Comprehensive approaches for the detection of Burkholderia pseudomallei and diagnosis of melioidosis in human and environmental samples
Melioidosis is endemic in Southeast Asia and northern Australia. The causative agent of melioidosis is a Gram-negative bacterium, Burkholderia pseudomallei. Its invasion can be fatal if melioidosis is not treated promptly. It is intrinsically resistant to a variety of antibiotics. In this paper, we...
| Main Authors: | , , , , , , |
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| Format: | Article |
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Elsevier
2022
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| Online Access: | http://psasir.upm.edu.my/id/eprint/100766/ |
| _version_ | 1848863408825303040 |
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| author | Oslan, Siti Nur Hazwani Yusoff, Abdul Hafidz Mazlan, Mazlina Si, Jie Lim Jing, Jing Khoo Oslan, Siti Nurbaya Ismail, Aziah |
| author_facet | Oslan, Siti Nur Hazwani Yusoff, Abdul Hafidz Mazlan, Mazlina Si, Jie Lim Jing, Jing Khoo Oslan, Siti Nurbaya Ismail, Aziah |
| author_sort | Oslan, Siti Nur Hazwani |
| building | UPM Institutional Repository |
| collection | Online Access |
| description | Melioidosis is endemic in Southeast Asia and northern Australia. The causative agent of melioidosis is a Gram-negative bacterium, Burkholderia pseudomallei. Its invasion can be fatal if melioidosis is not treated promptly. It is intrinsically resistant to a variety of antibiotics. In this paper, we present a comprehensive overview of the current trends on melioidosis cases, treatments, B. pseudomallei virulence factors, and molecular techniques to detect the bacterium from different samples. The clinical and microbial diagnosis methods of identification and detection of B. pseudomallei are commonly used for the rapid diagnosis and typing of strains, such as polymerase chain reaction or multi-locus sequence typing. The genotyping strategies and techniques have been constantly evolving to identify genomic loci linked to or associated with this human disease. More research strategies for detecting and controlling melioidosis should be encouraged and conducted to understand the current situation. In conclusion, we review existing diagnostic methodologies for melioidosis detection and provide insights on prospective diagnostic methods for the bacterium. |
| first_indexed | 2025-11-15T13:32:27Z |
| format | Article |
| id | upm-100766 |
| institution | Universiti Putra Malaysia |
| institution_category | Local University |
| last_indexed | 2025-11-15T13:32:27Z |
| publishDate | 2022 |
| publisher | Elsevier |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | upm-1007662023-09-11T01:35:02Z http://psasir.upm.edu.my/id/eprint/100766/ Comprehensive approaches for the detection of Burkholderia pseudomallei and diagnosis of melioidosis in human and environmental samples Oslan, Siti Nur Hazwani Yusoff, Abdul Hafidz Mazlan, Mazlina Si, Jie Lim Jing, Jing Khoo Oslan, Siti Nurbaya Ismail, Aziah Melioidosis is endemic in Southeast Asia and northern Australia. The causative agent of melioidosis is a Gram-negative bacterium, Burkholderia pseudomallei. Its invasion can be fatal if melioidosis is not treated promptly. It is intrinsically resistant to a variety of antibiotics. In this paper, we present a comprehensive overview of the current trends on melioidosis cases, treatments, B. pseudomallei virulence factors, and molecular techniques to detect the bacterium from different samples. The clinical and microbial diagnosis methods of identification and detection of B. pseudomallei are commonly used for the rapid diagnosis and typing of strains, such as polymerase chain reaction or multi-locus sequence typing. The genotyping strategies and techniques have been constantly evolving to identify genomic loci linked to or associated with this human disease. More research strategies for detecting and controlling melioidosis should be encouraged and conducted to understand the current situation. In conclusion, we review existing diagnostic methodologies for melioidosis detection and provide insights on prospective diagnostic methods for the bacterium. Elsevier 2022-08 Article PeerReviewed Oslan, Siti Nur Hazwani and Yusoff, Abdul Hafidz and Mazlan, Mazlina and Si, Jie Lim and Jing, Jing Khoo and Oslan, Siti Nurbaya and Ismail, Aziah (2022) Comprehensive approaches for the detection of Burkholderia pseudomallei and diagnosis of melioidosis in human and environmental samples. Microbial Pathogenesis, 169 (105637). art. no. https://www.sciencedirect.com/science/article/pii/S0882401022002509. pp. 1-10. ISSN 0882-4010 https://www.sciencedirect.com/science/article/pii/S0882401022002509 10.1016/j.micpath.2022.105637 |
| spellingShingle | Oslan, Siti Nur Hazwani Yusoff, Abdul Hafidz Mazlan, Mazlina Si, Jie Lim Jing, Jing Khoo Oslan, Siti Nurbaya Ismail, Aziah Comprehensive approaches for the detection of Burkholderia pseudomallei and diagnosis of melioidosis in human and environmental samples |
| title | Comprehensive approaches for the detection of Burkholderia pseudomallei and diagnosis of melioidosis in human and environmental samples |
| title_full | Comprehensive approaches for the detection of Burkholderia pseudomallei and diagnosis of melioidosis in human and environmental samples |
| title_fullStr | Comprehensive approaches for the detection of Burkholderia pseudomallei and diagnosis of melioidosis in human and environmental samples |
| title_full_unstemmed | Comprehensive approaches for the detection of Burkholderia pseudomallei and diagnosis of melioidosis in human and environmental samples |
| title_short | Comprehensive approaches for the detection of Burkholderia pseudomallei and diagnosis of melioidosis in human and environmental samples |
| title_sort | comprehensive approaches for the detection of burkholderia pseudomallei and diagnosis of melioidosis in human and environmental samples |
| url | http://psasir.upm.edu.my/id/eprint/100766/ http://psasir.upm.edu.my/id/eprint/100766/ http://psasir.upm.edu.my/id/eprint/100766/ |