Xylanase Gene from a Locally Isolated Bacterium
A xylanolytic bacterium was isolated from sago plantation humus. Results from the morphological observation, biochemical tests and 16s rRNA sequencing suggested the bacterium to be Klebsiella pneumoniae. Due to the xylanolytic activity of this bacterium, isolation and characterization of the xylanas...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
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Malaysian Applied Biology Journal
2011
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| Online Access: | http://ir.unimas.my/id/eprint/9832/ http://ir.unimas.my/id/eprint/9832/1/XYLANASE%20GENE%20FROM%20A%20LOCALLY%20ISOLATED%20BACTERIUM%28abstract%29.pdf |
| _version_ | 1848836646201458688 |
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| author | Hussain, MH Chong, Nikson Fatt Ming Chan, CSW Safarina, A Husaini, A. |
| author_facet | Hussain, MH Chong, Nikson Fatt Ming Chan, CSW Safarina, A Husaini, A. |
| author_sort | Hussain, MH |
| building | UNIMAS Institutional Repository |
| collection | Online Access |
| description | A xylanolytic bacterium was isolated from sago plantation humus. Results from the morphological observation, biochemical tests and 16s rRNA sequencing suggested the bacterium to be Klebsiella pneumoniae. Due to the xylanolytic activity of this bacterium, isolation and characterization of the xylanase gene were attempted. A distinct fragment of about 650 bp was successfully amplified using PCR and cloned into Escherichia coli XL-1 Blue. A BLAST search confirmed that the DNA sequence from the amplified fragment was endo-1,4-beta-xylanase gene from the family 11 glycoside hydrolase. It showed 98% homology with Bacillus subtilis xylanase gene. In silico characterisation showed an open reading frame encoding a 213 amino acid sequence with a molecular weight of 23.3 kDa and theoretical isolectric point (pI) at pH 9.42. |
| first_indexed | 2025-11-15T06:27:04Z |
| format | Article |
| id | unimas-9832 |
| institution | Universiti Malaysia Sarawak |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-15T06:27:04Z |
| publishDate | 2011 |
| publisher | Malaysian Applied Biology Journal |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | unimas-98322016-04-14T01:29:44Z http://ir.unimas.my/id/eprint/9832/ Xylanase Gene from a Locally Isolated Bacterium Hussain, MH Chong, Nikson Fatt Ming Chan, CSW Safarina, A Husaini, A. Q Science (General) QR Microbiology A xylanolytic bacterium was isolated from sago plantation humus. Results from the morphological observation, biochemical tests and 16s rRNA sequencing suggested the bacterium to be Klebsiella pneumoniae. Due to the xylanolytic activity of this bacterium, isolation and characterization of the xylanase gene were attempted. A distinct fragment of about 650 bp was successfully amplified using PCR and cloned into Escherichia coli XL-1 Blue. A BLAST search confirmed that the DNA sequence from the amplified fragment was endo-1,4-beta-xylanase gene from the family 11 glycoside hydrolase. It showed 98% homology with Bacillus subtilis xylanase gene. In silico characterisation showed an open reading frame encoding a 213 amino acid sequence with a molecular weight of 23.3 kDa and theoretical isolectric point (pI) at pH 9.42. Malaysian Applied Biology Journal 2011 Article NonPeerReviewed text en http://ir.unimas.my/id/eprint/9832/1/XYLANASE%20GENE%20FROM%20A%20LOCALLY%20ISOLATED%20BACTERIUM%28abstract%29.pdf Hussain, MH and Chong, Nikson Fatt Ming and Chan, CSW and Safarina, A and Husaini, A. (2011) Xylanase Gene from a Locally Isolated Bacterium. Malaysian Applied Biology Journal, 40 (1). pp. 33-38. ISSN 0126-8643 http://mabjournal.com/images/40_1_June_2011/06_hasnain.pdf |
| spellingShingle | Q Science (General) QR Microbiology Hussain, MH Chong, Nikson Fatt Ming Chan, CSW Safarina, A Husaini, A. Xylanase Gene from a Locally Isolated Bacterium |
| title | Xylanase Gene from a Locally Isolated Bacterium |
| title_full | Xylanase Gene from a Locally Isolated Bacterium |
| title_fullStr | Xylanase Gene from a Locally Isolated Bacterium |
| title_full_unstemmed | Xylanase Gene from a Locally Isolated Bacterium |
| title_short | Xylanase Gene from a Locally Isolated Bacterium |
| title_sort | xylanase gene from a locally isolated bacterium |
| topic | Q Science (General) QR Microbiology |
| url | http://ir.unimas.my/id/eprint/9832/ http://ir.unimas.my/id/eprint/9832/ http://ir.unimas.my/id/eprint/9832/1/XYLANASE%20GENE%20FROM%20A%20LOCALLY%20ISOLATED%20BACTERIUM%28abstract%29.pdf |