Antifungal activity of ethanolic extract of Syzygium polyanthum (Wight) Walp. leaves extract against several types of filamentous fungi and Candida species

The aim of this study was to determine the antifungal activity of Syzygium polyanthum extract against opportunistic filamentous fungi and pathogenic Candida species by using standard method of Clinical and Laboratory Standard Institute (CLSI). All fungi were susceptible to S. polyanthum extract with...

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Main Authors: Wong, Jun Xian, Ramli, Suzita, Rukayadi, Yaya, Khir Juhari, Nik Khairunnisa, Radu, Son, Abd Wahid, Nurul Bahiyah
Format: Article
Published: Penerbit Universiti Putra Malaysia 2022
Online Access:http://psasir.upm.edu.my/id/eprint/100289/
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author Wong, Jun Xian
Ramli, Suzita
Rukayadi, Yaya
Khir Juhari, Nik Khairunnisa
Radu, Son
Abd Wahid, Nurul Bahiyah
author_facet Wong, Jun Xian
Ramli, Suzita
Rukayadi, Yaya
Khir Juhari, Nik Khairunnisa
Radu, Son
Abd Wahid, Nurul Bahiyah
author_sort Wong, Jun Xian
building UPM Institutional Repository
collection Online Access
description The aim of this study was to determine the antifungal activity of Syzygium polyanthum extract against opportunistic filamentous fungi and pathogenic Candida species by using standard method of Clinical and Laboratory Standard Institute (CLSI). All fungi were susceptible to S. polyanthum extract with minimum inhibitory concentration (MIC) and minimal fungicidal concentration (MFC) ranged between 0.63 - 1.25 mg/mL, 1.25 - 5.0 mg/mL, respectively. Moreover, the extract of S. polyanthum inhibited more ≥ 90% conidia germination of tested filamentous fungi. Killing time of S. polyanthum extract on C. albicans, C. glabrata, C. parapsilosis and C. krusei was fast acting where it showed 3 log10 CFU/mL reduction at 4× MIC in 4 h. Morphology changes had been viewed on treated strains included changing in cell wall thickness, rupture and leakage of the cells cytoplasm. The maximum cell constituents release was observed at 4× MIC for 72 h incubation for A. niger with an absorbance 0.381 whereas 0.435 for C. albicans after 4 h incubation at 4× MIC. Therefore, results suggest that S. polyanthum extract had antifungal activity and might be able to develop as a natural antifungal agent.
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spelling upm-1002892024-03-18T03:45:00Z http://psasir.upm.edu.my/id/eprint/100289/ Antifungal activity of ethanolic extract of Syzygium polyanthum (Wight) Walp. leaves extract against several types of filamentous fungi and Candida species Wong, Jun Xian Ramli, Suzita Rukayadi, Yaya Khir Juhari, Nik Khairunnisa Radu, Son Abd Wahid, Nurul Bahiyah The aim of this study was to determine the antifungal activity of Syzygium polyanthum extract against opportunistic filamentous fungi and pathogenic Candida species by using standard method of Clinical and Laboratory Standard Institute (CLSI). All fungi were susceptible to S. polyanthum extract with minimum inhibitory concentration (MIC) and minimal fungicidal concentration (MFC) ranged between 0.63 - 1.25 mg/mL, 1.25 - 5.0 mg/mL, respectively. Moreover, the extract of S. polyanthum inhibited more ≥ 90% conidia germination of tested filamentous fungi. Killing time of S. polyanthum extract on C. albicans, C. glabrata, C. parapsilosis and C. krusei was fast acting where it showed 3 log10 CFU/mL reduction at 4× MIC in 4 h. Morphology changes had been viewed on treated strains included changing in cell wall thickness, rupture and leakage of the cells cytoplasm. The maximum cell constituents release was observed at 4× MIC for 72 h incubation for A. niger with an absorbance 0.381 whereas 0.435 for C. albicans after 4 h incubation at 4× MIC. Therefore, results suggest that S. polyanthum extract had antifungal activity and might be able to develop as a natural antifungal agent. Penerbit Universiti Putra Malaysia 2022-04-19 Article PeerReviewed Wong, Jun Xian and Ramli, Suzita and Rukayadi, Yaya and Khir Juhari, Nik Khairunnisa and Radu, Son and Abd Wahid, Nurul Bahiyah (2022) Antifungal activity of ethanolic extract of Syzygium polyanthum (Wight) Walp. leaves extract against several types of filamentous fungi and Candida species. Malaysian Journal of Microscopy, 18 (1). 136 - 146. ISSN 1823-7010; ESSN: 2600-7444 https://malaysianjournalofmicroscopy.org/ojs/index.php/mjm/article/view/619
spellingShingle Wong, Jun Xian
Ramli, Suzita
Rukayadi, Yaya
Khir Juhari, Nik Khairunnisa
Radu, Son
Abd Wahid, Nurul Bahiyah
Antifungal activity of ethanolic extract of Syzygium polyanthum (Wight) Walp. leaves extract against several types of filamentous fungi and Candida species
title Antifungal activity of ethanolic extract of Syzygium polyanthum (Wight) Walp. leaves extract against several types of filamentous fungi and Candida species
title_full Antifungal activity of ethanolic extract of Syzygium polyanthum (Wight) Walp. leaves extract against several types of filamentous fungi and Candida species
title_fullStr Antifungal activity of ethanolic extract of Syzygium polyanthum (Wight) Walp. leaves extract against several types of filamentous fungi and Candida species
title_full_unstemmed Antifungal activity of ethanolic extract of Syzygium polyanthum (Wight) Walp. leaves extract against several types of filamentous fungi and Candida species
title_short Antifungal activity of ethanolic extract of Syzygium polyanthum (Wight) Walp. leaves extract against several types of filamentous fungi and Candida species
title_sort antifungal activity of ethanolic extract of syzygium polyanthum (wight) walp. leaves extract against several types of filamentous fungi and candida species
url http://psasir.upm.edu.my/id/eprint/100289/
http://psasir.upm.edu.my/id/eprint/100289/