Centella asiatica L. Urban protects against morphological aberrations induced by chronic unpredictable mild stress in rat’s hippocampus via attenuation of oxidative stress

The present study evaluated the effect of Centella asiatica L. Urban (CA) extracts on ultrastructure and oxidative stress parameters on chronic unpredictable mild stress (CUMS) rat’s hippocampi. Rats were divided into six groups (n=6) and treated as follows: Group 1, Unstressed + normal saline (cont...

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Main Authors: Jagadeesan, Saravanan, Chiroma, Samaila Musa, Mohd Moklas, Mohamad Aris, Baharuldin, Mohamad Taufik Hidayat, Mat Taib, Che Norma, Amom, Zulkhairi, Vishnumukkala, Thirupathirao, Thomas, Warren, Mahdi, Onesimus
Format: Article
Published: Taylor and Francis Group 2022
Online Access:http://psasir.upm.edu.my/id/eprint/100644/
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author Jagadeesan, Saravanan
Chiroma, Samaila Musa
Mohd Moklas, Mohamad Aris
Baharuldin, Mohamad Taufik Hidayat
Mat Taib, Che Norma
Amom, Zulkhairi
Vishnumukkala, Thirupathirao
Thomas, Warren
Mahdi, Onesimus
author_facet Jagadeesan, Saravanan
Chiroma, Samaila Musa
Mohd Moklas, Mohamad Aris
Baharuldin, Mohamad Taufik Hidayat
Mat Taib, Che Norma
Amom, Zulkhairi
Vishnumukkala, Thirupathirao
Thomas, Warren
Mahdi, Onesimus
author_sort Jagadeesan, Saravanan
building UPM Institutional Repository
collection Online Access
description The present study evaluated the effect of Centella asiatica L. Urban (CA) extracts on ultrastructure and oxidative stress parameters on chronic unpredictable mild stress (CUMS) rat’s hippocampi. Rats were divided into six groups (n=6) and treated as follows: Group 1, Unstressed + normal saline (control); Group 2, CUMS + normal saline (Model); Group 3, CUMS + Flx (10 mg/kg) (FLX); Groups 4, CUMS + CA (200 mg/kg) (CA 200); Group 5, CUMS + CA (400 mg/kg) (CA 400) and Group 6, CUMS + CA (800 mg/kg) (CA 800). Nine different stressors were administered to the rats for 8 weeks consecutively, with concurrent administration of fluoxetine or CA. The hippocampi of the rats were evaluated by transmission electron microscopy (TEM) and oxidative stress biomarkers were also determined. Qualitative TEM results showed defective mitochondria, nucleus, myelin sheaths and reduced numbers of synapses in CUMS rats as well as increased oxidative stress biomarkers. Administration of CA prevented some of the structural and biochemical aberrations observed in response to stress in a manner comparable with fluoxetine, a selective serotonin-reuptake inhibitor. Conclusively, CA ameliorates structural defects induced by CUMS in the rat hippocampus, with a potential therapeutic importance in the treatment of stress and depression.
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spelling upm-1006442023-09-21T07:36:37Z http://psasir.upm.edu.my/id/eprint/100644/ Centella asiatica L. Urban protects against morphological aberrations induced by chronic unpredictable mild stress in rat’s hippocampus via attenuation of oxidative stress Jagadeesan, Saravanan Chiroma, Samaila Musa Mohd Moklas, Mohamad Aris Baharuldin, Mohamad Taufik Hidayat Mat Taib, Che Norma Amom, Zulkhairi Vishnumukkala, Thirupathirao Thomas, Warren Mahdi, Onesimus The present study evaluated the effect of Centella asiatica L. Urban (CA) extracts on ultrastructure and oxidative stress parameters on chronic unpredictable mild stress (CUMS) rat’s hippocampi. Rats were divided into six groups (n=6) and treated as follows: Group 1, Unstressed + normal saline (control); Group 2, CUMS + normal saline (Model); Group 3, CUMS + Flx (10 mg/kg) (FLX); Groups 4, CUMS + CA (200 mg/kg) (CA 200); Group 5, CUMS + CA (400 mg/kg) (CA 400) and Group 6, CUMS + CA (800 mg/kg) (CA 800). Nine different stressors were administered to the rats for 8 weeks consecutively, with concurrent administration of fluoxetine or CA. The hippocampi of the rats were evaluated by transmission electron microscopy (TEM) and oxidative stress biomarkers were also determined. Qualitative TEM results showed defective mitochondria, nucleus, myelin sheaths and reduced numbers of synapses in CUMS rats as well as increased oxidative stress biomarkers. Administration of CA prevented some of the structural and biochemical aberrations observed in response to stress in a manner comparable with fluoxetine, a selective serotonin-reuptake inhibitor. Conclusively, CA ameliorates structural defects induced by CUMS in the rat hippocampus, with a potential therapeutic importance in the treatment of stress and depression. Taylor and Francis Group 2022-07-07 Article PeerReviewed Jagadeesan, Saravanan and Chiroma, Samaila Musa and Mohd Moklas, Mohamad Aris and Baharuldin, Mohamad Taufik Hidayat and Mat Taib, Che Norma and Amom, Zulkhairi and Vishnumukkala, Thirupathirao and Thomas, Warren and Mahdi, Onesimus (2022) Centella asiatica L. Urban protects against morphological aberrations induced by chronic unpredictable mild stress in rat’s hippocampus via attenuation of oxidative stress. Egyptian Journal of Basic and Applied Sciences, 9 (1). 324 - 339. ISSN 2314-808X https://www.tandfonline.com/doi/full/10.1080/2314808X.2022.2091265 10.1080/2314808X.2022.2091265
spellingShingle Jagadeesan, Saravanan
Chiroma, Samaila Musa
Mohd Moklas, Mohamad Aris
Baharuldin, Mohamad Taufik Hidayat
Mat Taib, Che Norma
Amom, Zulkhairi
Vishnumukkala, Thirupathirao
Thomas, Warren
Mahdi, Onesimus
Centella asiatica L. Urban protects against morphological aberrations induced by chronic unpredictable mild stress in rat’s hippocampus via attenuation of oxidative stress
title Centella asiatica L. Urban protects against morphological aberrations induced by chronic unpredictable mild stress in rat’s hippocampus via attenuation of oxidative stress
title_full Centella asiatica L. Urban protects against morphological aberrations induced by chronic unpredictable mild stress in rat’s hippocampus via attenuation of oxidative stress
title_fullStr Centella asiatica L. Urban protects against morphological aberrations induced by chronic unpredictable mild stress in rat’s hippocampus via attenuation of oxidative stress
title_full_unstemmed Centella asiatica L. Urban protects against morphological aberrations induced by chronic unpredictable mild stress in rat’s hippocampus via attenuation of oxidative stress
title_short Centella asiatica L. Urban protects against morphological aberrations induced by chronic unpredictable mild stress in rat’s hippocampus via attenuation of oxidative stress
title_sort centella asiatica l. urban protects against morphological aberrations induced by chronic unpredictable mild stress in rat’s hippocampus via attenuation of oxidative stress
url http://psasir.upm.edu.my/id/eprint/100644/
http://psasir.upm.edu.my/id/eprint/100644/
http://psasir.upm.edu.my/id/eprint/100644/