DNA free CRISPR/DCAS9 based transcriptional activation system for UGT76G1 gene in Stevia rebaudiana Bertoni protoplasts

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DNA free CRISPR/DCAS9 based transcriptional activation system for UGT76G1 gene in Stevia rebaudiana Bertoni protoplasts

The UDP-glycosyltransferase 76G1 (UGT76G1) is responsible for the conversion of stevioside to rebaudioside A. Four single guide RNAs (sgRNAs) were designed from the UGT76G1 proximal promoter region of stevia by using the online-based tool, benchling. The dCas9 fused with VP64 as a transcriptional ac...

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Bibliographic Details
Main Authors:	Ghose, Asish Kumar, Abdullah, Siti Nor Akmar, Md Hatta, Muhammad Asyraf, Megat Wahab, Puteri Edaroyati
Format:	Article
Published:	MDPI 2022
Online Access:	http://psasir.upm.edu.my/id/eprint/100989/

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