A novel integrative methodology for research on pot-honey variations during post-harvest
This novel review of analytical methods for pot-honey research was intended to provide concise references to a 35-day post-harvest experiments at 30 °C, in an integrated study. Diverse methods were selected from specialized literature, from the AOAC (Association of Official Analytical Chemists), and...
| Main Authors: | , , , , , , , , , , , , , |
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| Format: | Article |
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Universidade Estadual de Feira de Santana
2022
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| Online Access: | http://psasir.upm.edu.my/id/eprint/100266/ |
| _version_ | 1848863278533443584 |
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| author | Vit, Patricia Chuttong, Bajaree Zawawi, Norhasnida Diaz, Maria Meulen, Jane van der Ahmad, Hajar F. Tomas-Barberan, Francisco A. Meccia, Gina Danmek, Khanchai Moreno, Jorge Enrique Roubik, David W. Barth, Ortrud Monika Lachenmeier, Dirk W. Engel, Michael S. |
| author_facet | Vit, Patricia Chuttong, Bajaree Zawawi, Norhasnida Diaz, Maria Meulen, Jane van der Ahmad, Hajar F. Tomas-Barberan, Francisco A. Meccia, Gina Danmek, Khanchai Moreno, Jorge Enrique Roubik, David W. Barth, Ortrud Monika Lachenmeier, Dirk W. Engel, Michael S. |
| author_sort | Vit, Patricia |
| building | UPM Institutional Repository |
| collection | Online Access |
| description | This novel review of analytical methods for pot-honey research was intended to provide concise references to a 35-day post-harvest experiments at 30 °C, in an integrated study. Diverse methods were selected from specialized literature, from the AOAC (Association of Official Analytical Chemists), and the International Honey Commission. Besides the geographical and seasonal origin, the pot-honey I.D. consists of entomological and botanical identifications, the latter performed by acetolyzed or natural melissopalynology. The methods of this integrative study included: 1. Physicochemical analysis (Aw, color, moisture, pH, free acidity, lactone acidity, total acidity, hydroxymethylfurfural (HMF), and sugars by highperformance liquid chromatography HPLC), 2. Targeted proton nuclear magnetic resonance 1H-NMR metabolomics (sugars, ethanol, HMF, aliphatic organic acids, amino acids, and botanical markers), 3. Biochemical composition (flavonoids, polyphenols), 4. Antioxidant activity (ABTS 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid-free radical scavenging assay, DPPH 2,2-diphenyl-1-picrylhydrazyl radical scavenging assay, ferric reduction assay FRAP), 5. Microbial counts (aerobic plate, yeast and mold, Bacillus, and lactic acid bacteria count), 6. Honey microbiome profiling via independent-culture method: high-throughput bacteria and fungi based on amplicon sequencing approaches, 7. Sensory evaluation (odor, aroma, taste, persistence), and 8. Honey authenticity and biosurfactant tests by an interphase emulsion. A further section was included to provide basic information on the results obtained using each method. This was needed to explain the interacting components derived from pot-honey processing within the stingless bee nest and post-harvest transformations. |
| first_indexed | 2025-11-15T13:30:23Z |
| format | Article |
| id | upm-100266 |
| institution | Universiti Putra Malaysia |
| institution_category | Local University |
| last_indexed | 2025-11-15T13:30:23Z |
| publishDate | 2022 |
| publisher | Universidade Estadual de Feira de Santana |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | upm-1002662024-07-09T03:44:36Z http://psasir.upm.edu.my/id/eprint/100266/ A novel integrative methodology for research on pot-honey variations during post-harvest Vit, Patricia Chuttong, Bajaree Zawawi, Norhasnida Diaz, Maria Meulen, Jane van der Ahmad, Hajar F. Tomas-Barberan, Francisco A. Meccia, Gina Danmek, Khanchai Moreno, Jorge Enrique Roubik, David W. Barth, Ortrud Monika Lachenmeier, Dirk W. Engel, Michael S. This novel review of analytical methods for pot-honey research was intended to provide concise references to a 35-day post-harvest experiments at 30 °C, in an integrated study. Diverse methods were selected from specialized literature, from the AOAC (Association of Official Analytical Chemists), and the International Honey Commission. Besides the geographical and seasonal origin, the pot-honey I.D. consists of entomological and botanical identifications, the latter performed by acetolyzed or natural melissopalynology. The methods of this integrative study included: 1. Physicochemical analysis (Aw, color, moisture, pH, free acidity, lactone acidity, total acidity, hydroxymethylfurfural (HMF), and sugars by highperformance liquid chromatography HPLC), 2. Targeted proton nuclear magnetic resonance 1H-NMR metabolomics (sugars, ethanol, HMF, aliphatic organic acids, amino acids, and botanical markers), 3. Biochemical composition (flavonoids, polyphenols), 4. Antioxidant activity (ABTS 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid-free radical scavenging assay, DPPH 2,2-diphenyl-1-picrylhydrazyl radical scavenging assay, ferric reduction assay FRAP), 5. Microbial counts (aerobic plate, yeast and mold, Bacillus, and lactic acid bacteria count), 6. Honey microbiome profiling via independent-culture method: high-throughput bacteria and fungi based on amplicon sequencing approaches, 7. Sensory evaluation (odor, aroma, taste, persistence), and 8. Honey authenticity and biosurfactant tests by an interphase emulsion. A further section was included to provide basic information on the results obtained using each method. This was needed to explain the interacting components derived from pot-honey processing within the stingless bee nest and post-harvest transformations. Universidade Estadual de Feira de Santana 2022-11-28 Article PeerReviewed Vit, Patricia and Chuttong, Bajaree and Zawawi, Norhasnida and Diaz, Maria and Meulen, Jane van der and Ahmad, Hajar F. and Tomas-Barberan, Francisco A. and Meccia, Gina and Danmek, Khanchai and Moreno, Jorge Enrique and Roubik, David W. and Barth, Ortrud Monika and Lachenmeier, Dirk W. and Engel, Michael S. (2022) A novel integrative methodology for research on pot-honey variations during post-harvest. Sociobiology: An international journal on social insects, 69 (4). art. no. 8251. pp. 1-27. ISSN 0361-6525 https://periodicos.uefs.br/index.php/sociobiology/article/view/8251 10.13102/sociobiology.v69i4.8251 |
| spellingShingle | Vit, Patricia Chuttong, Bajaree Zawawi, Norhasnida Diaz, Maria Meulen, Jane van der Ahmad, Hajar F. Tomas-Barberan, Francisco A. Meccia, Gina Danmek, Khanchai Moreno, Jorge Enrique Roubik, David W. Barth, Ortrud Monika Lachenmeier, Dirk W. Engel, Michael S. A novel integrative methodology for research on pot-honey variations during post-harvest |
| title | A novel integrative methodology for research on pot-honey variations during post-harvest |
| title_full | A novel integrative methodology for research on pot-honey variations during post-harvest |
| title_fullStr | A novel integrative methodology for research on pot-honey variations during post-harvest |
| title_full_unstemmed | A novel integrative methodology for research on pot-honey variations during post-harvest |
| title_short | A novel integrative methodology for research on pot-honey variations during post-harvest |
| title_sort | novel integrative methodology for research on pot-honey variations during post-harvest |
| url | http://psasir.upm.edu.my/id/eprint/100266/ http://psasir.upm.edu.my/id/eprint/100266/ http://psasir.upm.edu.my/id/eprint/100266/ |