Antipruritic properties of topical Channa striatus extract on stratum corneum disruption-itch mice model

Itch is an unpleasant sensation that provokes a strong desire to scratch. In some cases, antihistamines are ineffective in treating chronic itch and produce side effects. Channa striatus fish extract, which can alleviate pain, is also used in this experiment to alleviate the itch. This experiment ai...

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Main Authors: Fauzi, Irfan, Farouk, Ahmad Akira Omar, Perimal, Enoch Kumar
Format: Article
Published: Neurotak Publishing 2022
Online Access:http://psasir.upm.edu.my/id/eprint/100300/
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author Fauzi, Irfan
Farouk, Ahmad Akira Omar
Perimal, Enoch Kumar
author_facet Fauzi, Irfan
Farouk, Ahmad Akira Omar
Perimal, Enoch Kumar
author_sort Fauzi, Irfan
building UPM Institutional Repository
collection Online Access
description Itch is an unpleasant sensation that provokes a strong desire to scratch. In some cases, antihistamines are ineffective in treating chronic itch and produce side effects. Channa striatus fish extract, which can alleviate pain, is also used in this experiment to alleviate the itch. This experiment aims to determine the possible antipruritic effect of topical Channa striatus extract (CSE) on stratum corneum disruption (SCD)-itch mice model. Experiments were divided into 6 groups of male ICR mice which were positive, negative, vehicles, 3% CSE, 7% CSE, and 10% CSE groups. Rostral back of mice was induced with chemicals acetone, diethyl ether (1:1) and water (AcetoneEthylWatermethod) daily for five consecutive days. All groups were treated with specific selective treatments. The scratching behaviour of the mice was observed by videotape. The skin moisture and oiliness scoring was measured daily using a skin analyzer. The skin was isolated for RNA extraction and measured for tumour necrosis factor-alpha (TNF- α) gene expression level using real-time RT PCR. All treatment groups of Channa striatus extract cream had shown a significant reduction in scratching time, increase in skin moisture and skin oiliness. The TNF- α gene expression level had inconsistent results and needed a more extensive sample. The Channa striatus extract cream may be able to alleviate itch compared to the control parameters.
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institution Universiti Putra Malaysia
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publishDate 2022
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spelling upm-1003002024-03-18T04:08:28Z http://psasir.upm.edu.my/id/eprint/100300/ Antipruritic properties of topical Channa striatus extract on stratum corneum disruption-itch mice model Fauzi, Irfan Farouk, Ahmad Akira Omar Perimal, Enoch Kumar Itch is an unpleasant sensation that provokes a strong desire to scratch. In some cases, antihistamines are ineffective in treating chronic itch and produce side effects. Channa striatus fish extract, which can alleviate pain, is also used in this experiment to alleviate the itch. This experiment aims to determine the possible antipruritic effect of topical Channa striatus extract (CSE) on stratum corneum disruption (SCD)-itch mice model. Experiments were divided into 6 groups of male ICR mice which were positive, negative, vehicles, 3% CSE, 7% CSE, and 10% CSE groups. Rostral back of mice was induced with chemicals acetone, diethyl ether (1:1) and water (AcetoneEthylWatermethod) daily for five consecutive days. All groups were treated with specific selective treatments. The scratching behaviour of the mice was observed by videotape. The skin moisture and oiliness scoring was measured daily using a skin analyzer. The skin was isolated for RNA extraction and measured for tumour necrosis factor-alpha (TNF- α) gene expression level using real-time RT PCR. All treatment groups of Channa striatus extract cream had shown a significant reduction in scratching time, increase in skin moisture and skin oiliness. The TNF- α gene expression level had inconsistent results and needed a more extensive sample. The Channa striatus extract cream may be able to alleviate itch compared to the control parameters. Neurotak Publishing 2022-09-15 Article PeerReviewed Fauzi, Irfan and Farouk, Ahmad Akira Omar and Perimal, Enoch Kumar (2022) Antipruritic properties of topical Channa striatus extract on stratum corneum disruption-itch mice model. Neuroscience Research Notes, 5 (3). pp. 1-8. ISSN 2576-828X https://neuroscirn.org/ojs/index.php/nrnotes/article/view/145 10.31117/neuroscirn.v5i3.145
spellingShingle Fauzi, Irfan
Farouk, Ahmad Akira Omar
Perimal, Enoch Kumar
Antipruritic properties of topical Channa striatus extract on stratum corneum disruption-itch mice model
title Antipruritic properties of topical Channa striatus extract on stratum corneum disruption-itch mice model
title_full Antipruritic properties of topical Channa striatus extract on stratum corneum disruption-itch mice model
title_fullStr Antipruritic properties of topical Channa striatus extract on stratum corneum disruption-itch mice model
title_full_unstemmed Antipruritic properties of topical Channa striatus extract on stratum corneum disruption-itch mice model
title_short Antipruritic properties of topical Channa striatus extract on stratum corneum disruption-itch mice model
title_sort antipruritic properties of topical channa striatus extract on stratum corneum disruption-itch mice model
url http://psasir.upm.edu.my/id/eprint/100300/
http://psasir.upm.edu.my/id/eprint/100300/
http://psasir.upm.edu.my/id/eprint/100300/