Lactobacillus infection related to midgut protein synthesis in the dengue vector Aedes albopictus: Platform of non-symbiont bacteria for the control of Aedes vectors
There have been a number of recent studies regarding the use of engineered insect symbiont bacteria for control of insect-borne diseases. However, searches for cultivable bacteria residing in the mosquito midgut have met with little success. The present study was conducted to evaluate the effects...
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| Format: | Article |
| Language: | English |
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JPVB
2010
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| Online Access: | http://ir.unimas.my/id/eprint/9599/ http://ir.unimas.my/id/eprint/9599/1/Lactobacillus%20infection%20related%20to%20midgut%20protein%20synthesis%20in%20the%20dengue%20vector%20Aedes%20albopictus%20%28abstract%29.pdf |
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| author | Hamady, Dieng Parimal, Talukder Tomomitsu, Satho Yukihiko, Nakashima Nobuhiro, Kashige Ikenna N., Nwachukwu Adzitey, Frederick Rahman G. M., Saifur Che Salmah, Md Rawi Abu Hassan, Ahmad Fumio, Miake |
| author_facet | Hamady, Dieng Parimal, Talukder Tomomitsu, Satho Yukihiko, Nakashima Nobuhiro, Kashige Ikenna N., Nwachukwu Adzitey, Frederick Rahman G. M., Saifur Che Salmah, Md Rawi Abu Hassan, Ahmad Fumio, Miake |
| author_sort | Hamady, Dieng |
| building | UNIMAS Institutional Repository |
| collection | Online Access |
| description | There have been a number of recent studies regarding the use of engineered insect symbiont bacteria
for control of insect-borne diseases. However, searches for cultivable bacteria residing in the mosquito
midgut have met with little success. The present study was conducted to evaluate the effects of the
human non-pathogenic lactobacilli on midgut protein synthesis in the dengue vector Aedes albopictus,
taking into account the ease of infection and its persistence. It was showed that antibiotic treatment of
mosquitoes did not prevent experimental infection, and readily reduced undesired infection, but did not
prevent re-infection by Lactobacillus spp. It suggests a high potential of colonization of a target vector
population under field conditions. Ingested lactobacilli remained in the female midgut for five days.
Lactobacillus reuteri (Lactobacillales: Lactobacillaceae) showed more specific proteins than
Lactobacillus brevis (Lactobacillales: Lactobacillaceae). Some proteins identified in L. brevis were
present at much higher levels in L. reuteri, while other proteins found in the latter were found at higher
levels in the former. Infection by L. brevis resulted in the absence of many proteins. In contrast, L.
reuteri infection resulted in increased levels of synthesis of a set of proteins present in the healthy
midguts. Both bacteria triggered changes in midgut protein synthesis, but activation was seen to a
greater extent with L. reuteri. These results are discussed in the context of paratransgenesis. |
| first_indexed | 2025-11-15T06:26:21Z |
| format | Article |
| id | unimas-9599 |
| institution | Universiti Malaysia Sarawak |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-15T06:26:21Z |
| publishDate | 2010 |
| publisher | JPVB |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | unimas-95992015-11-09T06:47:55Z http://ir.unimas.my/id/eprint/9599/ Lactobacillus infection related to midgut protein synthesis in the dengue vector Aedes albopictus: Platform of non-symbiont bacteria for the control of Aedes vectors Hamady, Dieng Parimal, Talukder Tomomitsu, Satho Yukihiko, Nakashima Nobuhiro, Kashige Ikenna N., Nwachukwu Adzitey, Frederick Rahman G. M., Saifur Che Salmah, Md Rawi Abu Hassan, Ahmad Fumio, Miake QR Microbiology There have been a number of recent studies regarding the use of engineered insect symbiont bacteria for control of insect-borne