Development and characterization of microsatellite markers in sawih tree (Duabanga moluccana Blume) using ISSR-suppression PCR techniques.
Duabanga moluccana(or locally known as sawih)is an indigenous fast growing tropical tree species that confersvarious advantages for the timber industry and for plantedforests development. In this paper, we isolated and characterized8 polymorphic microsatellite markers from theD. moluccanagenome usin...
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| Format: | Article |
| Language: | English |
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Springer
2015
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| Online Access: | http://ir.unimas.my/id/eprint/9756/ http://ir.unimas.my/id/eprint/9756/1/Kit%20Siong.pdf |
| _version_ | 1848836632825823232 |
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| author | Liew, Kit-Siong Ho, Wei-Seng Pang, Shek-Ling Abdullah, Julaihi |
| author_facet | Liew, Kit-Siong Ho, Wei-Seng Pang, Shek-Ling Abdullah, Julaihi |
| author_sort | Liew, Kit-Siong |
| building | UNIMAS Institutional Repository |
| collection | Online Access |
| description | Duabanga moluccana(or locally known as sawih)is an indigenous fast growing tropical tree species that confersvarious advantages for the timber industry and for plantedforests development. In this paper, we isolated and characterized8 polymorphic microsatellite markers from theD. moluccanagenome using ISSR-suppression PCR techniques. The numberof alleles and PIC values ranged from 3 to 8 alleles per locus andfrom 0.488 to 0.792, respectively. Three microsatellite loci weredeviated from Hardy-Weinberg equilibrium (P<0.05). Thetransferability rate ranged from 24 to 100 % among the threeindigenous tree species tested. This indicates that the newlydeveloped microsatellite markers would be useful tools forpopulation genetic studies onD. moluccanaand otherindigenous tree species. |
| first_indexed | 2025-11-15T06:26:51Z |
| format | Article |
| id | unimas-9756 |
| institution | Universiti Malaysia Sarawak |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-15T06:26:51Z |
| publishDate | 2015 |
| publisher | Springer |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | unimas-97562022-01-28T00:34:08Z http://ir.unimas.my/id/eprint/9756/ Development and characterization of microsatellite markers in sawih tree (Duabanga moluccana Blume) using ISSR-suppression PCR techniques. Liew, Kit-Siong Ho, Wei-Seng Pang, Shek-Ling Abdullah, Julaihi Q Science (General) QK Botany Duabanga moluccana(or locally known as sawih)is an indigenous fast growing tropical tree species that confersvarious advantages for the timber industry and for plantedforests development. In this paper, we isolated and characterized8 polymorphic microsatellite markers from theD. moluccanagenome using ISSR-suppression PCR techniques. The numberof alleles and PIC values ranged from 3 to 8 alleles per locus andfrom 0.488 to 0.792, respectively. Three microsatellite loci weredeviated from Hardy-Weinberg equilibrium (P<0.05). Thetransferability rate ranged from 24 to 100 % among the threeindigenous tree species tested. This indicates that the newlydeveloped microsatellite markers would be useful tools forpopulation genetic studies onD. moluccanaand otherindigenous tree species. Springer 2015 Article PeerReviewed text en http://ir.unimas.my/id/eprint/9756/1/Kit%20Siong.pdf Liew, Kit-Siong and Ho, Wei-Seng and Pang, Shek-Ling and Abdullah, Julaihi (2015) Development and characterization of microsatellite markers in sawih tree (Duabanga moluccana Blume) using ISSR-suppression PCR techniques. Physiology and Molecular Biology of Plants, 21 (1). pp. 163-165. ISSN 0971-5894 http://link.springer.com/article/10.1007%2Fs12298-014-0262-2#page-1 DOI 10.1007/s12298-014-0262-2 |
| spellingShingle | Q Science (General) QK Botany Liew, Kit-Siong Ho, Wei-Seng Pang, Shek-Ling Abdullah, Julaihi Development and characterization of microsatellite markers in sawih tree (Duabanga moluccana Blume) using ISSR-suppression PCR techniques. |
| title | Development and characterization of microsatellite markers in sawih tree (Duabanga moluccana Blume) using ISSR-suppression PCR techniques. |
| title_full | Development and characterization of microsatellite markers in sawih tree (Duabanga moluccana Blume) using ISSR-suppression PCR techniques. |
| title_fullStr | Development and characterization of microsatellite markers in sawih tree (Duabanga moluccana Blume) using ISSR-suppression PCR techniques. |
| title_full_unstemmed | Development and characterization of microsatellite markers in sawih tree (Duabanga moluccana Blume) using ISSR-suppression PCR techniques. |
| title_short | Development and characterization of microsatellite markers in sawih tree (Duabanga moluccana Blume) using ISSR-suppression PCR techniques. |
| title_sort | development and characterization of microsatellite markers in sawih tree (duabanga moluccana blume) using issr-suppression pcr techniques. |
| topic | Q Science (General) QK Botany |
| url | http://ir.unimas.my/id/eprint/9756/ http://ir.unimas.my/id/eprint/9756/ http://ir.unimas.my/id/eprint/9756/ http://ir.unimas.my/id/eprint/9756/1/Kit%20Siong.pdf |