Effects of Extracts and Fractions of Gynura procumbens on Rat Atrial Contraction
There is currently a great deal of research interest in utilizing plant compounds against human diseases, including hypertension. The present study investigated the effects of different extracts and fractions from leaves of Gynura procumbens Merr. on rat atrial contraction in vitro. Isolated left an...
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International Pharmacopuncture Institute
2013
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| Online Access: | http://ir.unimas.my/id/eprint/9869/ http://ir.unimas.my/id/eprint/9869/1/Omar%20Saad.pdf |
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| author | Omar Saad, Saleh Abrika Yam, Mun Fei Mohd. Zaini, Asmawi Amirin, Sadikun Dieng, Hamady Elssanousi, Ali Hussain |
| author_facet | Omar Saad, Saleh Abrika Yam, Mun Fei Mohd. Zaini, Asmawi Amirin, Sadikun Dieng, Hamady Elssanousi, Ali Hussain |
| author_sort | Omar Saad, Saleh Abrika |
| building | UNIMAS Institutional Repository |
| collection | Online Access |
| description | There is currently a great deal of research interest in utilizing plant compounds against human diseases, including hypertension. The present study investigated the effects of different extracts and fractions from leaves of Gynura procumbens Merr. on rat atrial contraction in vitro. Isolated left and right atria, mounted in a 20-ml organ bath, were allowed to equilibrate for 15 min before the application of the extracts or fractions. The extracts (petroleum-ether extract (PE) and methanol extract (ME)) and the fractions (chloroform fraction (CHL), ethyl-acetate fraction (EA), n-butanol fraction (NB) and water fraction (WA) of the methanol extract) were tested at three concentrations (0.25, 0.5 and 1.0 mg/ml), with a b-adrenergic agonist (isoprenaline) as a control. All data on contraction responses were log-transformed and analyzed. When exposed to the different extracts, both atria tended
to exhibit greater contractive responses with the NB whereas cardiac contractions had a tendency to be reduced with most other extracts. For a given extract, the contraction responses were particularly greater at 0.5 mg/ml for the right atrium and at 1 mg/ml for the left atrium. Further analysis focusing on the NB fraction revealed that positive inotropism was greater in left atria exposed to highly-concentrated F2 and F3 sub-fractions. Taken together,
our results suggest that NB extracts and fractions from the G. procumbens-leaf methanol extract have positive inotropic activities and, hence, can be considered as an alternative/traditional medicine against increased blood pressure in humans or can be used in strategies aimed at finding antihypertensive biomolecules from an accessible source. |
| first_indexed | 2025-11-15T06:27:12Z |
| format | Article |
| id | unimas-9869 |
| institution | Universiti Malaysia Sarawak |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-15T06:27:12Z |
| publishDate | 2013 |
| publisher | International Pharmacopuncture Institute |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | unimas-98692022-01-25T07:47:36Z http://ir.unimas.my/id/eprint/9869/ Effects of Extracts and Fractions of Gynura procumbens on Rat Atrial Contraction Omar Saad, Saleh Abrika Yam, Mun Fei Mohd. Zaini, Asmawi Amirin, Sadikun Dieng, Hamady Elssanousi, Ali Hussain QK Botany There is currently a great deal of research interest in utilizing plant compounds against human diseases, including hypertension. The present study investigated the effects of different extracts and fractions from leaves of Gynura procumbens Merr. on rat atrial contraction in vitro. Isolated left and right atria, mounted in a 20-ml organ bath, were allowed to equilibrate for 15 min before the application of the extracts or fractions. The extracts (petroleum-ether extract (PE) and methanol extract (ME)) and the fractions (chloroform fraction (CHL), ethyl-acetate fraction (EA), n-butanol fraction (NB) and water fraction (WA) of the methanol extract) were tested at three concentrations (0.25, 0.5 and 1.0 mg/ml), with a b-adrenergic agonist (isoprenaline) as a control. All data on contraction responses were log-transformed and analyzed. When exposed to the different extracts, both atria tended to exhibit greater contractive responses with the NB whereas cardiac contractions had a tendency to be reduced with most other extracts. For a given extract, the contraction responses were particularly greater at 0.5 mg/ml for the right atrium and at 1 mg/ml for the left atrium. Further analysis focusing on the NB fraction revealed that positive inotropism was greater in left atria exposed to highly-concentrated F2 and F3 sub-fractions. Taken together, our results suggest that NB extracts and fractions from the G. procumbens-leaf methanol extract have positive inotropic activities and, hence, can be considered as an alternative/traditional medicine against increased blood pressure in humans or can be used in strategies aimed at finding antihypertensive biomolecules from an accessible source. International Pharmacopuncture Institute 2013 Article NonPeerReviewed text en http://ir.unimas.my/id/eprint/9869/1/Omar%20Saad.pdf Omar Saad, Saleh Abrika and Yam, Mun Fei and Mohd. Zaini, Asmawi and Amirin, Sadikun and Dieng, Hamady and Elssanousi, Ali Hussain (2013) Effects of Extracts and Fractions of Gynura procumbens on Rat Atrial Contraction. Journal of Acupuncture and Meridian Studies, 6 (4). pp. 199-207. ISSN 2005-2901 http://ac.els-cdn.com/S2005290113000216/1-s2.0-S2005290113000216-main.pdf?_tid=e2208d14-9287-11e5-96cc-00000aab0f6c&acdnat=1448354981_68ab54a448fb5e02b1e8ebb371588e9b 10.1016/j.jams.2013.01.020 |
| spellingShingle | QK Botany Omar Saad, Saleh Abrika Yam, Mun Fei Mohd. Zaini, Asmawi Amirin, Sadikun Dieng, Hamady Elssanousi, Ali Hussain Effects of Extracts and Fractions of Gynura procumbens on Rat Atrial Contraction |
| title | Effects of Extracts and Fractions of Gynura procumbens on Rat Atrial Contraction |
| title_full | Effects of Extracts and Fractions of Gynura procumbens on Rat Atrial Contraction |
| title_fullStr | Effects of Extracts and Fractions of Gynura procumbens on Rat Atrial Contraction |
| title_full_unstemmed | Effects of Extracts and Fractions of Gynura procumbens on Rat Atrial Contraction |
| title_short | Effects of Extracts and Fractions of Gynura procumbens on Rat Atrial Contraction |
| title_sort | effects of extracts and fractions of gynura procumbens on rat atrial contraction |
| topic | QK Botany |
| url | http://ir.unimas.my/id/eprint/9869/ http://ir.unimas.my/id/eprint/9869/ http://ir.unimas.my/id/eprint/9869/ http://ir.unimas.my/id/eprint/9869/1/Omar%20Saad.pdf |