Preliminary Gene Characterization of α-Amylase from Bacillus amyloliquefaciens UMAS 1002
Characterization of α-amylase gene sequence produced by Bacillus amyloliquefaciens UMAS 1002, a cellulolytic and amylolytic bacilli isolated from sago pith waste is described here. The amyE gene encoding the α-amylase was isolated by polymerase chain reaction. The 1,980 bp of amyE gene corresponding...
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| Format: | Article |
| Language: | English |
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Universiti Malaysia Sarawak, (UNIMAS)
2013
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| Online Access: | http://ir.unimas.my/id/eprint/9829/ http://ir.unimas.my/id/eprint/9829/1/Preliminary.pdf |
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| author | Muhammad Suhaib, M. H. Mohd Hasnain, Hussain Awang Ahmad Sallehin, Awang Husaini Kopli, Bujang Dayang Salwani, Awang Adeni Mohd Azib, Salleh |
| author_facet | Muhammad Suhaib, M. H. Mohd Hasnain, Hussain Awang Ahmad Sallehin, Awang Husaini Kopli, Bujang Dayang Salwani, Awang Adeni Mohd Azib, Salleh |
| author_sort | Muhammad Suhaib, M. H. |
| building | UNIMAS Institutional Repository |
| collection | Online Access |
| description | Characterization of α-amylase gene sequence produced by Bacillus amyloliquefaciens UMAS 1002, a cellulolytic and amylolytic bacilli isolated from sago pith waste is described here. The amyE gene encoding the α-amylase was isolated by polymerase chain reaction. The 1,980 bp of amyE gene corresponding to 660 amino acids showed 99% homology to the α-amylase sequence from Bacillus subtilis X-23 (GenBank: BAA31528). The α-amylase sequence of B. amyloliquefaciens UMAS 1002 (GenBank: KC800929) differs from that of B. subtilis X-23 by 5 amino acids. In silico analysis of α-amylase from B. amyloliquefaciens UMAS 1002 showed similar characteristics compared to α-amylase from B. subtilis X-23 |
| first_indexed | 2025-11-15T06:27:03Z |
| format | Article |
| id | unimas-9829 |
| institution | Universiti Malaysia Sarawak |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-15T06:27:03Z |
| publishDate | 2013 |
| publisher | Universiti Malaysia Sarawak, (UNIMAS) |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | unimas-98292022-01-25T07:33:28Z http://ir.unimas.my/id/eprint/9829/ Preliminary Gene Characterization of α-Amylase from Bacillus amyloliquefaciens UMAS 1002 Muhammad Suhaib, M. H. Mohd Hasnain, Hussain Awang Ahmad Sallehin, Awang Husaini Kopli, Bujang Dayang Salwani, Awang Adeni Mohd Azib, Salleh Q Science (General) QR Microbiology Characterization of α-amylase gene sequence produced by Bacillus amyloliquefaciens UMAS 1002, a cellulolytic and amylolytic bacilli isolated from sago pith waste is described here. The amyE gene encoding the α-amylase was isolated by polymerase chain reaction. The 1,980 bp of amyE gene corresponding to 660 amino acids showed 99% homology to the α-amylase sequence from Bacillus subtilis X-23 (GenBank: BAA31528). The α-amylase sequence of B. amyloliquefaciens UMAS 1002 (GenBank: KC800929) differs from that of B. subtilis X-23 by 5 amino acids. In silico analysis of α-amylase from B. amyloliquefaciens UMAS 1002 showed similar characteristics compared to α-amylase from B. subtilis X-23 Universiti Malaysia Sarawak, (UNIMAS) 2013 Article NonPeerReviewed text en http://ir.unimas.my/id/eprint/9829/1/Preliminary.pdf Muhammad Suhaib, M. H. and Mohd Hasnain, Hussain and Awang Ahmad Sallehin, Awang Husaini and Kopli, Bujang and Dayang Salwani, Awang Adeni and Mohd Azib, Salleh (2013) Preliminary Gene Characterization of α-Amylase from Bacillus amyloliquefaciens UMAS 1002. Borneo Journal of Resource Science and Technology, 3 (2). pp. 36-39. ISSN 229-9769 http://www.researchgate.net/publication/280813911 |
| spellingShingle | Q Science (General) QR Microbiology Muhammad Suhaib, M. H. Mohd Hasnain, Hussain Awang Ahmad Sallehin, Awang Husaini Kopli, Bujang Dayang Salwani, Awang Adeni Mohd Azib, Salleh Preliminary Gene Characterization of α-Amylase from Bacillus amyloliquefaciens UMAS 1002 |
| title | Preliminary Gene Characterization of α-Amylase from Bacillus amyloliquefaciens UMAS 1002 |
| title_full | Preliminary Gene Characterization of α-Amylase from Bacillus amyloliquefaciens UMAS 1002 |
| title_fullStr | Preliminary Gene Characterization of α-Amylase from Bacillus amyloliquefaciens UMAS 1002 |
| title_full_unstemmed | Preliminary Gene Characterization of α-Amylase from Bacillus amyloliquefaciens UMAS 1002 |
| title_short | Preliminary Gene Characterization of α-Amylase from Bacillus amyloliquefaciens UMAS 1002 |
| title_sort | preliminary gene characterization of α-amylase from bacillus amyloliquefaciens umas 1002 |
| topic | Q Science (General) QR Microbiology |
| url | http://ir.unimas.my/id/eprint/9829/ http://ir.unimas.my/id/eprint/9829/ http://ir.unimas.my/id/eprint/9829/1/Preliminary.pdf |