A generic assay for whole-genome amplification and deep sequencingof enterovirus A71
Enterovirus A71 (EV-A71) has emerged as the most important cause of large outbreaks of severe and sometimes fatal hand, foot and mouth disease (HFMD) across the Asia-Pacific region. EV-A71 outbreaks have been associated with (sub)genogroup switches, sometimes accompanied by recombination events. Und...
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| Format: | Article |
| Language: | English |
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Elsevier B.V
2015
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| Online Access: | http://ir.unimas.my/id/eprint/9313/ http://ir.unimas.my/id/eprint/9313/1/A%20generic.pdf |
| _version_ | 1848836537511313408 |
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| author | Le, Van Tan Nguyen, Thi Kim Tuyen Tran, Tan Thanh Tran, Thuy Ngan Hoang, Minh Tu Van |
| author_facet | Le, Van Tan Nguyen, Thi Kim Tuyen Tran, Tan Thanh Tran, Thuy Ngan Hoang, Minh Tu Van |
| author_sort | Le, Van Tan |
| building | UNIMAS Institutional Repository |
| collection | Online Access |
| description | Enterovirus A71 (EV-A71) has emerged as the most important cause of large outbreaks of severe and sometimes fatal hand, foot and mouth disease (HFMD) across the Asia-Pacific region. EV-A71 outbreaks have been associated with (sub)genogroup switches, sometimes accompanied by recombination events. Understanding EV-A71 population dynamics is therefore essential for understanding this emerging infection, and may provide pivotal information for vaccine development. Despite the public health burden of EV-A71, relatively few EV-A71 complete-genome sequences are available for analysis and from limited geographical localities. The availability of an efficient procedure for whole-genome sequencing would stimulate effort to generate more viral sequence data. Herein, we report for the first time the development of a next-generation sequencing based protocol for whole-genome sequencing of EV-A71 directly from clinical specimens. We were able to sequence viruses of subgenogroup C4 and B5, while RNA from culture materials of diverse EV-A71 subgenogroups belonging to both genogroup B and C was successfully amplified. The nature of intra-host genetic diversity was explored in 22 clinical samples, revealing 107 positions carrying minor variants (ranging from 0 to 15 variants per sample). Our analysis of EV-A71 strains sampled in 2013 showed that they all belonged to subgenogroup B5, representing the first report of this subgenogroup in Vietnam. In conclusion, we have successfully developed a high-throughput next-generation sequencing-based assay for whole-genome sequencing of EV-A71 from clinical samples. |
| first_indexed | 2025-11-15T06:25:21Z |
| format | Article |
| id | unimas-9313 |
| institution | Universiti Malaysia Sarawak |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-15T06:25:21Z |
| publishDate | 2015 |
| publisher | Elsevier B.V |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | unimas-93132022-01-26T08:46:51Z http://ir.unimas.my/id/eprint/9313/ A generic assay for whole-genome amplification and deep sequencingof enterovirus A71 Le, Van Tan Nguyen, Thi Kim Tuyen Tran, Tan Thanh Tran, Thuy Ngan Hoang, Minh Tu Van RA Public aspects of medicine RA0421 Public health. Hygiene. Preventive Medicine RB Pathology Enterovirus A71 (EV-A71) has emerged as the most important cause of large outbreaks of severe and sometimes fatal hand, foot and mouth disease (HFMD) across the Asia-Pacific region. EV-A71 outbreaks have been associated with (sub)genogroup switches, sometimes accompanied by recombination events. Understanding EV-A71 population dynamics is therefore essential for understanding this emerging infection, and may provide pivotal information for vaccine development. Despite the public health burden of EV-A71, relatively few EV-A71 complete-genome sequences are available for analysis and from limited geographical localities. The availability of an efficient procedure for whole-genome sequencing would stimulate effort to generate more viral sequence data. Herein, we report for the first time the development of a next-generation sequencing based protocol for whole-genome sequencing of EV-A71 directly from clinical specimens. We were able to sequence viruses of subgenogroup C4 and B5, while RNA from culture materials of diverse EV-A71 subgenogroups belonging to both genogroup B and C was successfully amplified. The nature of intra-host genetic diversity was explored in 22 clinical samples, revealing 107 positions carrying minor variants (ranging from 0 to 15 variants per sample). Our analysis of EV-A71 strains sampled in 2013 showed that they all belonged to subgenogroup B5, representing the first report of this subgenogroup in Vietnam. In conclusion, we have successfully developed a high-throughput next-generation sequencing-based assay for whole-genome sequencing of EV-A71 from clinical samples. Elsevier B.V 2015 Article NonPeerReviewed text en http://ir.unimas.my/id/eprint/9313/1/A%20generic.pdf Le, Van Tan and Nguyen, Thi Kim Tuyen and Tran, Tan Thanh and Tran, Thuy Ngan and Hoang, Minh Tu Van (2015) A generic assay for whole-genome amplification and deep sequencingof enterovirus A71. Journal of Virological Methods, 215-21. pp. 30-36. ISSN 0166-0934 http://www.scopus.com/inward/record.url?eid=2-s2.0-84925010124&partnerID=40&md5=ab62ea82d5458a4227be1f0982cef092 http://10.1016/j.jviromet.2015.02.011 |
| spellingShingle | RA Public aspects of medicine RA0421 Public health. Hygiene. Preventive Medicine RB Pathology Le, Van Tan Nguyen, Thi Kim Tuyen Tran, Tan Thanh Tran, Thuy Ngan Hoang, Minh Tu Van A generic assay for whole-genome amplification and deep sequencingof enterovirus A71 |
| title | A generic assay for whole-genome amplification and deep sequencingof enterovirus A71 |
| title_full | A generic assay for whole-genome amplification and deep sequencingof enterovirus A71 |
| title_fullStr | A generic assay for whole-genome amplification and deep sequencingof enterovirus A71 |
| title_full_unstemmed | A generic assay for whole-genome amplification and deep sequencingof enterovirus A71 |
| title_short | A generic assay for whole-genome amplification and deep sequencingof enterovirus A71 |
| title_sort | generic assay for whole-genome amplification and deep sequencingof enterovirus a71 |
| topic | RA Public aspects of medicine RA0421 Public health. Hygiene. Preventive Medicine RB Pathology |
| url | http://ir.unimas.my/id/eprint/9313/ http://ir.unimas.my/id/eprint/9313/ http://ir.unimas.my/id/eprint/9313/ http://ir.unimas.my/id/eprint/9313/1/A%20generic.pdf |