Antagonistic Activity of Endophytic Bacillus Species Against Colletotrichum Gloeosporioides for the Control of Anthracnose Disease in Black Pepper (Piper Nigrum L.)
Anthracnose caused by Collectotrichum gloeosporioides is a serious disease of black pepper. The antagonistic effect of three Bacillus species, i.e. Bacillus strain CBF, YCA0098 and YCA5593, were tested against Collectotrichum gloeosporioides in vitro and in vivo. In vitro test showed that all Baci...
| Main Authors: | , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Researchgate
2015
|
| Subjects: | |
| Online Access: | http://ir.unimas.my/id/eprint/9972/ http://ir.unimas.my/id/eprint/9972/1/Antagonistic%20Activity%20of%20Endophytic%20Bacillus%20Species%20Against%20Colletotrichum%20Gloeosporioides%20for%20the%20Control%20of%20Anthracnose%20Disease%20in%20Black%20Pepper%20%28Piper%20Nigrum%20L.%29%20%28abstract%29.pdf |
| _version_ | 1848836684116918272 |
|---|---|
| author | Yap, Chin Ann Awang Ahmad Sallehin, Awang Husaini Hairul, Azman Roslan Mohd Hasnain, Hussain Samuel, Lihan |
| author_facet | Yap, Chin Ann Awang Ahmad Sallehin, Awang Husaini Hairul, Azman Roslan Mohd Hasnain, Hussain Samuel, Lihan |
| author_sort | Yap, Chin Ann |
| building | UNIMAS Institutional Repository |
| collection | Online Access |
| description | Anthracnose caused by Collectotrichum gloeosporioides is a serious disease of black pepper. The antagonistic
effect of three Bacillus species, i.e. Bacillus strain CBF, YCA0098 and YCA5593, were tested against Collectotrichum
gloeosporioides in vitro and in vivo. In vitro test showed that all Bacillus species significantly reduce the mycelia growth
and spore germination of the C. gloeosporioides. Scanning electron microscopy revealed significant inhibition of C.
gloeosporioides spore germination on pepper leaves surface. Combination of bacterial strain CBF, YCA0098 and
YCA5593 maintained but not increased the inhibitory effect on spore germination of C. gloeosporioides when cells were
co-incubated with C. gloeosporioides and when the pathogen was incubated in mixture of cell-free culture extracts.
Combination of strains maintained efficacy in control of C. gloeosporioides in pepper vines compared to individual
strains, but reduced variability and improved consistency between experiments, especially mixture of strain CBF,
YCA0098 and YCA5593. |
| first_indexed | 2025-11-15T06:27:40Z |
| format | Article |
| id | unimas-9972 |
| institution | Universiti Malaysia Sarawak |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-15T06:27:40Z |
| publishDate | 2015 |
| publisher | Researchgate |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | unimas-99722015-12-08T03:04:03Z http://ir.unimas.my/id/eprint/9972/ Antagonistic Activity of Endophytic Bacillus Species Against Colletotrichum Gloeosporioides for the Control of Anthracnose Disease in Black Pepper (Piper Nigrum L.) Yap, Chin Ann Awang Ahmad Sallehin, Awang Husaini Hairul, Azman Roslan Mohd Hasnain, Hussain Samuel, Lihan S Agriculture (General) Anthracnose caused by Collectotrichum gloeosporioides is a serious disease of black pepper. The antagonistic effect of three Bacillus species, i.e. Bacillus strain CBF, YCA0098 and YCA5593, were tested against Collectotrichum gloeosporioides in vitro and in vivo. In vitro test showed that all Bacillus species significantly reduce the mycelia growth and spore germination of the C. gloeosporioides. Scanning electron microscopy revealed significant inhibition of C. gloeosporioides spore germination on pepper leaves surface. Combination of bacterial strain CBF, YCA0098 and YCA5593 maintained but not increased the inhibitory effect on spore germination of C. gloeosporioides when cells were co-incubated with C. gloeosporioides and when the pathogen was incubated in mixture of cell-free culture extracts. Combination of strains maintained efficacy in control of C. gloeosporioides in pepper vines compared to individual strains, but reduced variability and improved consistency between experiments, especially mixture of strain CBF, YCA0098 and YCA5593. Researchgate 2015 Article NonPeerReviewed text en http://ir.unimas.my/id/eprint/9972/1/Antagonistic%20Activity%20of%20Endophytic%20Bacillus%20Species%20Against%20Colletotrichum%20Gloeosporioides%20for%20the%20Control%20of%20Anthracnose%20Disease%20in%20Black%20Pepper%20%28Piper%20Nigrum%20L.%29%20%28abstract%29.pdf Yap, Chin Ann and Awang Ahmad Sallehin, Awang Husaini and Hairul, Azman Roslan and Mohd Hasnain, Hussain and Samuel, Lihan (2015) Antagonistic Activity of Endophytic Bacillus Species Against Colletotrichum Gloeosporioides for the Control of Anthracnose Disease in Black Pepper (Piper Nigrum L.). Global Journal of Biology, Agriculture and Health Sciences, 4 (2). pp. 115-123. ISSN 2319 – 5584 http://www.researchgate.net/publication/280808054_Antagonistic_Activity_of_Endophytic_Bacillus_Species_Against_Colletotrichum_Gloeosporioides_for_the_Control_of_Anthracnose_Disease_in_Black_Pepper_(Piper_Nigrum_L.) |
| spellingShingle | S Agriculture (General) Yap, Chin Ann Awang Ahmad Sallehin, Awang Husaini Hairul, Azman Roslan Mohd Hasnain, Hussain Samuel, Lihan Antagonistic Activity of Endophytic Bacillus Species Against Colletotrichum Gloeosporioides for the Control of Anthracnose Disease in Black Pepper (Piper Nigrum L.) |
| title | Antagonistic Activity of Endophytic Bacillus Species Against Colletotrichum Gloeosporioides for the Control of Anthracnose Disease in Black Pepper (Piper Nigrum L.) |
| title_full | Antagonistic Activity of Endophytic Bacillus Species Against Colletotrichum Gloeosporioides for the Control of Anthracnose Disease in Black Pepper (Piper Nigrum L.) |
| title_fullStr | Antagonistic Activity of Endophytic Bacillus Species Against Colletotrichum Gloeosporioides for the Control of Anthracnose Disease in Black Pepper (Piper Nigrum L.) |
| title_full_unstemmed | Antagonistic Activity of Endophytic Bacillus Species Against Colletotrichum Gloeosporioides for the Control of Anthracnose Disease in Black Pepper (Piper Nigrum L.) |
| title_short | Antagonistic Activity of Endophytic Bacillus Species Against Colletotrichum Gloeosporioides for the Control of Anthracnose Disease in Black Pepper (Piper Nigrum L.) |
| title_sort | antagonistic activity of endophytic bacillus species against colletotrichum gloeosporioides for the control of anthracnose disease in black pepper (piper nigrum l.) |
| topic | S Agriculture (General) |
| url | http://ir.unimas.my/id/eprint/9972/ http://ir.unimas.my/id/eprint/9972/ http://ir.unimas.my/id/eprint/9972/1/Antagonistic%20Activity%20of%20Endophytic%20Bacillus%20Species%20Against%20Colletotrichum%20Gloeosporioides%20for%20the%20Control%20of%20Anthracnose%20Disease%20in%20Black%20Pepper%20%28Piper%20Nigrum%20L.%29%20%28abstract%29.pdf |