Characterisation and selection of freshwater cyanobacteria for phycobiliprotein contents
Some cyanobacteria species have a high capacity for accumulating phycobiliprotein contents in their cells. However, there is a lack of information on screening tropical freshwater cyanobacteria, particularly phycobiliproteins. In addition, it is unclear which characteristics of cyanobacteria (morpho...
| Main Authors: | , , , , , , , |
|---|---|
| Format: | Article |
| Published: |
Springer Nature
2022
|
| Online Access: | http://psasir.upm.edu.my/id/eprint/100661/ |
| _version_ | 1848863382133800960 |
|---|---|
| author | Hui, Teng Tan Md. Yusoff, Fatimah Yam, Sim Khaw Nazarudin, Muhammad Farhan Noor Mazli, Nur Amirah Izyan Ahmad, Siti Aqlima Shaharuddin, Noor Azmi Toda, Tatsuki |
| author_facet | Hui, Teng Tan Md. Yusoff, Fatimah Yam, Sim Khaw Nazarudin, Muhammad Farhan Noor Mazli, Nur Amirah Izyan Ahmad, Siti Aqlima Shaharuddin, Noor Azmi Toda, Tatsuki |
| author_sort | Hui, Teng Tan |
| building | UPM Institutional Repository |
| collection | Online Access |
| description | Some cyanobacteria species have a high capacity for accumulating phycobiliprotein contents in their cells. However, there is a lack of information on screening tropical freshwater cyanobacteria, particularly phycobiliproteins. In addition, it is unclear which characteristics of cyanobacteria (morphological and/or growth) could affect phycobiliprotein contents. This study aimed to screen and characterise Malaysian indigenous freshwater cyanobacteria for the growth, biomass, and pigment contents and determine the major characteristic that contributed to the variation of phycobiliproteins. The surface/volume (S/V) ratio, specific growth rate, biomass productivity, and pigment contents of the isolated cyanobacteria were analysed. Principal component analysis (PCA) and hierarchical cluster analysis (HCA) were applied to distinguish the factor responsible for phycobiliprotein variations in cyanobacteria. For the phycobiliprotein contents, Arthrospira sp., Pseudanabaena sp., and Synechococcus elongatus showed significantly higher (p < 0.05) amounts of cyanobacterial phycocyanin (C-PC), phycoerythrin (C-PE), and allophycocyanin (C-APC), respectively, than other studied cyanobacteria. This study showed no apparent trend of similarity and difference between the unicellular and filamentous cyanobacteria. In addition, carotenoid contents demonstrated a positive correlation with total phycobiliproteins, C-PC and C-APC. Based on the current findings, Arthrospira sp., Pseudanabaena sp. and Synechococcus elongatus might be the promising candidate to be the C-PC, C-PE and C-APC sources, respectively, for commercial production purposes. The selection of optimal cyanobacterial strain is crucial for efficient phycobiliprotein production. This study underlined the potential of freshwater cyanobacteria in producing respective and total phycobiliproteins. Future studies such as the optimization process should be adopted to improve the phycobiliprotein production of these cyanobacteria significantly. |
| first_indexed | 2025-11-15T13:32:02Z |
| format | Article |
| id | upm-100661 |
| institution | Universiti Putra Malaysia |
| institution_category | Local University |
| last_indexed | 2025-11-15T13:32:02Z |
| publishDate | 2022 |
| publisher | Springer Nature |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | upm-1006612023-09-15T08:17:55Z http://psasir.upm.edu.my/id/eprint/100661/ Characterisation and selection of freshwater cyanobacteria for phycobiliprotein contents Hui, Teng Tan Md. Yusoff, Fatimah Yam, Sim Khaw Nazarudin, Muhammad Farhan Noor Mazli, Nur Amirah Izyan Ahmad, Siti Aqlima Shaharuddin, Noor Azmi Toda, Tatsuki Some cyanobacteria species have a high capacity for accumulating phycobiliprotein contents in their cells. However, there is a lack of information on screening tropical freshwater cyanobacteria, particularly phycobiliproteins. In addition, it is unclear which characteristics of cyanobacteria (morphological and/or growth) could affect phycobiliprotein contents. This study aimed to screen and characterise Malaysian indigenous freshwater cyanobacteria for the growth, biomass, and pigment contents and determine the major characteristic that contributed to the variation of phycobiliproteins. The surface/volume (S/V) ratio, specific growth rate, biomass productivity, and pigment contents of the isolated cyanobacteria were analysed. Principal component analysis (PCA) and hierarchical cluster analysis (HCA) were applied to distinguish the factor responsible for phycobiliprotein variations in cyanobacteria. For the phycobiliprotein contents, Arthrospira sp., Pseudanabaena sp., and Synechococcus elongatus showed significantly higher (p < 0.05) amounts of cyanobacterial phycocyanin (C-PC), phycoerythrin (C-PE), and allophycocyanin (C-APC), respectively, than other studied cyanobacteria. This study showed no apparent trend of similarity and difference between the unicellular and filamentous cyanobacteria. In addition, carotenoid contents demonstrated a positive correlation with total phycobiliproteins, C-PC and C-APC. Based on the current findings, Arthrospira sp., Pseudanabaena sp. and Synechococcus elongatus might be the promising candidate to be the C-PC, C-PE and C-APC sources, respectively, for commercial production purposes. The selection of optimal cyanobacterial strain is crucial for efficient phycobiliprotein production. This study underlined the potential of freshwater cyanobacteria in producing respective and total phycobiliproteins. Future studies such as the optimization process should be adopted to improve the phycobiliprotein production of these cyanobacteria significantly. Springer Nature 2022-10-03 Article PeerReviewed Hui, Teng Tan and Md. Yusoff, Fatimah and Yam, Sim Khaw and Nazarudin, Muhammad Farhan and Noor Mazli, Nur Amirah Izyan and Ahmad, Siti Aqlima and Shaharuddin, Noor Azmi and Toda, Tatsuki (2022) Characterisation and selection of freshwater cyanobacteria for phycobiliprotein contents. Aquaculture International, 31 (1). pp. 447-477. ISSN 0967-6120; ESSN: 1573-143X https://link.springer.com/article/10.1007/s10499-022-00985-6 10.1007/s10499-022-00985-6 |
| spellingShingle | Hui, Teng Tan Md. Yusoff, Fatimah Yam, Sim Khaw Nazarudin, Muhammad Farhan Noor Mazli, Nur Amirah Izyan Ahmad, Siti Aqlima Shaharuddin, Noor Azmi Toda, Tatsuki Characterisation and selection of freshwater cyanobacteria for phycobiliprotein contents |
| title | Characterisation and selection of freshwater cyanobacteria for phycobiliprotein contents |
| title_full | Characterisation and selection of freshwater cyanobacteria for phycobiliprotein contents |
| title_fullStr | Characterisation and selection of freshwater cyanobacteria for phycobiliprotein contents |
| title_full_unstemmed | Characterisation and selection of freshwater cyanobacteria for phycobiliprotein contents |
| title_short | Characterisation and selection of freshwater cyanobacteria for phycobiliprotein contents |
| title_sort | characterisation and selection of freshwater cyanobacteria for phycobiliprotein contents |
| url | http://psasir.upm.edu.my/id/eprint/100661/ http://psasir.upm.edu.my/id/eprint/100661/ http://psasir.upm.edu.my/id/eprint/100661/ |