Characterization of carboxylic acid reductase from Mycobacterium phlei immobilized onto Seplite LX120
A multi-domain oxidoreductase, carboxylic acid reductase (CAR), can catalyze the one-step reduction of carboxylic acid to aldehyde. This study aimed to immobilize bacterial CAR from a moderate thermophile Mycobacterium phlei (MpCAR). It was the first work reported on immobilizing bacterial CAR onto...
| Main Authors: | , , , , |
|---|---|
| Format: | Article |
| Published: |
MDPI
2022
|
| Online Access: | http://psasir.upm.edu.my/id/eprint/100675/ |
| _version_ | 1848863385962151936 |
|---|---|
| author | Basri, Rose Syuhada Raja Abd. Rahman, Raja Noor Zaliha Ahmad Kamarudin, Nor Hafizah Latip, Wahhida Mohamad Ali, Mohd Shukuri |
| author_facet | Basri, Rose Syuhada Raja Abd. Rahman, Raja Noor Zaliha Ahmad Kamarudin, Nor Hafizah Latip, Wahhida Mohamad Ali, Mohd Shukuri |
| author_sort | Basri, Rose Syuhada |
| building | UPM Institutional Repository |
| collection | Online Access |
| description | A multi-domain oxidoreductase, carboxylic acid reductase (CAR), can catalyze the one-step reduction of carboxylic acid to aldehyde. This study aimed to immobilize bacterial CAR from a moderate thermophile Mycobacterium phlei (MpCAR). It was the first work reported on immobilizing bacterial CAR onto a polymeric support, Seplite LX120, via simple adsorption. Immobilization time and protein load were optimized for MpCAR immobilization. The immobilized MpCAR showed optimal activity at 60 °C and pH 9. It was stable over a wide range of temperatures (10 to 100 °C) and pHs (4–11), retaining more than 50% of its activity. The immobilized MpCAR also showed stability in polar solvents. The adsorption of MpCAR onto the support was confirmed by Scanning Electron Microscopy (SEM), Fourier-Transform Infrared (FTIR) spectroscopy, and Brunauer–Emmett–Teller (BET) analysis. The immobilized MpCAR could be stored for up to 6 weeks at 4 °C and 3 weeks at 25 °C. Immobilized MpCAR showed great operational stability, as 59.68% of its activity was preserved after 10 assay cycles. The immobilized MpCAR could also convert approximately 2.6 mM of benzoic acid to benzaldehyde at 60 °C. The successfully immobilized MpCAR on Seplite LX120 exhibited improved properties that benefit green industrial processes. |
| first_indexed | 2025-11-15T13:32:05Z |
| format | Article |
| id | upm-100675 |
| institution | Universiti Putra Malaysia |
| institution_category | Local University |
| last_indexed | 2025-11-15T13:32:05Z |
| publishDate | 2022 |
| publisher | MDPI |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | upm-1006752023-09-21T04:35:10Z http://psasir.upm.edu.my/id/eprint/100675/ Characterization of carboxylic acid reductase from Mycobacterium phlei immobilized onto Seplite LX120 Basri, Rose Syuhada Raja Abd. Rahman, Raja Noor Zaliha Ahmad Kamarudin, Nor Hafizah Latip, Wahhida Mohamad Ali, Mohd Shukuri A multi-domain oxidoreductase, carboxylic acid reductase (CAR), can catalyze the one-step reduction of carboxylic acid to aldehyde. This study aimed to immobilize bacterial CAR from a moderate thermophile Mycobacterium phlei (MpCAR). It was the first work reported on immobilizing bacterial CAR onto a polymeric support, Seplite LX120, via simple adsorption. Immobilization time and protein load were optimized for MpCAR immobilization. The immobilized MpCAR showed optimal activity at 60 °C and pH 9. It was stable over a wide range of temperatures (10 to 100 °C) and pHs (4–11), retaining more than 50% of its activity. The immobilized MpCAR also showed stability in polar solvents. The adsorption of MpCAR onto the support was confirmed by Scanning Electron Microscopy (SEM), Fourier-Transform Infrared (FTIR) spectroscopy, and Brunauer–Emmett–Teller (BET) analysis. The immobilized MpCAR could be stored for up to 6 weeks at 4 °C and 3 weeks at 25 °C. Immobilized MpCAR showed great operational stability, as 59.68% of its activity was preserved after 10 assay cycles. The immobilized MpCAR could also convert approximately 2.6 mM of benzoic acid to benzaldehyde at 60 °C. The successfully immobilized MpCAR on Seplite LX120 exhibited improved properties that benefit green industrial processes. MDPI 2022-10-17 Article PeerReviewed Basri, Rose Syuhada and Raja Abd. Rahman, Raja Noor Zaliha and Ahmad Kamarudin, Nor Hafizah and Latip, Wahhida and Mohamad Ali, Mohd Shukuri (2022) Characterization of carboxylic acid reductase from Mycobacterium phlei immobilized onto Seplite LX120. Polymers, 14 (20). art. no. 4375. pp. 1-19. ISSN 2073-4360 https://www.mdpi.com/2073-4360/14/20/4375 10.3390/polym14204375 |
| spellingShingle | Basri, Rose Syuhada Raja Abd. Rahman, Raja Noor Zaliha Ahmad Kamarudin, Nor Hafizah Latip, Wahhida Mohamad Ali, Mohd Shukuri Characterization of carboxylic acid reductase from Mycobacterium phlei immobilized onto Seplite LX120 |
| title | Characterization of carboxylic acid reductase from Mycobacterium phlei immobilized onto Seplite LX120 |
| title_full | Characterization of carboxylic acid reductase from Mycobacterium phlei immobilized onto Seplite LX120 |
| title_fullStr | Characterization of carboxylic acid reductase from Mycobacterium phlei immobilized onto Seplite LX120 |
| title_full_unstemmed | Characterization of carboxylic acid reductase from Mycobacterium phlei immobilized onto Seplite LX120 |
| title_short | Characterization of carboxylic acid reductase from Mycobacterium phlei immobilized onto Seplite LX120 |
| title_sort | characterization of carboxylic acid reductase from mycobacterium phlei immobilized onto seplite lx120 |
| url | http://psasir.upm.edu.my/id/eprint/100675/ http://psasir.upm.edu.my/id/eprint/100675/ http://psasir.upm.edu.my/id/eprint/100675/ |