Designing gRNAs targeting oil palm phytoene desaturase (EgPDS) gene and construction of vectors for oil palm CRISPR/Cas9 study

QR Code

Designing gRNAs targeting oil palm phytoene desaturase (EgPDS) gene and construction of vectors for oil palm CRISPR/Cas9 study

The CRISPR/Cas9 system is a precise and versatile genetic tool used to induce site-specific manipulation in the targeted gene. This study elaborates on the strategy to select the gRNAs targeting the oil palm phytoene desaturase (EgPDS) gene, which complements CRISPR-GE and sequence analysis. Sixty-o...

Full description

Bibliographic Details
Main Authors:	Jamaludin, Norfaezah, Bahariah, Bohari, Shaharuddin, Noor Azmi, Chai, Ling Ho, Abdul Rasid, Omar, Ahmad Parveez, Ghulam Kadir, Abdul Masani, Mat Yunus
Format:	Article
Published:	Malaysian Palm Oil Board 2022
Online Access:	http://psasir.upm.edu.my/id/eprint/100905/

Similar Items

Gene editing the phytoene desaturase alleles of Cavendish banana using CRISPR/Cas9
by: Eftekhari, Fatima, et al.
Published: (2018)

Towards DNA‑free CRISPR/Cas9 genome editing for sustainable oil palm improvement
by: Shaharuddin, Noor Azmi, et al.
Published: (2024)

DNA-free CRISPR/Cas9 genome editing system for oil palm protoplasts using multiple ribonucleoproteins (RNPs) complexes
by: Norfaezah, Jamaludin, et al.
Published: (2024)

EgRBP42 from oil palm enhances adaptation to stress tolerance in Arabidopsis through regulation of nucleocytoplasmic transport of stress-responsive mRNAs
by: Yeap, Wan Chin, et al.
Published: (2019)

RT-PCR amplification and cloning of partial DNA sequence coding for oil palm (Elaeis oleifera) phytoene synthase gene
by: Abd Rasid, Omar, et al.
Published: (2008)

The CRISPR growth spurt: from bench to clinic on versatile small RNAs
by: Bayat, Hadi, et al.
Published: (2017)

Cloning and Characterization of Oleoyl-Coa Desaturase Gene from Oil Palm (Elaeis Guineensis L.)
by: Shahwan, Syahanim
Published: (2006)

Construction of PHB and PHBV transformation vectors for bioplastics production in oil palm
by: Mat Yunus, Abdul Masani, et al.
Published: (2008)

Overexpression of oil palm EgDREB1 in tomato decreased fruit size and produced parthenocarpic fruits
by: Mohamad Azzeme, Azzreena, et al.
Published: (2020)

Identification of regulatory motif for enhancing expression of oil palm (Elaeis guineensis Jacq.) stearoyl-ACP desaturase 1
by: Abu Hanifiah, Farah Hanan
Published: (2018)

Egg-shell treated oil palm fronds (EG-OPF) as low-cost adsorbent for methylene blue removal
by: Rosalyza, Hasan, et al.
Published: (2019)

Isolation and characterization of EgGST, a glutathione S-transferase protein transcript in oil palm (Elaeis guineensis Jacq.)
by: Conie, Toh, et al.
Published: (2015)

Isolation and characterization of an ethylene-responsive element binding protein (EgEREBP) from oil palm (Elaeis guineensis)
by: Omidvar, Vahid, et al.
Published: (2013)

The role of Cas8 in type I CRISPR interference
by: Cass, Simon, et al.
Published: (2015)

Cloning and analysis of the Eg4CL1 gene and its promoter from oil palm (Elaeis guineensis Jacq.)
by: Chong, Yusuf Yu Lok, et al.
Published: (2018)

A novel transcript of oil palm (Elaeis guineensis Jacq.), Eg707, is specifically upregulated in tissues related to totipotency
by: Thuc, Le Vinh, et al.
Published: (2011)

Efficient PEG-mediated transformation of oil palm mesophyll protoplasts and its application in functional analysis of oil palm promoters
by: Fizree, MD Piji Mohd Al Akmarul, et al.
Published: (2023)

