Expression of Recombinant E1 Glycoprotein of Chikungunya Virus in Baculovirus Expression System
Chikungunya is an acute febrile illness caused by chikungunya virus (CHIKV). In this study, the envelope E1 gene of CHIKV was cloned and expressed in a baculovirus system. The recombinant E1 protein with N-term 6-His residues protein was successfully expressed and purified as confirmed by SDS-PAG...
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| Format: | Article |
| Language: | English |
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Hibiscus Publisher
2015
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| Online Access: | http://ir.unimas.my/id/eprint/9288/ http://ir.unimas.my/id/eprint/9288/1/Expression%20of%20Recombinant%20E1%20Glycoprotein%20of%20Chikungunya%20Virus%20in%20Baculovirus%20Expression%20System%20%28abstract0.pdf |
| _version_ | 1848836531090882560 |
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| author | Henry Sum, Magdline Sia Andrew, Anna Aren Maling, Milda |
| author_facet | Henry Sum, Magdline Sia Andrew, Anna Aren Maling, Milda |
| author_sort | Henry Sum, Magdline Sia |
| building | UNIMAS Institutional Repository |
| collection | Online Access |
| description | Chikungunya is an acute febrile illness caused by chikungunya virus (CHIKV). In this study, the
envelope E1 gene of CHIKV was cloned and expressed in a baculovirus system. The
recombinant E1 protein with N-term 6-His residues protein was successfully expressed and
purified as confirmed by SDS-PAGE and western blot analysis. The seroreactivity of the
recombinant protein was evaluated in immunoassay for anti-CHIKV IgM and IgG antibodies.
The recombinant antigen showed 69% sensitivity and 100% specificity for anti-CHIKV IgG by
dot blot assay. Detection of anti-CHIKV IgM by dot assay showed 79% sensitivity and 100%
specificity. No cross reactivity of the antigen was observed with anti-dengue virus serum
samples. The results strongly support that the recombinant E1 protein has potential to be used as
diagnostic antigen. The used of the antigen in a dot blot assay gives an advantage for laboratory
detection without the need of any specialised equipment. |
| first_indexed | 2025-11-15T06:25:14Z |
| format | Article |
| id | unimas-9288 |
| institution | Universiti Malaysia Sarawak |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-15T06:25:14Z |
| publishDate | 2015 |
| publisher | Hibiscus Publisher |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | unimas-92882015-11-03T07:23:22Z http://ir.unimas.my/id/eprint/9288/ Expression of Recombinant E1 Glycoprotein of Chikungunya Virus in Baculovirus Expression System Henry Sum, Magdline Sia Andrew, Anna Aren Maling, Milda Q Science (General) QR Microbiology QR180 Immunology QR355 Virology R Medicine (General) Chikungunya is an acute febrile illness caused by chikungunya virus (CHIKV). In this study, the envelope E1 gene of CHIKV was cloned and expressed in a baculovirus system. The recombinant E1 protein with N-term 6-His residues protein was successfully expressed and purified as confirmed by SDS-PAGE and western blot analysis. The seroreactivity of the recombinant protein was evaluated in immunoassay for anti-CHIKV IgM and IgG antibodies. The recombinant antigen showed 69% sensitivity and 100% specificity for anti-CHIKV IgG by dot blot assay. Detection of anti-CHIKV IgM by dot assay showed 79% sensitivity and 100% specificity. No cross reactivity of the antigen was observed with anti-dengue virus serum samples. The results strongly support that the recombinant E1 protein has potential to be used as diagnostic antigen. The used of the antigen in a dot blot assay gives an advantage for laboratory detection without the need of any specialised equipment. Hibiscus Publisher 2015-09-15 Article PeerReviewed text en http://ir.unimas.my/id/eprint/9288/1/Expression%20of%20Recombinant%20E1%20Glycoprotein%20of%20Chikungunya%20Virus%20in%20Baculovirus%20Expression%20System%20%28abstract0.pdf Henry Sum, Magdline Sia and Andrew, Anna and Aren Maling, Milda (2015) Expression of Recombinant E1 Glycoprotein of Chikungunya Virus in Baculovirus Expression System. Journal of biochemistry, microbiology and biotechnology, 3 (1). pp. 26-29. ISSN 2289-5779 http://www.researchgate.net/publication/283352293_Expression_of_Recombinant_E1_Glycoprotein_of_Chikungunya_Virus_in_Baculovirus_Expression_System |
| spellingShingle | Q Science (General) QR Microbiology QR180 Immunology QR355 Virology R Medicine (General) Henry Sum, Magdline Sia Andrew, Anna Aren Maling, Milda Expression of Recombinant E1 Glycoprotein of Chikungunya Virus in Baculovirus Expression System |
| title | Expression of Recombinant E1 Glycoprotein of Chikungunya Virus in Baculovirus Expression System |
| title_full | Expression of Recombinant E1 Glycoprotein of Chikungunya Virus in Baculovirus Expression System |
| title_fullStr | Expression of Recombinant E1 Glycoprotein of Chikungunya Virus in Baculovirus Expression System |
| title_full_unstemmed | Expression of Recombinant E1 Glycoprotein of Chikungunya Virus in Baculovirus Expression System |
| title_short | Expression of Recombinant E1 Glycoprotein of Chikungunya Virus in Baculovirus Expression System |
| title_sort | expression of recombinant e1 glycoprotein of chikungunya virus in baculovirus expression system |
| topic | Q Science (General) QR Microbiology QR180 Immunology QR355 Virology R Medicine (General) |
| url | http://ir.unimas.my/id/eprint/9288/ http://ir.unimas.my/id/eprint/9288/ http://ir.unimas.my/id/eprint/9288/1/Expression%20of%20Recombinant%20E1%20Glycoprotein%20of%20Chikungunya%20Virus%20in%20Baculovirus%20Expression%20System%20%28abstract0.pdf |