diseases. However, searches for cultivable bacteria residing in the mosquito midgut have met with little success. The present study was conducted to evaluate the effects of the human non-pathogenic lactobacilli on midgut protein synthesis in the dengue vector Aedes albopictus, taking into account the ease of infection and its persistence. It was showed that antibiotic treatment of mosquitoes did not prevent experimental infection, and readily reduced undesired infection, but did not prevent re-infection by Lactobacillus spp. It suggests a high potential of colonization of a target vector population under field conditions. Ingested lactobacilli remained in the female midgut for five days. Lactobacillus reuteri (Lactobacillales: Lactobacillaceae) showed more specific proteins than Lactobacillus brevis (Lactobacillales: Lactobacillaceae). Some proteins identified in L. brevis were present at much higher levels in L. reuteri, while other proteins found in the latter were found at higher levels in the former. Infection by L. brevis resulted in the absence of many proteins. In contrast, L. reuteri infection resulted in increased levels of synthesis of a set of proteins present in the healthy midguts. Both bacteria triggered changes in midgut protein synthesis, but activation was seen to a greater extent with L. reuteri. These results are discussed in the context of paratransgenesis. JPVB 2010 Article NonPeerReviewed text en http://ir.unimas.my/id/eprint/9599/1/Lactobacillus%20infection%20related%20to%20midgut%20protein%20synthesis%20in%20the%20dengue%20vector%20Aedes%20albopictus%20%28abstract%29.pdf Hamady, Dieng and Parimal, Talukder and Tomomitsu, Satho and Yukihiko, Nakashima and Nobuhiro, Kashige and Ikenna N., Nwachukwu and Adzitey, Frederick and Rahman G. M., Saifur and Che Salmah, Md Rawi and Abu Hassan, Ahmad and Fumio, Miake (2010) Lactobacillus infection related to midgut protein synthesis in the dengue vector Aedes albopictus: Platform of non-symbiont bacteria for the control of Aedes vectors. Journal of Parasitology and Vector Biology, 2 (2). 014-021. ISSN 2141-2510 http://www.academicjournals.org/JPVB |
| spellingShingle | QR Microbiology Hamady, Dieng Parimal, Talukder Tomomitsu, Satho Yukihiko, Nakashima Nobuhiro, Kashige Ikenna N., Nwachukwu Adzitey, Frederick Rahman G. M., Saifur Che Salmah, Md Rawi Abu Hassan, Ahmad Fumio, Miake Lactobacillus infection related to midgut protein synthesis in the dengue vector Aedes albopictus: Platform of non-symbiont bacteria for the control of Aedes vectors |
| title | Lactobacillus infection related to midgut protein synthesis in the dengue vector Aedes albopictus: Platform of non-symbiont bacteria for the control of Aedes vectors |
| title_full | Lactobacillus infection related to midgut protein synthesis in the dengue vector Aedes albopictus: Platform of non-symbiont bacteria for the control of Aedes vectors |
| title_fullStr | Lactobacillus infection related to midgut protein synthesis in the dengue vector Aedes albopictus: Platform of non-symbiont bacteria for the control of Aedes vectors |
| title_full_unstemmed | Lactobacillus infection related to midgut protein synthesis in the dengue vector Aedes albopictus: Platform of non-symbiont bacteria for the control of Aedes vectors |
| title_short | Lactobacillus infection related to midgut protein synthesis in the dengue vector Aedes albopictus: Platform of non-symbiont bacteria for the control of Aedes vectors |
| title_sort | lactobacillus infection related to midgut protein synthesis in the dengue vector aedes albopictus: platform of non-symbiont bacteria for the control of aedes vectors |
| topic | QR Microbiology |
| url | http://ir.unimas.my/id/eprint/9599/ http://ir.unimas.my/id/eprint/9599/ http://ir.unimas.my/id/eprint/9599/1/Lactobacillus%20infection%20related%20to%20midgut%20protein%20synthesis%20in%20the%20dengue%20vector%20Aedes%20albopictus%20%28abstract%29.pdf |