Oil palm EgCBF3 conferred stress tolerance in transgenic tomato plants through modulation of the ethylene signaling pathway
by: Ebrahimi, Mortaza, et al.
Published: (2016)

Investigation of CRISPR-Cas adaptation mechanism through exploration of Cas4-1 fusions
by: Walker, Emily
Published: (2019)

CRISPR-Cas immunity: analysis of adaptation and interference reactions in prokaryotes
by: Cass, S.D.B.
Published: (2016)

GCTTCA as a novel motif for regulating mesocarp-specific expression of the oil palm (Elaeis guineensis Jacq.) stearoyl-ACP desaturase gene
by: Abu Hanifiah, Farah Hanan, et al.
Published: (2018)

Sequence and expression analysis of EgSAPK, a putative member of the serine/threonine protein kinases in oil palm (Elaeis guineensis Jacq.)
by: Huynh, Ky, et al.
Published: (2009)

Methylation levels of a novel genetic element, EgNB3 as a candidate biomarker associated with the embryogenic competency of oil palm
by: Ho, W. K., et al.
Published: (2013)

Gene expression of the oil palm transcription factor EgAP2-1 during fruit ripening and in response to ethylene and ABA treatments.
by: Omidvar, Vahid, et al.
Published: (2013)

Identification of natural host range of coconut cadang-cadang viroid and characterization of its small RNAs from oil palm
by: Mohammadi, Mohammadreza
Published: (2014)

Gene expression and promoter characterisation of stearoyl-ACP desaturase and acyl carrier protein genes from oil palm (Elaeis guineensis jacq.) fruits
by: Othman, Ashida
Published: (2010)

The oil palm stearoyl-acyl-carrier-protein desaturase (Des) promoter drives transient gene expression in tomato fruits and is affected by gibberellic acid
by: Leong, Siow Li, et al.
Published: (2013)

Optimization of Parameters Involved in the Transformation of Oil Palm Using the Biolistic Method
by: Ghulam Kadir, Ahmad Parveez
Published: (1998)

Characterising Host Factors Required for CRISPR Cas Adaptation in Escherichia coli
by: Edwards, Harry
Published: (2023)

Harnessing CRISPR-Cas to combat COVID-19: from diagnostics to therapeutics
by: Kok, Gan Chan, et al.
Published: (2021)

CRISPR-Cas knockout of miR21 reduces glioma growth
by: Nieland, Lisa, et al.
Published: (2022)

The enhancement of plant disease resistance using CRISPR/Cas9 technology
by: Mohd Azman, Amier Asyraf, et al.
Published: (2022)

Production of Specialized Transformation Vectors for the Production of Biodegradable Plastics in Transgenic Arabidopsis and Oil Palm
by: Mat Yunus, Abdul Masani
Published: (2006)

Analysis of Cas1-2 and Cas3 CRISPR immunity proteins in E. coli cell physiology
by: Reeves, Ryan Peter
Published: (2019)

Molecular insights into DNA interference by CRISPR-associated nuclease-helicase Cas3
by: Gong, Bei, et al.
Published: (2014)

CRISPR systems - molecular mechanisms of the interaction between Cas1 and DnaK
by: Stewart, Alison L.
Published: (2025)

Applications of a high-throughput screen detecting CRISPR-Cas spacer acquisition
by: Cannon, Christopher
Published: (2025)

Combinatorial Crispr/Cas9 For Suppression Of Latent Hiv-1 Provirus In Vitro
by: Ravichantar, Nithya
Published: (2020)

Overexpression of the oil palm (Elaeis guineensis Jacq.) TAPETUM DEVELOPMENT1-like Eg707 in rice affects cell division and differentiation and reduces fertility
by: Thuc, Le Vinh, et al.
Published: (2013)

Construction of PHB and PHBV multiple-gene vectors driven by an oil palm leaf-specific promoter
by: Mat Yunus, Abdul Masani, et al.
Published: (2009)