Bioremediation of PCP by Trichoderma and Cunninghamella Strains Isolated from Sawdust

Four fungal isolates, SD12, SD14, SD19 and SD20 isolated from the aged sawdust grew on agar plates supplemented with PCP up to a concentration of 100 mg L-1. At high PCP concentration, isolate SD12 showed the highest radial growth rate of 10 mm day-1, followed by SD14 and SD19 both with 4.5 mm day-1...

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Main Authors: Sing, Ngieng Ngui, Azham, Zulkharnain, Hairul, Azman Roslan, Zani, Assim, Ahmad, Husaini
Format: Article
Language:English
Published: Scielo 2014
Subjects:
Online Access:http://ir.unimas.my/id/eprint/9807/
http://ir.unimas.my/id/eprint/9807/1/Zulkharnain.pdf
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author Sing, Ngieng Ngui
Azham, Zulkharnain
Hairul, Azman Roslan
Zani, Assim
Ahmad, Husaini
author_facet Sing, Ngieng Ngui
Azham, Zulkharnain
Hairul, Azman Roslan
Zani, Assim
Ahmad, Husaini
author_sort Sing, Ngieng Ngui
building UNIMAS Institutional Repository
collection Online Access
description Four fungal isolates, SD12, SD14, SD19 and SD20 isolated from the aged sawdust grew on agar plates supplemented with PCP up to a concentration of 100 mg L-1. At high PCP concentration, isolate SD12 showed the highest radial growth rate of 10 mm day-1, followed by SD14 and SD19 both with 4.5 mm day-1 and SD20 with 4.2 mm day-1. Ultrastructural study on the effect of PCP on the PCP tolerant fungi using scanning electron microscope showed that high concentration of PCP caused the collapse of both fungal hyphae and spores. Among the four PCP tolerant fungi examined, isolate SD12 showed the least structural damage at high PCP concentration of 100 mg L-1. This fungal isolate was further characterized and identified as Cunninghamella sp. UMAS SD12. Preliminary PCP biodegradation trial performed in liquid minimal medium supplemented with 20 mg L-1 of PCP using Cunninghamella sp. UMAS SD12 showed that the degradation up to 51.7% of PCP in 15 days under static growth condition.
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spelling unimas-98072021-06-29T17:19:47Z http://ir.unimas.my/id/eprint/9807/ Bioremediation of PCP by Trichoderma and Cunninghamella Strains Isolated from Sawdust Sing, Ngieng Ngui Azham, Zulkharnain Hairul, Azman Roslan Zani, Assim Ahmad, Husaini Q Science (General) QD Chemistry Four fungal isolates, SD12, SD14, SD19 and SD20 isolated from the aged sawdust grew on agar plates supplemented with PCP up to a concentration of 100 mg L-1. At high PCP concentration, isolate SD12 showed the highest radial growth rate of 10 mm day-1, followed by SD14 and SD19 both with 4.5 mm day-1 and SD20 with 4.2 mm day-1. Ultrastructural study on the effect of PCP on the PCP tolerant fungi using scanning electron microscope showed that high concentration of PCP caused the collapse of both fungal hyphae and spores. Among the four PCP tolerant fungi examined, isolate SD12 showed the least structural damage at high PCP concentration of 100 mg L-1. This fungal isolate was further characterized and identified as Cunninghamella sp. UMAS SD12. Preliminary PCP biodegradation trial performed in liquid minimal medium supplemented with 20 mg L-1 of PCP using Cunninghamella sp. UMAS SD12 showed that the degradation up to 51.7% of PCP in 15 days under static growth condition. Scielo 2014 Article PeerReviewed text en http://ir.unimas.my/id/eprint/9807/1/Zulkharnain.pdf Sing, Ngieng Ngui and Azham, Zulkharnain and Hairul, Azman Roslan and Zani, Assim and Ahmad, Husaini (2014) Bioremediation of PCP by Trichoderma and Cunninghamella Strains Isolated from Sawdust. Brazilian Archives of Biology and Technology, 57 (6). pp. 811-820. ISSN 1516-8913 http://www.scielo.br/pdf/babt/v57n6/1516-8913-babt-57-06-00811.pdf http://dx.doi.org/10.1590/S1516-8913201402852
spellingShingle Q Science (General)
QD Chemistry
Sing, Ngieng Ngui
Azham, Zulkharnain
Hairul, Azman Roslan
Zani, Assim
Ahmad, Husaini
Bioremediation of PCP by Trichoderma and Cunninghamella Strains Isolated from Sawdust
title Bioremediation of PCP by Trichoderma and Cunninghamella Strains Isolated from Sawdust
title_full Bioremediation of PCP by Trichoderma and Cunninghamella Strains Isolated from Sawdust
title_fullStr Bioremediation of PCP by Trichoderma and Cunninghamella Strains Isolated from Sawdust
title_full_unstemmed Bioremediation of PCP by Trichoderma and Cunninghamella Strains Isolated from Sawdust
title_short Bioremediation of PCP by Trichoderma and Cunninghamella Strains Isolated from Sawdust
title_sort bioremediation of pcp by trichoderma and cunninghamella strains isolated from sawdust
topic Q Science (General)
QD Chemistry
url http://ir.unimas.my/id/eprint/9807/
http://ir.unimas.my/id/eprint/9807/
http://ir.unimas.my/id/eprint/9807/
http://ir.unimas.my/id/eprint/9807/1/Zulkharnain.pdf