Effect of an oral bivalent vaccine on immune response and immune gene profiling in vaccinated red tilapia (Oreochromis spp.) during infections with Streptococcus iniae and Aeromonas hydrophila
Streptococcosis and aeromonasis inflicted by Streptococcus iniae and Aeromonas hydrophila, respectively, have affected tilapia industries worldwide. In this study, we investigated antibody responses and explored the mechanisms of protection rendered by an oral bivalent vaccine in red tilapia followi...
| Main Authors: | , , , , , , |
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| Format: | Article |
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MDPI
2022
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| Online Access: | http://psasir.upm.edu.my/id/eprint/101078/ |
| _version_ | 1848863488138543104 |
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| author | Monir, Md Shirajum Mohd Yusoff, Md Sabri Saad, Mohd Zamri Amal, Mohammad Noor Azmai Mohamad, Aslah Sayuti, Mohamad Azzam Md Yasin, Ina-Salwany |
| author_facet | Monir, Md Shirajum Mohd Yusoff, Md Sabri Saad, Mohd Zamri Amal, Mohammad Noor Azmai Mohamad, Aslah Sayuti, Mohamad Azzam Md Yasin, Ina-Salwany |
| author_sort | Monir, Md Shirajum |
| building | UPM Institutional Repository |
| collection | Online Access |
| description | Streptococcosis and aeromonasis inflicted by Streptococcus iniae and Aeromonas hydrophila, respectively, have affected tilapia industries worldwide. In this study, we investigated antibody responses and explored the mechanisms of protection rendered by an oral bivalent vaccine in red tilapia following challenges with S. iniae and A. hydrophila. The results of specific IgM antibody response revealed that the IgM titers against S. iniae and A. hydrophila in the bivalent incorporated (BI) vaccine group were significantly higher (p < 0.05) than those in the bivalent spray (BS) vaccine fish and unvaccinated control fish throughout the experiment. Real-time qPCR results also showed that the gene expression of CD4, MHC-I, MHC-II, IgT, C-type lysozyme, IL-1β, TNF-α, and TGF-β remained significantly higher (p < 0.05) than that of the controls between 24 and 72 h post-infection (hpi) in both mucosal (hindgut) and systemic (spleen and head–kidney) organs of BI vaccinated fish. Furthermore, the highest relative expression of the TGF-β, C-type lysozyme, and IgT genes in the BI vaccinated group was observed in the challenged fish’s spleen (8.8-fold), head kidney (4.4-fold), and hindgut (19.7-fold) tissues, respectively. The present study suggests that the bivalent incorporated (BI) vaccine could effectively improve the immune function and activate both humoral and cell-mediated immunities in vaccinated red tilapia following the bacterial challenges. |
| first_indexed | 2025-11-15T13:33:43Z |
| format | Article |
| id | upm-101078 |
| institution | Universiti Putra Malaysia |
| institution_category | Local University |
| last_indexed | 2025-11-15T13:33:43Z |
| publishDate | 2022 |
| publisher | MDPI |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | upm-1010782023-05-24T04:10:08Z http://psasir.upm.edu.my/id/eprint/101078/ Effect of an oral bivalent vaccine on immune response and immune gene profiling in vaccinated red tilapia (Oreochromis spp.) during infections with Streptococcus iniae and Aeromonas hydrophila Monir, Md Shirajum Mohd Yusoff, Md Sabri Saad, Mohd Zamri Amal, Mohammad Noor Azmai Mohamad, Aslah Sayuti, Mohamad Azzam Md Yasin, Ina-Salwany Streptococcosis and aeromonasis inflicted by Streptococcus iniae and Aeromonas hydrophila, respectively, have affected tilapia industries worldwide. In this study, we investigated antibody responses and explored the mechanisms of protection rendered by an oral bivalent vaccine in red tilapia following challenges with S. iniae and A. hydrophila. The results of specific IgM antibody response revealed that the IgM titers against S. iniae and A. hydrophila in the bivalent incorporated (BI) vaccine group were significantly higher (p < 0.05) than those in the bivalent spray (BS) vaccine fish and unvaccinated control fish throughout the experiment. Real-time qPCR results also showed that the gene expression of CD4, MHC-I, MHC-II, IgT, C-type lysozyme, IL-1β, TNF-α, and TGF-β remained significantly higher (p < 0.05) than that of the controls between 24 and 72 h post-infection (hpi) in both mucosal (hindgut) and systemic (spleen and head–kidney) organs of BI vaccinated fish. Furthermore, the highest relative expression of the TGF-β, C-type lysozyme, and IgT genes in the BI vaccinated group was observed in the challenged fish’s spleen (8.8-fold), head kidney (4.4-fold), and hindgut (19.7-fold) tissues, respectively. The present study suggests that the bivalent incorporated (BI) vaccine could effectively improve the immune function and activate both humoral and cell-mediated immunities in vaccinated red tilapia following the bacterial challenges. MDPI 2022-08-26 Article PeerReviewed Monir, Md Shirajum and Mohd Yusoff, Md Sabri and Saad, Mohd Zamri and Amal, Mohammad Noor Azmai and Mohamad, Aslah and Sayuti, Mohamad Azzam and Md Yasin, Ina-Salwany (2022) Effect of an oral bivalent vaccine on immune response and immune gene profiling in vaccinated red tilapia (Oreochromis spp.) during infections with Streptococcus iniae and Aeromonas hydrophila. Biology, 11 (9). art. no. 1628. pp. 1-21. ISSN 2079-7737 https://www.mdpi.com/2079-7737/11/9/1268 10.3390/biology11091268 |
| spellingShingle | Monir, Md Shirajum Mohd Yusoff, Md Sabri Saad, Mohd Zamri Amal, Mohammad Noor Azmai Mohamad, Aslah Sayuti, Mohamad Azzam Md Yasin, Ina-Salwany Effect of an oral bivalent vaccine on immune response and immune gene profiling in vaccinated red tilapia (Oreochromis spp.) during infections with Streptococcus iniae and Aeromonas hydrophila |
| title | Effect of an oral bivalent vaccine on immune response and immune gene profiling in vaccinated red tilapia (Oreochromis spp.) during infections with Streptococcus iniae and Aeromonas hydrophila |
| title_full | Effect of an oral bivalent vaccine on immune response and immune gene profiling in vaccinated red tilapia (Oreochromis spp.) during infections with Streptococcus iniae and Aeromonas hydrophila |
| title_fullStr | Effect of an oral bivalent vaccine on immune response and immune gene profiling in vaccinated red tilapia (Oreochromis spp.) during infections with Streptococcus iniae and Aeromonas hydrophila |
| title_full_unstemmed | Effect of an oral bivalent vaccine on immune response and immune gene profiling in vaccinated red tilapia (Oreochromis spp.) during infections with Streptococcus iniae and Aeromonas hydrophila |
| title_short | Effect of an oral bivalent vaccine on immune response and immune gene profiling in vaccinated red tilapia (Oreochromis spp.) during infections with Streptococcus iniae and Aeromonas hydrophila |
| title_sort | effect of an oral bivalent vaccine on immune response and immune gene profiling in vaccinated red tilapia (oreochromis spp.) during infections with streptococcus iniae and aeromonas hydrophila |
| url | http://psasir.upm.edu.my/id/eprint/101078/ http://psasir.upm.edu.my/id/eprint/101078/ http://psasir.upm.edu.my/id/eprint/101078/ |