Bioprospecting microalgae with the capacity for inducing calcium carbonate biomineral precipitation

Calcium carbonate precipitation is a form of sedimentary carbonate which occurs naturally in an ecosystem saturated with calcium ions and prompted by a wide assortment of microorganisms through various biological processes. The phenomenon, also known as “Microbially Induced Calcium Carbonate Precipi...

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Main Authors: Arumugam, Kavithraashree, Mohamad, Rosfarizan, Ashari, Siti Efliza, Tan, Joo Shun, Mohamed, Mohd Shamzi
Format: Article
Published: John Wiley and Sons 2022
Online Access:http://psasir.upm.edu.my/id/eprint/100548/
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author Arumugam, Kavithraashree
Mohamad, Rosfarizan
Ashari, Siti Efliza
Tan, Joo Shun
Mohamed, Mohd Shamzi
author_facet Arumugam, Kavithraashree
Mohamad, Rosfarizan
Ashari, Siti Efliza
Tan, Joo Shun
Mohamed, Mohd Shamzi
author_sort Arumugam, Kavithraashree
building UPM Institutional Repository
collection Online Access
description Calcium carbonate precipitation is a form of sedimentary carbonate which occurs naturally in an ecosystem saturated with calcium ions and prompted by a wide assortment of microorganisms through various biological processes. The phenomenon, also known as “Microbially Induced Calcium Carbonate Precipitation” (MICP), may find the characteristics of its by-product biomineral to be a promising alternative for conventional cement. Several microalgae species can produce cementitious CaCO3 deposits, which are permanently removed as solid crystal upon achieving suitable conditions for precipitation. This study assessed a total of eight microalgae for their biomineralization capability in calcification media containing 12 mM of calcium chloride dihydrate and 0.18 to 5.0 mM of sodium bicarbonate by analyzing the profiles of cell growth, pH, calcium and bicarbonate ions concentration, alkalinity, and saturation index. Among the microalgae tested, Synechocystis sp. ATCC 27178 cultured in BG-11 medium with 5.0 mM of NaHCO3 had shown the highest calcium ions removal rate (0.70 mM/day), followed by Chlorella vulgaris (0.40 mM/day). The morphology of CaCO3 crystals precipitated from these microalgae was analyzed via scanning electron microscopy (SEM) coupled with energy dispersive X-ray (EDX). The elucidation of the crystal polymorph as calcite was then confirmed through X-ray diffraction analysis.
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spelling upm-1005482023-10-10T01:21:41Z http://psasir.upm.edu.my/id/eprint/100548/ Bioprospecting microalgae with the capacity for inducing calcium carbonate biomineral precipitation Arumugam, Kavithraashree Mohamad, Rosfarizan Ashari, Siti Efliza Tan, Joo Shun Mohamed, Mohd Shamzi Calcium carbonate precipitation is a form of sedimentary carbonate which occurs naturally in an ecosystem saturated with calcium ions and prompted by a wide assortment of microorganisms through various biological processes. The phenomenon, also known as “Microbially Induced Calcium Carbonate Precipitation” (MICP), may find the characteristics of its by-product biomineral to be a promising alternative for conventional cement. Several microalgae species can produce cementitious CaCO3 deposits, which are permanently removed as solid crystal upon achieving suitable conditions for precipitation. This study assessed a total of eight microalgae for their biomineralization capability in calcification media containing 12 mM of calcium chloride dihydrate and 0.18 to 5.0 mM of sodium bicarbonate by analyzing the profiles of cell growth, pH, calcium and bicarbonate ions concentration, alkalinity, and saturation index. Among the microalgae tested, Synechocystis sp. ATCC 27178 cultured in BG-11 medium with 5.0 mM of NaHCO3 had shown the highest calcium ions removal rate (0.70 mM/day), followed by Chlorella vulgaris (0.40 mM/day). The morphology of CaCO3 crystals precipitated from these microalgae was analyzed via scanning electron microscopy (SEM) coupled with energy dispersive X-ray (EDX). The elucidation of the crystal polymorph as calcite was then confirmed through X-ray diffraction analysis. John Wiley and Sons 2022-03-23 Article PeerReviewed Arumugam, Kavithraashree and Mohamad, Rosfarizan and Ashari, Siti Efliza and Tan, Joo Shun and Mohamed, Mohd Shamzi (2022) Bioprospecting microalgae with the capacity for inducing calcium carbonate biomineral precipitation. Asia-Pacific Journal of Chemical Engineering, 17 (3). art. no. e2767. ISSN 0969-1855 https://onlinelibrary.wiley.com/doi/abs/10.1002/apj.2767 10.1002/apj.2767
spellingShingle Arumugam, Kavithraashree
Mohamad, Rosfarizan
Ashari, Siti Efliza
Tan, Joo Shun
Mohamed, Mohd Shamzi
Bioprospecting microalgae with the capacity for inducing calcium carbonate biomineral precipitation
title Bioprospecting microalgae with the capacity for inducing calcium carbonate biomineral precipitation
title_full Bioprospecting microalgae with the capacity for inducing calcium carbonate biomineral precipitation
title_fullStr Bioprospecting microalgae with the capacity for inducing calcium carbonate biomineral precipitation
title_full_unstemmed Bioprospecting microalgae with the capacity for inducing calcium carbonate biomineral precipitation
title_short Bioprospecting microalgae with the capacity for inducing calcium carbonate biomineral precipitation
title_sort bioprospecting microalgae with the capacity for inducing calcium carbonate biomineral precipitation
url http://psasir.upm.edu.my/id/eprint/100548/
http://psasir.upm.edu.my/id/eprint/100548/
http://psasir.upm.edu.my/id/eprint/100548/