/id/eprint/101301/ Establishment of effective plantlets regeneration protocol via isolated microspore culture in Malaysian indica rice MR219 Abd Rahman, Zuraida Ahmad Seman, Zulkifli Othman, Ayu Nazreena Ab Ghaffar, Mohamad Bahagia Ab Razak, Shahril Mohd Yusof, Muhammad Fairuz Nasir, Khairun Hisam Ahmad, Khairulmazmi Yeow, Lit Chow Teo, Chee How Md Saad, Norsharina Subramaniam, Sreeramanan The current study recognised the issues encountered in regenerating Malaysia MR219 rice plantlet via microspore culture and attempted to develop an efficient protocol in overcoming the restraints. In the present study, a high proportion of uninucleate microspores (49.17%) was isolated from Stage 2-Segment II panicle (59–61 days), which also exhibited the highest callus initiation rate of 8.50%. Maintenance of the panicles under a cool temperature of 4 °C for 7 days before isolating the microspores, resulted in the highest microspore viability of 58.33% and callus initiation rate of 9.33%. The microspore isolation protocol was also optimised in the present study. The filtration sieve engagement with a pore size of 80 µm and further suspension centrifugation at 800 rpm for 5 min produced the highest microspore viability percentage and callus initiation rate. The incorporation of 3.0 mg/L kinetin in conjunction with 0.5 mg/L 2,4-D greatly enhanced the callus initiation rate, with 11.33%. The callus proliferation capacity, with the formation of 481.67 mg callus, was significantly promoted by the addition of 1.0 mg/L kinetin and 0.5 mg/L 2,4-D into the growth medium. Moreover, a higher green plantlet regeneration frequency of 2.83% was induced by the supplementation of 8% sucrose, which produced an average of 3.50 green plantlets. Springer 2022-01-20 Article PeerReviewed Abd Rahman, Zuraida and Ahmad Seman, Zulkifli and Othman, Ayu Nazreena and Ab Ghaffar, Mohamad Bahagia and Ab Razak, Shahril and Mohd Yusof, Muhammad Fairuz and Nasir, Khairun Hisam and Ahmad, Khairulmazmi and Yeow, Lit Chow and Teo, Chee How and Md Saad, Norsharina and Subramaniam, Sreeramanan (2022) Establishment of effective plantlets regeneration protocol via isolated microspore culture in Malaysian indica rice MR219. Plant Biotechnology Reports, 16 (3). pp. 343-355. ISSN 1863-5466; ESSN: 1863-5474 https://link.springer.com/article/10.1007/s11816-022-00742-4 10.1007/s11816-022-00742-4 |
| spellingShingle | Abd Rahman, Zuraida Ahmad Seman, Zulkifli Othman, Ayu Nazreena Ab Ghaffar, Mohamad Bahagia Ab Razak, Shahril Mohd Yusof, Muhammad Fairuz Nasir, Khairun Hisam Ahmad, Khairulmazmi Yeow, Lit Chow Teo, Chee How Md Saad, Norsharina Subramaniam, Sreeramanan Establishment of effective plantlets regeneration protocol via isolated microspore culture in Malaysian indica rice MR219 |
| title | Establishment of effective plantlets regeneration protocol via isolated microspore culture in Malaysian indica rice MR219 |
| title_full | Establishment of effective plantlets regeneration protocol via isolated microspore culture in Malaysian indica rice MR219 |
| title_fullStr | Establishment of effective plantlets regeneration protocol via isolated microspore culture in Malaysian indica rice MR219 |
| title_full_unstemmed | Establishment of effective plantlets regeneration protocol via isolated microspore culture in Malaysian indica rice MR219 |
| title_short | Establishment of effective plantlets regeneration protocol via isolated microspore culture in Malaysian indica rice MR219 |
| title_sort | establishment of effective plantlets regeneration protocol via isolated microspore culture in malaysian indica rice mr219 |
| url | http://psasir.upm.edu.my/id/eprint/101301/ http://psasir.upm.edu.my/id/eprint/101301/ http://psasir.upm.edu.my/id/eprint/101301/ |